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Understanding Streptococcus suis serotype 2 infection in pigs through a transcriptional approach.

Liu M, Fang L, Tan C, Long T, Chen H, Xiao S - BMC Genomics (2011)

Bottom Line: The DE genes involved in the immune response included genes related to the inflammatory response (CD163), the innate immune response (TLR2, TLR4, MYD88, TIRAP), cell adhesion (CD34, SELE, SELL, SELP, ICAM-1, ICAM-2, VCAM-1), antigen processing and presentation (MHC protein complex) and angiogenesis (VEGF), together with genes encoding cytokines (interleukins).Five selected genes were validated by qRT-PCR analysis.This new information will form the foundation of future investigations into the pathogenesis of S. suis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Animal Pathogens, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT

Background: Streptococcus suis serotype 2 (S. suis 2) is an important pathogen of pigs. S suis 2 infections have high mortality rates and are characterized by meningitis, septicemia and pneumonia. S. suis 2 is also an emerging zoonotic agent and can infect humans that are exposed to pigs or their by-products. To increase our knowledge of the pathogenesis of meningitis, septicemia and pneumonia in pigs caused by S. suis 2, we profiled the response of peripheral blood mononuclear cells (PBMC), brain and lung tissues to infection with S. suis 2 strain SC19 using the Affymetrix Porcine Genome Array.

Results: A total of 3,002 differentially expressed transcripts were identified in the three tissues, including 417 unique genes in brain, 210 in lung and 213 in PBMC. These genes showed differential expression (DE) patterns on analysis by visualization and integrated discovery (DAVID). The DE genes involved in the immune response included genes related to the inflammatory response (CD163), the innate immune response (TLR2, TLR4, MYD88, TIRAP), cell adhesion (CD34, SELE, SELL, SELP, ICAM-1, ICAM-2, VCAM-1), antigen processing and presentation (MHC protein complex) and angiogenesis (VEGF), together with genes encoding cytokines (interleukins). Five selected genes were validated by qRT-PCR analysis.

Conclusions: We studied the response to infection with S. suis 2 strain SC19 by microarray analysis. Our findings confirmed some genes identified in previous studies and discovered numerous additional genes that potentially function in S. suis 2 infections in vivo. This new information will form the foundation of future investigations into the pathogenesis of S. suis.

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Related in: MedlinePlus

Number of genes expressed differentially in brain, lung and PBMC. The number of genes found to be differentially expressed in each of the tissues is shown in the circles. The grey circle represents the number of genes in the brain, tinted grey represents the PBMC and deep grey circle represents the lung.A. Number of DE genes including up-regulated and down-regulated.B. Number of up-regulated genes.C. Number of down-regulated genes.
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Figure 1: Number of genes expressed differentially in brain, lung and PBMC. The number of genes found to be differentially expressed in each of the tissues is shown in the circles. The grey circle represents the number of genes in the brain, tinted grey represents the PBMC and deep grey circle represents the lung.A. Number of DE genes including up-regulated and down-regulated.B. Number of up-regulated genes.C. Number of down-regulated genes.

Mentions: In this study, differential gene expression was investigated among the three tissues studied (PBMC, brain and lung). Transcript analysis indicated that at least 48.19% and up to 67.89% of the probe sets were well detected in all 18 samples. The criteria of fold change (FC) ≥ 2 and p value ≤0.05 were considered to indicate up-regulation; FC ≤ 0.5 and p ≤ 0.05 were considered to represent down-regulation. Genes whose relative transcription levels were between 0.5 and 2 were considered to show no significant change. As shown in Figure 1, 1608 genes in the brain showed changes, including 344 genes up-regulated and 1264 genes down-regulated. There were 617 genes were changed in the lung, 293 up-regulated and 324 down-regulated and 685 genes were changed in PBMC, 403 up-regulated and 282 down-regulated. The expression of 31 genes was found to change in response to infection in all three tissues, comprising 26 genes up-regulated and 5 genes down-regulated.


