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Characterization of age-related gene expression profiling in bone marrow and epididymal adipocytes.

Liu LF, Shen WJ, Ueno M, Patel S, Kraemer FB - BMC Genomics (2011)

Bottom Line: Age had a substantial effect on genes associated with mitochondria function and inflammation in bone marrow adipocytes.Twenty seven genes were significantly changed with age in both adipocyte depots.Among these genes, IL6 and GPR109A were significantly reduced with age in both adipocyte depots.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Endocrinology, Stanford University, CA 94305-5103, USA.

ABSTRACT

Background: While an increase in bone marrow adiposity is associated with age-related bone disease, the function of bone marrow adipocytes has not been studied. The aim of this study was to characterize and compare the age-related gene expression profiles in bone marrow adipocytes and epididymal adipocytes.

Results: A total of 3918 (13.7%) genes were differentially expressed in bone marrow adipocytes compared to epididymal adipocytes. Bone marrow adipocytes revealed a distinct gene profile with low expression of adipocyte-specific genes peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid binding protein 4 (FABP4), perilipin (Plin1), adipsin (CFD) and high expression of genes associated with early adipocyte differentiation (CCAAT/enhancer binding protein beta (C/EBPβ), regulator of G-protein signaling 2 (RGS2). In addition, a number of genes including secreted frizzled related protein 4 (SFRP4), tumor necrosis factor α (TNFα), transforming growth factor beta 1(TGFβ1), G-protein coupled receptor 109A (GPR109A) and interleukin 6 (IL-6), that could affect adipose-derived signaling to bone are markedly increased in bone marrow adipocytes. Age had a substantial effect on genes associated with mitochondria function and inflammation in bone marrow adipocytes. Twenty seven genes were significantly changed with age in both adipocyte depots. Among these genes, IL6 and GPR109A were significantly reduced with age in both adipocyte depots.

Conclusions: Overall, gene profiling reveals a unique phenotype for primary bone marrow adipocytes characterized by low adipose-specific gene expression and high expression of inflammatory response genes. Bone marrow and epididymal adipocytes share a common pathway in response to aging in mice, but age has a greater impact on global gene expression in epididymal than in bone marrow adipocytes. Genes that are differentially expressed at greater levels in the bone marrow are highly regulated with age.

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Quantitative analysis of bone marrow adipocytes. A. The top panels are images of the distal femurs from 6-month-old and 14-month-old C57BL6/J male mice stained with H&E (magnification ×5). The bottom panels are sections stained with H&E showing the numerous large adipocytes in bone marrow from distal femurs (magnification ×20). B. Area of bone marrow fat infiltration as a % of total area. C. Numbers of bone marrow fat cells (numbers/mm2 bone marrow). Fields were taken from distal femur sections of 6-month-old and 14-month-old mice and calculated using ImagePro software.
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Figure 1: Quantitative analysis of bone marrow adipocytes. A. The top panels are images of the distal femurs from 6-month-old and 14-month-old C57BL6/J male mice stained with H&E (magnification ×5). The bottom panels are sections stained with H&E showing the numerous large adipocytes in bone marrow from distal femurs (magnification ×20). B. Area of bone marrow fat infiltration as a % of total area. C. Numbers of bone marrow fat cells (numbers/mm2 bone marrow). Fields were taken from distal femur sections of 6-month-old and 14-month-old mice and calculated using ImagePro software.

Mentions: Table 1 depicts several metabolic and biochemical parameters of the mice included in these studies. The metabolic parameters showed the expected negative effects of age on mice. Thus, there was a significant increase in weight and serum insulin and glucose levels in older mice compared to 6 month old animals. These negative metabolic effects of age were accompanied with significant increases in fat infiltration into bone marrow, as shown in Figure 1. Similarly, there were significant increases in leptin and adiponectin with age along with a significant decrease in circulating osteocalcin with age, whereas resistin, RANKL (receptor activator for nuclear factor κB ligand) and osteoprotegerin showed no changes with age.


