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Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic Wistar-Kyoto rats and spontaneously hypertensive rats: effects of antioxidant (honey) treatment.

Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KN, Salleh MS, Gurtu S - Int J Mol Sci (2011)

Bottom Line: In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR.Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY.It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; E-Mails: sbsamrah@kb.usm.my (S.A.S.); msuhaimi@kb.usm.my (M.S.A.W.).

ABSTRACT
Oxidative stress is implicated in the pathogenesis and/or complications of hypertension and/or diabetes mellitus. A combination of these disorders increases the risk of developing cardiovascular events. This study investigated the effects of streptozotocin (60 mg/kg; ip)-induced diabetes on blood pressure, oxidative stress and effects of honey on these parameters in the kidneys of streptozotocin-induced diabetic Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Diabetic WKY and SHR were randomized into four groups and received distilled water (0.5 mL) and honey (1.0 g/kg) orally once daily for three weeks. Control SHR had reduced malondialdehyde (MDA) and increased systolic blood pressure (SBP), catalase (CAT) activity, and total antioxidant status (TAS). SBP, activities of glutathione peroxidase (GPx) and glutathione reductase (GR) were elevated while TAS was reduced in diabetic WKY. In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR. Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY. It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR. These data suggest that differences in types, severity, and complications of diseases as well as strains may influence responses to blood pressure and oxidative stress.

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Effect of honey on catalase activity in kidney of streptozotocin-induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05 versus C-WKY; ‡ p < 0.05 versus C-SHR.
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f4-ijms-12-01888: Effect of honey on catalase activity in kidney of streptozotocin-induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05 versus C-WKY; ‡ p < 0.05 versus C-SHR.

Mentions: Figure 4 shows the catalase (CAT) activity in control and STZ-induced diabetic WKY and SHR. Control SHR had significantly (p < 0.05) higher CAT activity compared to control WKY. With STZ-induced diabetes, CAT activity was reduced in both WKY and SHR. The reduced CAT activity was more pronounced in diabetic SHR than in diabetic WKY. Treatment of diabetic WKY and diabetic SHR with honey did not restore CAT activity. Figure 5 shows the GPx activity in control and STZ-induced diabetic WKY and SHR. GPx activity was similar in the control WKY and control SHR. GPx activity was significantly (p < 0.05) elevated in diabetic WKY while it was significantly (p < 0.05) reduced in diabetic SHR. Treatment with honey significantly (p < 0.05) restored GPx activity in both diabetic WKY and diabetic SHR. Figure 6 shows that GR activity was increased though not significantly in diabetic WKY while it was significantly (p < 0.05) reduced in diabetic SHR. Treatment with honey significantly (p < 0.05) reduced GR activity in diabetic WKY while it significantly (p < 0.05) increased GR activity in diabetic SHR. Activities of Superoxide Dismutase (SOD) and Glutathione-S-Transferase (GST) remained the same in the all the groups (data not shown).


Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic Wistar-Kyoto rats and spontaneously hypertensive rats: effects of antioxidant (honey) treatment.

Erejuwa OO, Sulaiman SA, Wahab MS, Sirajudeen KN, Salleh MS, Gurtu S - Int J Mol Sci (2011)

Effect of honey on catalase activity in kidney of streptozotocin-induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05 versus C-WKY; ‡ p < 0.05 versus C-SHR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111640&req=5

f4-ijms-12-01888: Effect of honey on catalase activity in kidney of streptozotocin-induced diabetic WKY and SHR. Each group consisted of five to seven animals. Data are expressed as mean ± SEM. WKY, Wistar-Kyoto rats; SHR, spontaneously hypertensive rats; C-WKY, control WKY; C-SHR, control SHR; D-WKY, diabetic WKY; D-SHR, diabetic SHR; DH-WKY, diabetic WKY + honey; DH-SHR, diabetic SHR + honey. * p < 0.05 versus C-WKY; ‡ p < 0.05 versus C-SHR.
Mentions: Figure 4 shows the catalase (CAT) activity in control and STZ-induced diabetic WKY and SHR. Control SHR had significantly (p < 0.05) higher CAT activity compared to control WKY. With STZ-induced diabetes, CAT activity was reduced in both WKY and SHR. The reduced CAT activity was more pronounced in diabetic SHR than in diabetic WKY. Treatment of diabetic WKY and diabetic SHR with honey did not restore CAT activity. Figure 5 shows the GPx activity in control and STZ-induced diabetic WKY and SHR. GPx activity was similar in the control WKY and control SHR. GPx activity was significantly (p < 0.05) elevated in diabetic WKY while it was significantly (p < 0.05) reduced in diabetic SHR. Treatment with honey significantly (p < 0.05) restored GPx activity in both diabetic WKY and diabetic SHR. Figure 6 shows that GR activity was increased though not significantly in diabetic WKY while it was significantly (p < 0.05) reduced in diabetic SHR. Treatment with honey significantly (p < 0.05) reduced GR activity in diabetic WKY while it significantly (p < 0.05) increased GR activity in diabetic SHR. Activities of Superoxide Dismutase (SOD) and Glutathione-S-Transferase (GST) remained the same in the all the groups (data not shown).

Bottom Line: In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR.Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY.It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; E-Mails: sbsamrah@kb.usm.my (S.A.S.); msuhaimi@kb.usm.my (M.S.A.W.).

ABSTRACT
Oxidative stress is implicated in the pathogenesis and/or complications of hypertension and/or diabetes mellitus. A combination of these disorders increases the risk of developing cardiovascular events. This study investigated the effects of streptozotocin (60 mg/kg; ip)-induced diabetes on blood pressure, oxidative stress and effects of honey on these parameters in the kidneys of streptozotocin-induced diabetic Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Diabetic WKY and SHR were randomized into four groups and received distilled water (0.5 mL) and honey (1.0 g/kg) orally once daily for three weeks. Control SHR had reduced malondialdehyde (MDA) and increased systolic blood pressure (SBP), catalase (CAT) activity, and total antioxidant status (TAS). SBP, activities of glutathione peroxidase (GPx) and glutathione reductase (GR) were elevated while TAS was reduced in diabetic WKY. In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR. Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY. It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR. These data suggest that differences in types, severity, and complications of diseases as well as strains may influence responses to blood pressure and oxidative stress.

Show MeSH
Related in: MedlinePlus