Understanding Streptococcus suis serotype 2 infection in pigs through a transcriptional approach.

Liu M, Fang L, Tan C, Long T, Chen H, Xiao S - BMC Genomics (2011)

Number of genes expressed differentially in brain, lung and PBMC. The number of genes found to be differentially expressed in each of the tissues is shown in the circles. The grey circle represents the number of genes in the brain, tinted grey represents the PBMC and deep grey circle represents the lung.A. Number of DE genes including up-regulated and down-regulated.B. Number of up-regulated genes.C. Number of down-regulated genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113788&req=5

Figure 1: Number of genes expressed differentially in brain, lung and PBMC. The number of genes found to be differentially expressed in each of the tissues is shown in the circles. The grey circle represents the number of genes in the brain, tinted grey represents the PBMC and deep grey circle represents the lung.A. Number of DE genes including up-regulated and down-regulated.B. Number of up-regulated genes.C. Number of down-regulated genes.
Mentions: In this study, differential gene expression was investigated among the three tissues studied (PBMC, brain and lung). Transcript analysis indicated that at least 48.19% and up to 67.89% of the probe sets were well detected in all 18 samples. The criteria of fold change (FC) ≥ 2 and p value ≤0.05 were considered to indicate up-regulation; FC ≤ 0.5 and p ≤ 0.05 were considered to represent down-regulation. Genes whose relative transcription levels were between 0.5 and 2 were considered to show no significant change. As shown in Figure 1, 1608 genes in the brain showed changes, including 344 genes up-regulated and 1264 genes down-regulated. There were 617 genes were changed in the lung, 293 up-regulated and 324 down-regulated and 685 genes were changed in PBMC, 403 up-regulated and 282 down-regulated. The expression of 31 genes was found to change in response to infection in all three tissues, comprising 26 genes up-regulated and 5 genes down-regulated.

Bottom Line: The DE genes involved in the immune response included genes related to the inflammatory response (CD163), the innate immune response (TLR2, TLR4, MYD88, TIRAP), cell adhesion (CD34, SELE, SELL, SELP, ICAM-1, ICAM-2, VCAM-1), antigen processing and presentation (MHC protein complex) and angiogenesis (VEGF), together with genes encoding cytokines (interleukins).Five selected genes were validated by qRT-PCR analysis.This new information will form the foundation of future investigations into the pathogenesis of S. suis.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Animal Pathogens, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT

Background: Streptococcus suis serotype 2 (S. suis 2) is an important pathogen of pigs. S suis 2 infections have high mortality rates and are characterized by meningitis, septicemia and pneumonia. S. suis 2 is also an emerging zoonotic agent and can infect humans that are exposed to pigs or their by-products. To increase our knowledge of the pathogenesis of meningitis, septicemia and pneumonia in pigs caused by S. suis 2, we profiled the response of peripheral blood mononuclear cells (PBMC), brain and lung tissues to infection with S. suis 2 strain SC19 using the Affymetrix Porcine Genome Array.

Results: A total of 3,002 differentially expressed transcripts were identified in the three tissues, including 417 unique genes in brain, 210 in lung and 213 in PBMC. These genes showed differential expression (DE) patterns on analysis by visualization and integrated discovery (DAVID). The DE genes involved in the immune response included genes related to the inflammatory response (CD163), the innate immune response (TLR2, TLR4, MYD88, TIRAP), cell adhesion (CD34, SELE, SELL, SELP, ICAM-1, ICAM-2, VCAM-1), antigen processing and presentation (MHC protein complex) and angiogenesis (VEGF), together with genes encoding cytokines (interleukins). Five selected genes were validated by qRT-PCR analysis.

Conclusions: We studied the response to infection with S. suis 2 strain SC19 by microarray analysis. Our findings confirmed some genes identified in previous studies and discovered numerous additional genes that potentially function in S. suis 2 infections in vivo. This new information will form the foundation of future investigations into the pathogenesis of S. suis.

Show MeSH
Related in: MedlinePlus