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EMILIN2 (Elastin microfibril interface located protein), potential modifier of thrombosis.

Sa Q, Hoover-Plow JL - Thromb J (2011)

Bottom Line: Elastin microfibril interface located protein 2 (EMILIN2) is an extracellular glycoprotein associated with cardiovascular development.EMILIN2 was identified with cells and extracellular matrix by immunohistochemistry in the carotid and aorta.These results suggest EMILIN2 could play a role in thrombosis as a constituent of the vessel wall and/or a component of the thrombus.

View Article: PubMed Central - HTML - PubMed

Affiliation: Joseph J, Jacobs Center For Thrombosis and Vascular Biology, Department of Cardiovascular Medicine and Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA. hooverj@ccf.org.

ABSTRACT

Background: Elastin microfibril interface located protein 2 (EMILIN2) is an extracellular glycoprotein associated with cardiovascular development. While other EMILIN proteins are reported to play a role in elastogenesis and coagulation, little is known about EMILIN2 function in the cardiovascular system. The objective of this study was to determine whether EMILIN2 could play a role in thrombosis.

Results: EMILIN2 mRNA was expressed in 8 wk old C57BL/6J mice in lung, heart, aorta and bone marrow, with the highest expression in bone marrow. In mouse cells, EMILIN2 mRNA expression in macrophages was higher than expression in endothelial cells and fibroblasts. EMILIN2 was identified with cells and extracellular matrix by immunohistochemistry in the carotid and aorta. After carotid ferric chloride injury, EMILIN2 was abundantly expressed in the thrombus and inhibition of EMILIN2 increased platelet de-aggregation after ADP-stimulated platelet aggregation.

Conclusions: These results suggest EMILIN2 could play a role in thrombosis as a constituent of the vessel wall and/or a component of the thrombus.

No MeSH data available.


Related in: MedlinePlus

Emilin2 mRNA Expression in Tissues and Mouse Cells. GAPDH from the same cDNA samples used as endogenous control. A. Expression of Emilin2 in lung, heart, aorta and bone marrow cells was determined by real-time PCR. Aorta, n = 3; others, n = 5. The mRNA levels are presented as fold change relative to lung. The values are mean ± SEM n = 3-5. B. Expression of Emilin2 in mouse cell lines. The mRNA levels are presented as fold change relative to EOMA. EOMA, mouse endothelial cell line; NIH3T3, mouse fibroblast cell line; Raw264.7, mouse macrophage-like cell line. The values are mean ± SEM of 3 independent experiments. Statistical differences compared to EOMA were determined by ANOVA and Newman-Kuels post-test. **P < 0.01, ***P < 0.001.
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Figure 2: Emilin2 mRNA Expression in Tissues and Mouse Cells. GAPDH from the same cDNA samples used as endogenous control. A. Expression of Emilin2 in lung, heart, aorta and bone marrow cells was determined by real-time PCR. Aorta, n = 3; others, n = 5. The mRNA levels are presented as fold change relative to lung. The values are mean ± SEM n = 3-5. B. Expression of Emilin2 in mouse cell lines. The mRNA levels are presented as fold change relative to EOMA. EOMA, mouse endothelial cell line; NIH3T3, mouse fibroblast cell line; Raw264.7, mouse macrophage-like cell line. The values are mean ± SEM of 3 independent experiments. Statistical differences compared to EOMA were determined by ANOVA and Newman-Kuels post-test. **P < 0.01, ***P < 0.001.

Mentions: The mRNA expression of Emilin2 was determined by quantitative real-time PCR in lung, heart, aorta and bone marrow cells (Figure 2A). The expression in the bone marrow was 20-fold higher than in the lung. This suggests that bone marrow is a major source of Emilin2 and is unlike Emilin1 expression [21] where the major source is the vessel wall.


EMILIN2 (Elastin microfibril interface located protein), potential modifier of thrombosis.

Sa Q, Hoover-Plow JL - Thromb J (2011)

Emilin2 mRNA Expression in Tissues and Mouse Cells. GAPDH from the same cDNA samples used as endogenous control. A. Expression of Emilin2 in lung, heart, aorta and bone marrow cells was determined by real-time PCR. Aorta, n = 3; others, n = 5. The mRNA levels are presented as fold change relative to lung. The values are mean ± SEM n = 3-5. B. Expression of Emilin2 in mouse cell lines. The mRNA levels are presented as fold change relative to EOMA. EOMA, mouse endothelial cell line; NIH3T3, mouse fibroblast cell line; Raw264.7, mouse macrophage-like cell line. The values are mean ± SEM of 3 independent experiments. Statistical differences compared to EOMA were determined by ANOVA and Newman-Kuels post-test. **P < 0.01, ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113922&req=5

Figure 2: Emilin2 mRNA Expression in Tissues and Mouse Cells. GAPDH from the same cDNA samples used as endogenous control. A. Expression of Emilin2 in lung, heart, aorta and bone marrow cells was determined by real-time PCR. Aorta, n = 3; others, n = 5. The mRNA levels are presented as fold change relative to lung. The values are mean ± SEM n = 3-5. B. Expression of Emilin2 in mouse cell lines. The mRNA levels are presented as fold change relative to EOMA. EOMA, mouse endothelial cell line; NIH3T3, mouse fibroblast cell line; Raw264.7, mouse macrophage-like cell line. The values are mean ± SEM of 3 independent experiments. Statistical differences compared to EOMA were determined by ANOVA and Newman-Kuels post-test. **P < 0.01, ***P < 0.001.
Mentions: The mRNA expression of Emilin2 was determined by quantitative real-time PCR in lung, heart, aorta and bone marrow cells (Figure 2A). The expression in the bone marrow was 20-fold higher than in the lung. This suggests that bone marrow is a major source of Emilin2 and is unlike Emilin1 expression [21] where the major source is the vessel wall.

Bottom Line: Elastin microfibril interface located protein 2 (EMILIN2) is an extracellular glycoprotein associated with cardiovascular development.EMILIN2 was identified with cells and extracellular matrix by immunohistochemistry in the carotid and aorta.These results suggest EMILIN2 could play a role in thrombosis as a constituent of the vessel wall and/or a component of the thrombus.

View Article: PubMed Central - HTML - PubMed

Affiliation: Joseph J, Jacobs Center For Thrombosis and Vascular Biology, Department of Cardiovascular Medicine and Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA. hooverj@ccf.org.

ABSTRACT

Background: Elastin microfibril interface located protein 2 (EMILIN2) is an extracellular glycoprotein associated with cardiovascular development. While other EMILIN proteins are reported to play a role in elastogenesis and coagulation, little is known about EMILIN2 function in the cardiovascular system. The objective of this study was to determine whether EMILIN2 could play a role in thrombosis.

Results: EMILIN2 mRNA was expressed in 8 wk old C57BL/6J mice in lung, heart, aorta and bone marrow, with the highest expression in bone marrow. In mouse cells, EMILIN2 mRNA expression in macrophages was higher than expression in endothelial cells and fibroblasts. EMILIN2 was identified with cells and extracellular matrix by immunohistochemistry in the carotid and aorta. After carotid ferric chloride injury, EMILIN2 was abundantly expressed in the thrombus and inhibition of EMILIN2 increased platelet de-aggregation after ADP-stimulated platelet aggregation.

Conclusions: These results suggest EMILIN2 could play a role in thrombosis as a constituent of the vessel wall and/or a component of the thrombus.

No MeSH data available.


Related in: MedlinePlus