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Effect of chemokine receptors CCR7 on disseminated behavior of human T cell lymphoma: clinical and experimental study.

Yang J, Wang S, Zhao G, Sun B - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: The transcript and expression levels of CCR7 were significantly higher in Hut78 than that of Jurkat cell line.The positive association between CCL21 concentration and invasiveness was found. 3) The stronger transcript and expression of PI(3)K, Akt and p- Akt were also observed in Hut78 than in Jurkat cell line.High CCR7 expression in T-NHL cells is significantly associated with lymphatic and distant dissemination as well as with tumor cell migration and invasion in vitro.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tianjin Medical University, Tianjin, China. kesiking@163.com

ABSTRACT

Background: The expression of chemokine receptors CCR7 has been studied in relation to tumor dissemination and poor prognosis in a limited number of cancers. No such studies have been done on CCR7 expression in non-Hodgkin's lymphoma (T-NHL). Our aim in this paper is to investigate the association between CCR7 expression and progression and prognosis of T-NHL.

Methods: 1) Analysis of clinical data: The specimens were obtained from 41 patients with T-NHL and 19 patients with lymphoid hyperplasia. Their corresponding clinicopathologic data were also collected. The expression levels of CCR7, MMP-2, and MMP-9 were examined by immunohistochemical staining. 2) Human T-NHL cell lines Hut 78 (cutaneous T-cell lymphoma) and Jurkat (adult T-cell leukemia/lymphoma) were cultured. The invasiveness of the two cell lines were measured with a Transwell invasion assay, and then used to study the effects of chemokine receptors on T-NHL invasion and the underlying molecular mechanism. The transcript and expression of CCR7 were evaluated using RT-PCR and western blotting.

Results: 1) The higher CCR7 and MMP-9 expression ratios were significantly associated with multiple lesions and higher stage III/IV. Moreover, a positive correlation was observed between CCR7 and MMP-9 expression. 2) The Hut 78 cell line was more invasive than the Jurkat cells in the Transwell invasion assay. The transcript and expression levels of CCR7 were significantly higher in Hut78 than that of Jurkat cell line. The T-NHL cell lines were co-cultured with chemokine CCL21 which increased the invasiveness of T-NHL cell. The positive association between CCL21 concentration and invasiveness was found. 3) The stronger transcript and expression of PI(3)K, Akt and p- Akt were also observed in Hut78 than in Jurkat cell line.

Conclusions: High CCR7 expression in T-NHL cells is significantly associated with lymphatic and distant dissemination as well as with tumor cell migration and invasion in vitro. Its underlying mechanism probably involves the PI(3)K/Akt signal pathway.

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Related in: MedlinePlus

The expression of Akt mRNA, Akt protein and p-Akt protein in Jurkat and Hut cells after CCL21 co-culture in vitro. RT-PCR amplication and Western Blot analysis of the two cell lines under the different concentration of CCL21. β-actin is positive control in RT-PCR amplication and GAPDH is positive control in Western Blot analysis. The relative grey scale of Akt mRNA, Akt protein and p-Akt protein in Hut cell were all higher than that in Jurkat cell with corresponding concentration of CCL21.
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Figure 4: The expression of Akt mRNA, Akt protein and p-Akt protein in Jurkat and Hut cells after CCL21 co-culture in vitro. RT-PCR amplication and Western Blot analysis of the two cell lines under the different concentration of CCL21. β-actin is positive control in RT-PCR amplication and GAPDH is positive control in Western Blot analysis. The relative grey scale of Akt mRNA, Akt protein and p-Akt protein in Hut cell were all higher than that in Jurkat cell with corresponding concentration of CCL21.

Mentions: The relative PI3K mRNA expression levels in all concentration groups were higher than that in the control group (P < 0.01). The relative PI3K mRNA expression levels of the Jurkat cells in the S100 and S200 groups were both higher than that in the S50 group. The expression in the S200 group was lower than that in the S100 group (P < 0.05). For the Hut 78 cells, there were no significant differences in relative expression levels in all three concentration groups. The relative expression levels in the control and S200 groups were both higher than that in the Jurkat cells. The relative expression levels had no significant differences between Hut 78 and Jurkat cells in S50 and S100 groups.


