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Tumor necrosis factor-α enhances hyperbaric oxygen-induced visfatin expression via JNK pathway in human coronary arterial endothelial cells.

Wang BW, Lin CM, Wu GJ, Shyu KG - J. Biomed. Sci. (2011)

Bottom Line: Visfatin, a adipocytokine with insulin-mimetic effect, plays a role in endothelial angiogenesis.However, the molecular mechanism of beneficial effects of HBO is poorly understood.HBO significantly increased secretion of TNF-α from cultured human CAECs.

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Affiliation: Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, and School of Medicine, Fu-Jen Catholic University, New Taipei City, Taipei, Taiwan.

ABSTRACT

Background: Visfatin, a adipocytokine with insulin-mimetic effect, plays a role in endothelial angiogenesis. Hyperbaric oxygen (HBO) has been used in medical practice. However, the molecular mechanism of beneficial effects of HBO is poorly understood. We sought to investigate the cellular and molecular mechanisms of regulation of visfatin by HBO in human coronary arterial endothelial cells (CAECs).

Methods: Human CAECs were exposed to 2.5 atmosphere absolute (ATA) of oxygen in a hyperbaric chamber. Western blot, real-time polymerase chain reaction, and promoter activity assay were performed. In vitro glucose uptake and tube formation was detected.

Results: Visfatin protein (2.55-fold) and mRNA (2.53-fold) expression were significantly increased after exposure to 2.5 ATA HBO for 4 to 6 h. Addition of SP600125 and JNK siRNA 30 min before HBO inhibited the induction of visfatin protein. HBO also significantly increased DNA-protein binding activity of AP-1 and visfatin promoter activity. Addition of SP600125 and TNF-α monoclonal antibody 30 min before HBO abolished the DNA-protein binding activity and visfatin promoter activity induced by HBO. HBO significantly increased secretion of TNF-α from cultured human CAECs. Exogenous addition of TNF-α significantly increased visfatin protein expression while TNF-α antibody and TNF-α receptor antibody blocked the induction of visfatin protein expression induced by HBO. HBO increased glucose uptake in human CAECs as HBO and visfatin siRNA and TNF-α antibody attenuated the glucose uptake induced by HBO. HBO significantly increased the tube formation of human CAECs while visfatin siRNA, TNF-α antibody inhibited the tube formation induced by HBO.

Conclusions: HBO activates visfatin expression in cultured human CAECs. HBO-induced visfatin is mediated by TNF-α and at least in part through JNK pathway.

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Effect of HBO and recombinant visfatin on glucose uptake in human CAECs. Glucose uptake was measured in human CAECs treated for 90 min with 100 ng/ml recombinant visfatin with or without siRNA, TNF-α, or TNF-α antibody (Ab). *P < 0.01 vs. control; #P < 0.001 vs. HBO. Data are from 4 independent experiments.
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Figure 7: Effect of HBO and recombinant visfatin on glucose uptake in human CAECs. Glucose uptake was measured in human CAECs treated for 90 min with 100 ng/ml recombinant visfatin with or without siRNA, TNF-α, or TNF-α antibody (Ab). *P < 0.01 vs. control; #P < 0.001 vs. HBO. Data are from 4 independent experiments.

Mentions: HBO and recombinant human visfatin at 100 ng/ml significantly increased glucose uptake at various periods of incubation as compared to control human CAECs without treatment (Figure 7). The glucose uptake in HBO-treated cells was similar to that in exogenous addition of visfatin and TNF-α. Addition of visfatin siRNA or TNF-α antibody before HBO treatment attenuated the glucose uptake to baseline levels.


Tumor necrosis factor-α enhances hyperbaric oxygen-induced visfatin expression via JNK pathway in human coronary arterial endothelial cells.

Wang BW, Lin CM, Wu GJ, Shyu KG - J. Biomed. Sci. (2011)

Effect of HBO and recombinant visfatin on glucose uptake in human CAECs. Glucose uptake was measured in human CAECs treated for 90 min with 100 ng/ml recombinant visfatin with or without siRNA, TNF-α, or TNF-α antibody (Ab). *P < 0.01 vs. control; #P < 0.001 vs. HBO. Data are from 4 independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3113732&req=5

Figure 7: Effect of HBO and recombinant visfatin on glucose uptake in human CAECs. Glucose uptake was measured in human CAECs treated for 90 min with 100 ng/ml recombinant visfatin with or without siRNA, TNF-α, or TNF-α antibody (Ab). *P < 0.01 vs. control; #P < 0.001 vs. HBO. Data are from 4 independent experiments.
Mentions: HBO and recombinant human visfatin at 100 ng/ml significantly increased glucose uptake at various periods of incubation as compared to control human CAECs without treatment (Figure 7). The glucose uptake in HBO-treated cells was similar to that in exogenous addition of visfatin and TNF-α. Addition of visfatin siRNA or TNF-α antibody before HBO treatment attenuated the glucose uptake to baseline levels.

Bottom Line: Visfatin, a adipocytokine with insulin-mimetic effect, plays a role in endothelial angiogenesis.However, the molecular mechanism of beneficial effects of HBO is poorly understood.HBO significantly increased secretion of TNF-α from cultured human CAECs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, and School of Medicine, Fu-Jen Catholic University, New Taipei City, Taipei, Taiwan.

ABSTRACT

Background: Visfatin, a adipocytokine with insulin-mimetic effect, plays a role in endothelial angiogenesis. Hyperbaric oxygen (HBO) has been used in medical practice. However, the molecular mechanism of beneficial effects of HBO is poorly understood. We sought to investigate the cellular and molecular mechanisms of regulation of visfatin by HBO in human coronary arterial endothelial cells (CAECs).

Methods: Human CAECs were exposed to 2.5 atmosphere absolute (ATA) of oxygen in a hyperbaric chamber. Western blot, real-time polymerase chain reaction, and promoter activity assay were performed. In vitro glucose uptake and tube formation was detected.

Results: Visfatin protein (2.55-fold) and mRNA (2.53-fold) expression were significantly increased after exposure to 2.5 ATA HBO for 4 to 6 h. Addition of SP600125 and JNK siRNA 30 min before HBO inhibited the induction of visfatin protein. HBO also significantly increased DNA-protein binding activity of AP-1 and visfatin promoter activity. Addition of SP600125 and TNF-α monoclonal antibody 30 min before HBO abolished the DNA-protein binding activity and visfatin promoter activity induced by HBO. HBO significantly increased secretion of TNF-α from cultured human CAECs. Exogenous addition of TNF-α significantly increased visfatin protein expression while TNF-α antibody and TNF-α receptor antibody blocked the induction of visfatin protein expression induced by HBO. HBO increased glucose uptake in human CAECs as HBO and visfatin siRNA and TNF-α antibody attenuated the glucose uptake induced by HBO. HBO significantly increased the tube formation of human CAECs while visfatin siRNA, TNF-α antibody inhibited the tube formation induced by HBO.

Conclusions: HBO activates visfatin expression in cultured human CAECs. HBO-induced visfatin is mediated by TNF-α and at least in part through JNK pathway.

Show MeSH
Related in: MedlinePlus