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Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic Wistar-Kyoto rats and spontaneously hypertensive rats: effects of antioxidant (honey) treatment.

Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KN, Salleh MS, Gurtu S - Int J Mol Sci (2011)

Bottom Line: In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR.Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY.It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; E-Mails: sbsamrah@kb.usm.my (S.A.S.); msuhaimi@kb.usm.my (M.S.A.W.).

ABSTRACT
Oxidative stress is implicated in the pathogenesis and/or complications of hypertension and/or diabetes mellitus. A combination of these disorders increases the risk of developing cardiovascular events. This study investigated the effects of streptozotocin (60 mg/kg; ip)-induced diabetes on blood pressure, oxidative stress and effects of honey on these parameters in the kidneys of streptozotocin-induced diabetic Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Diabetic WKY and SHR were randomized into four groups and received distilled water (0.5 mL) and honey (1.0 g/kg) orally once daily for three weeks. Control SHR had reduced malondialdehyde (MDA) and increased systolic blood pressure (SBP), catalase (CAT) activity, and total antioxidant status (TAS). SBP, activities of glutathione peroxidase (GPx) and glutathione reductase (GR) were elevated while TAS was reduced in diabetic WKY. In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR. Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY. It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR. These data suggest that differences in types, severity, and complications of diseases as well as strains may influence responses to blood pressure and oxidative stress.

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Effect of honey on total antioxidant status (TAS) in kidney of streptozotocin induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05, ** p < 0.01 versus C-WKY; ‡‡‡ p < 0.001 versus C-SHR; §§p < 0.01 versus D-SHR.
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f7-ijms-12-01888: Effect of honey on total antioxidant status (TAS) in kidney of streptozotocin induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05, ** p < 0.01 versus C-WKY; ‡‡‡ p < 0.001 versus C-SHR; §§p < 0.01 versus D-SHR.

Mentions: Figure 7 shows the total antioxidant status (TAS) in control and STZ-induced diabetic WKY and SHR. Control SHR had significantly (p < 0.05) higher TAS than did control WKY. TAS was significantly reduced in diabetic WKY and diabetic SHR (p < 0.01 and p < 0.001, respectively). Treatment with honey significantly (p < 0.01) increased TAS in diabetic SHR but not in diabetic WKY. Figure 8 shows the malondialdehyde (MDA) levels in control and STZ-induced diabetic WKY and SHR. Control SHR had significantly (p < 0.01) lower MDA levels than did control WKY. MDA levels were much lower (though not statistically significant) in the remaining SHR groups compared to corresponding WKY. Treatment with honey further reduced MDA levels but not significantly in both diabetic WKY and diabetic SHR.


Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic Wistar-Kyoto rats and spontaneously hypertensive rats: effects of antioxidant (honey) treatment.

Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KN, Salleh MS, Gurtu S - Int J Mol Sci (2011)

Effect of honey on total antioxidant status (TAS) in kidney of streptozotocin induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05, ** p < 0.01 versus C-WKY; ‡‡‡ p < 0.001 versus C-SHR; §§p < 0.01 versus D-SHR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111640&req=5

f7-ijms-12-01888: Effect of honey on total antioxidant status (TAS) in kidney of streptozotocin induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05, ** p < 0.01 versus C-WKY; ‡‡‡ p < 0.001 versus C-SHR; §§p < 0.01 versus D-SHR.
Mentions: Figure 7 shows the total antioxidant status (TAS) in control and STZ-induced diabetic WKY and SHR. Control SHR had significantly (p < 0.05) higher TAS than did control WKY. TAS was significantly reduced in diabetic WKY and diabetic SHR (p < 0.01 and p < 0.001, respectively). Treatment with honey significantly (p < 0.01) increased TAS in diabetic SHR but not in diabetic WKY. Figure 8 shows the malondialdehyde (MDA) levels in control and STZ-induced diabetic WKY and SHR. Control SHR had significantly (p < 0.01) lower MDA levels than did control WKY. MDA levels were much lower (though not statistically significant) in the remaining SHR groups compared to corresponding WKY. Treatment with honey further reduced MDA levels but not significantly in both diabetic WKY and diabetic SHR.

Bottom Line: In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR.Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY.It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; E-Mails: sbsamrah@kb.usm.my (S.A.S.); msuhaimi@kb.usm.my (M.S.A.W.).

ABSTRACT
Oxidative stress is implicated in the pathogenesis and/or complications of hypertension and/or diabetes mellitus. A combination of these disorders increases the risk of developing cardiovascular events. This study investigated the effects of streptozotocin (60 mg/kg; ip)-induced diabetes on blood pressure, oxidative stress and effects of honey on these parameters in the kidneys of streptozotocin-induced diabetic Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Diabetic WKY and SHR were randomized into four groups and received distilled water (0.5 mL) and honey (1.0 g/kg) orally once daily for three weeks. Control SHR had reduced malondialdehyde (MDA) and increased systolic blood pressure (SBP), catalase (CAT) activity, and total antioxidant status (TAS). SBP, activities of glutathione peroxidase (GPx) and glutathione reductase (GR) were elevated while TAS was reduced in diabetic WKY. In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR. Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY. It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR. These data suggest that differences in types, severity, and complications of diseases as well as strains may influence responses to blood pressure and oxidative stress.

Show MeSH
Related in: MedlinePlus