Effect of chemokine receptors CCR7 on disseminated behavior of human T cell lymphoma: clinical and experimental study.

Yang J, Wang S, Zhao G, Sun B - J. Exp. Clin. Cancer Res. (2011)

The expression of Akt mRNA, Akt protein and p-Akt protein in Jurkat and Hut cells after CCL21 co-culture in vitro. RT-PCR amplication and Western Blot analysis of the two cell lines under the different concentration of CCL21. β-actin is positive control in RT-PCR amplication and GAPDH is positive control in Western Blot analysis. The relative grey scale of Akt mRNA, Akt protein and p-Akt protein in Hut cell were all higher than that in Jurkat cell with corresponding concentration of CCL21.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113745&req=5

Figure 4: The expression of Akt mRNA, Akt protein and p-Akt protein in Jurkat and Hut cells after CCL21 co-culture in vitro. RT-PCR amplication and Western Blot analysis of the two cell lines under the different concentration of CCL21. β-actin is positive control in RT-PCR amplication and GAPDH is positive control in Western Blot analysis. The relative grey scale of Akt mRNA, Akt protein and p-Akt protein in Hut cell were all higher than that in Jurkat cell with corresponding concentration of CCL21.
Mentions: The relative PI3K mRNA expression levels in all concentration groups were higher than that in the control group (P < 0.01). The relative PI3K mRNA expression levels of the Jurkat cells in the S100 and S200 groups were both higher than that in the S50 group. The expression in the S200 group was lower than that in the S100 group (P < 0.05). For the Hut 78 cells, there were no significant differences in relative expression levels in all three concentration groups. The relative expression levels in the control and S200 groups were both higher than that in the Jurkat cells. The relative expression levels had no significant differences between Hut 78 and Jurkat cells in S50 and S100 groups.

Bottom Line: The transcript and expression levels of CCR7 were significantly higher in Hut78 than that of Jurkat cell line.The positive association between CCL21 concentration and invasiveness was found. 3) The stronger transcript and expression of PI(3)K, Akt and p- Akt were also observed in Hut78 than in Jurkat cell line.High CCR7 expression in T-NHL cells is significantly associated with lymphatic and distant dissemination as well as with tumor cell migration and invasion in vitro.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Tianjin Medical University, Tianjin, China. kesiking@163.com

ABSTRACT

Background: The expression of chemokine receptors CCR7 has been studied in relation to tumor dissemination and poor prognosis in a limited number of cancers. No such studies have been done on CCR7 expression in non-Hodgkin's lymphoma (T-NHL). Our aim in this paper is to investigate the association between CCR7 expression and progression and prognosis of T-NHL.

Methods: 1) Analysis of clinical data: The specimens were obtained from 41 patients with T-NHL and 19 patients with lymphoid hyperplasia. Their corresponding clinicopathologic data were also collected. The expression levels of CCR7, MMP-2, and MMP-9 were examined by immunohistochemical staining. 2) Human T-NHL cell lines Hut 78 (cutaneous T-cell lymphoma) and Jurkat (adult T-cell leukemia/lymphoma) were cultured. The invasiveness of the two cell lines were measured with a Transwell invasion assay, and then used to study the effects of chemokine receptors on T-NHL invasion and the underlying molecular mechanism. The transcript and expression of CCR7 were evaluated using RT-PCR and western blotting.

Results: 1) The higher CCR7 and MMP-9 expression ratios were significantly associated with multiple lesions and higher stage III/IV. Moreover, a positive correlation was observed between CCR7 and MMP-9 expression. 2) The Hut 78 cell line was more invasive than the Jurkat cells in the Transwell invasion assay. The transcript and expression levels of CCR7 were significantly higher in Hut78 than that of Jurkat cell line. The T-NHL cell lines were co-cultured with chemokine CCL21 which increased the invasiveness of T-NHL cell. The positive association between CCL21 concentration and invasiveness was found. 3) The stronger transcript and expression of PI(3)K, Akt and p- Akt were also observed in Hut78 than in Jurkat cell line.

Conclusions: High CCR7 expression in T-NHL cells is significantly associated with lymphatic and distant dissemination as well as with tumor cell migration and invasion in vitro. Its underlying mechanism probably involves the PI(3)K/Akt signal pathway.

Show MeSH
Related in: MedlinePlus