Limits...
Lingual haemangiosarcoma in a crossbred dog.

Owen LJ, Grierson JM, Patterson-Kane JC, Baines SJ - Ir Vet J (2006)

Bottom Line: An eight-year-old, male neutered, crossbred dog was presented for investigation of a lingual mass of four months duration.Oral examination revealed a 7 cm × 5 cm soft, fluctuant mass at the caudal aspect of the tongue.Lingual haemangiosarcoma was diagnosed on histopathological examination of multiple biopsy samples, with confirmation of the vascular endothelial origin of tumour cells by positive immunolabelling for factor VIII-related antigen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Small Animal Hospital, Department of Clinical Veterinary Science, Langford House, Bristol, UK. laura.owen@bristol.ac.uk.

ABSTRACT
An eight-year-old, male neutered, crossbred dog was presented for investigation of a lingual mass of four months duration. Oral examination revealed a 7 cm × 5 cm soft, fluctuant mass at the caudal aspect of the tongue. Ultrasound examination of the mass demonstrated mixed echogenicity, with cavitations containing hypoechoic and anechoic regions. Lingual haemangiosarcoma was diagnosed on histopathological examination of multiple biopsy samples, with confirmation of the vascular endothelial origin of tumour cells by positive immunolabelling for factor VIII-related antigen.

No MeSH data available.


Related in: MedlinePlus

Tumour cells including those lining vascular spaces (asterisks) and those containing mitotic figures (arrows) show positive granular cytoplasmic staining for factor VIII-related antigen. (Two-layer indirect immunoperoxidase staining. Bar = 250 microns.).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3113891&req=5

Figure 5: Tumour cells including those lining vascular spaces (asterisks) and those containing mitotic figures (arrows) show positive granular cytoplasmic staining for factor VIII-related antigen. (Two-layer indirect immunoperoxidase staining. Bar = 250 microns.).

Mentions: Biopsy specimens were fixed in 10% neutral buffered formalin and were processed for histological examination by routine methods and were embedded in paraffin wax. Sections (5 μm) were cut and mounted on glass slides and stained with haematoxylin and eosin. Further sections were dewaxed and rehydrated by routine methods for immunohistochemical staining. Antigen retrieval was accomplished by protease digestion at 37°C, and the sections were labelled with rabbit polyclonal antibody to factor VIII-related antigen (von Willebrand factor; Dako A/S, Denmark) at a 1 in 4000 dilution. Histologically, tumour cells comprising these sections were arranged in sheets and lined numerous irregularly shaped blood-filled spaces. The tumour cells were round or polygonal, with central, bulging, round nuclei. There was moderate variation in nuclear size with up to six mitotic figures per high power (X400) field (Figure 4). Large areas of tumour cell necrosis with haemorrhage were noted. The tumour cells showed diffuse, strong positive cytoplasmic labelling for factor VIII-related antigen (Figure 5). The histological and immunohistochemical findings were consistent with the diagnosis of haemangiosarcoma.


Lingual haemangiosarcoma in a crossbred dog.

Owen LJ, Grierson JM, Patterson-Kane JC, Baines SJ - Ir Vet J (2006)

Tumour cells including those lining vascular spaces (asterisks) and those containing mitotic figures (arrows) show positive granular cytoplasmic staining for factor VIII-related antigen. (Two-layer indirect immunoperoxidase staining. Bar = 250 microns.).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3113891&req=5

Figure 5: Tumour cells including those lining vascular spaces (asterisks) and those containing mitotic figures (arrows) show positive granular cytoplasmic staining for factor VIII-related antigen. (Two-layer indirect immunoperoxidase staining. Bar = 250 microns.).
Mentions: Biopsy specimens were fixed in 10% neutral buffered formalin and were processed for histological examination by routine methods and were embedded in paraffin wax. Sections (5 μm) were cut and mounted on glass slides and stained with haematoxylin and eosin. Further sections were dewaxed and rehydrated by routine methods for immunohistochemical staining. Antigen retrieval was accomplished by protease digestion at 37°C, and the sections were labelled with rabbit polyclonal antibody to factor VIII-related antigen (von Willebrand factor; Dako A/S, Denmark) at a 1 in 4000 dilution. Histologically, tumour cells comprising these sections were arranged in sheets and lined numerous irregularly shaped blood-filled spaces. The tumour cells were round or polygonal, with central, bulging, round nuclei. There was moderate variation in nuclear size with up to six mitotic figures per high power (X400) field (Figure 4). Large areas of tumour cell necrosis with haemorrhage were noted. The tumour cells showed diffuse, strong positive cytoplasmic labelling for factor VIII-related antigen (Figure 5). The histological and immunohistochemical findings were consistent with the diagnosis of haemangiosarcoma.

Bottom Line: An eight-year-old, male neutered, crossbred dog was presented for investigation of a lingual mass of four months duration.Oral examination revealed a 7 cm × 5 cm soft, fluctuant mass at the caudal aspect of the tongue.Lingual haemangiosarcoma was diagnosed on histopathological examination of multiple biopsy samples, with confirmation of the vascular endothelial origin of tumour cells by positive immunolabelling for factor VIII-related antigen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Small Animal Hospital, Department of Clinical Veterinary Science, Langford House, Bristol, UK. laura.owen@bristol.ac.uk.

ABSTRACT
An eight-year-old, male neutered, crossbred dog was presented for investigation of a lingual mass of four months duration. Oral examination revealed a 7 cm × 5 cm soft, fluctuant mass at the caudal aspect of the tongue. Ultrasound examination of the mass demonstrated mixed echogenicity, with cavitations containing hypoechoic and anechoic regions. Lingual haemangiosarcoma was diagnosed on histopathological examination of multiple biopsy samples, with confirmation of the vascular endothelial origin of tumour cells by positive immunolabelling for factor VIII-related antigen.

No MeSH data available.


Related in: MedlinePlus