Characterization of age-related gene expression profiling in bone marrow and epididymal adipocytes.

Liu LF, Shen WJ, Ueno M, Patel S, Kraemer FB - BMC Genomics (2011)

Quantitative analysis of bone marrow adipocytes. A. The top panels are images of the distal femurs from 6-month-old and 14-month-old C57BL6/J male mice stained with H&E (magnification ×5). The bottom panels are sections stained with H&E showing the numerous large adipocytes in bone marrow from distal femurs (magnification ×20). B. Area of bone marrow fat infiltration as a % of total area. C. Numbers of bone marrow fat cells (numbers/mm2 bone marrow). Fields were taken from distal femur sections of 6-month-old and 14-month-old mice and calculated using ImagePro software.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113784&req=5

Figure 1: Quantitative analysis of bone marrow adipocytes. A. The top panels are images of the distal femurs from 6-month-old and 14-month-old C57BL6/J male mice stained with H&E (magnification ×5). The bottom panels are sections stained with H&E showing the numerous large adipocytes in bone marrow from distal femurs (magnification ×20). B. Area of bone marrow fat infiltration as a % of total area. C. Numbers of bone marrow fat cells (numbers/mm2 bone marrow). Fields were taken from distal femur sections of 6-month-old and 14-month-old mice and calculated using ImagePro software.
Mentions: Table 1 depicts several metabolic and biochemical parameters of the mice included in these studies. The metabolic parameters showed the expected negative effects of age on mice. Thus, there was a significant increase in weight and serum insulin and glucose levels in older mice compared to 6 month old animals. These negative metabolic effects of age were accompanied with significant increases in fat infiltration into bone marrow, as shown in Figure 1. Similarly, there were significant increases in leptin and adiponectin with age along with a significant decrease in circulating osteocalcin with age, whereas resistin, RANKL (receptor activator for nuclear factor κB ligand) and osteoprotegerin showed no changes with age.

Bottom Line: Age had a substantial effect on genes associated with mitochondria function and inflammation in bone marrow adipocytes.Twenty seven genes were significantly changed with age in both adipocyte depots.Among these genes, IL6 and GPR109A were significantly reduced with age in both adipocyte depots.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Endocrinology, Stanford University, CA 94305-5103, USA.

ABSTRACT

Background: While an increase in bone marrow adiposity is associated with age-related bone disease, the function of bone marrow adipocytes has not been studied. The aim of this study was to characterize and compare the age-related gene expression profiles in bone marrow adipocytes and epididymal adipocytes.

Results: A total of 3918 (13.7%) genes were differentially expressed in bone marrow adipocytes compared to epididymal adipocytes. Bone marrow adipocytes revealed a distinct gene profile with low expression of adipocyte-specific genes peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid binding protein 4 (FABP4), perilipin (Plin1), adipsin (CFD) and high expression of genes associated with early adipocyte differentiation (CCAAT/enhancer binding protein beta (C/EBPβ), regulator of G-protein signaling 2 (RGS2). In addition, a number of genes including secreted frizzled related protein 4 (SFRP4), tumor necrosis factor α (TNFα), transforming growth factor beta 1(TGFβ1), G-protein coupled receptor 109A (GPR109A) and interleukin 6 (IL-6), that could affect adipose-derived signaling to bone are markedly increased in bone marrow adipocytes. Age had a substantial effect on genes associated with mitochondria function and inflammation in bone marrow adipocytes. Twenty seven genes were significantly changed with age in both adipocyte depots. Among these genes, IL6 and GPR109A were significantly reduced with age in both adipocyte depots.

Conclusions: Overall, gene profiling reveals a unique phenotype for primary bone marrow adipocytes characterized by low adipose-specific gene expression and high expression of inflammatory response genes. Bone marrow and epididymal adipocytes share a common pathway in response to aging in mice, but age has a greater impact on global gene expression in epididymal than in bone marrow adipocytes. Genes that are differentially expressed at greater levels in the bone marrow are highly regulated with age.

Show MeSH
Related in: MedlinePlus