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Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

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Effect of different PAH concentrations on the growth kinetics of P. fluorescens DSS73 in DMB in oxygen-limited conditions. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.
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f5-ijms-12-01787: Effect of different PAH concentrations on the growth kinetics of P. fluorescens DSS73 in DMB in oxygen-limited conditions. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.

Mentions: As represented in Figure 5, P. fluorescens DSS73 growth was stunted with increasing PAH concentration. The higher the PAH concentration was, the longer the lag phase. For some growth kinetics, an important experimental error was observed especially at high PAH concentrations. This was related to the high variability of the lag phase observed on the three replicates. In Figure 6, the previous results were summarized and normalized: μmax was reported versus PAH concentration normalized by PAH solubility. It appeared that when the PAH concentration was lower than its solubility (e.g., concentration over solubility ratio lower than 1), μmax was not significantly affected by PAHs (p = 0.977 and p = 0.305 for pyrene and fluoranthene, respectively, with a t-test) except for phenanthrene (p = 0.007). When the PAH concentration was higher than the solubility limit, the maximal specific growth rate was drastically reduced with regard to a medium without contaminant. Similar trends were observed in both conditions with or without oxygen limitation.


Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Effect of different PAH concentrations on the growth kinetics of P. fluorescens DSS73 in DMB in oxygen-limited conditions. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111634&req=5

f5-ijms-12-01787: Effect of different PAH concentrations on the growth kinetics of P. fluorescens DSS73 in DMB in oxygen-limited conditions. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.
Mentions: As represented in Figure 5, P. fluorescens DSS73 growth was stunted with increasing PAH concentration. The higher the PAH concentration was, the longer the lag phase. For some growth kinetics, an important experimental error was observed especially at high PAH concentrations. This was related to the high variability of the lag phase observed on the three replicates. In Figure 6, the previous results were summarized and normalized: μmax was reported versus PAH concentration normalized by PAH solubility. It appeared that when the PAH concentration was lower than its solubility (e.g., concentration over solubility ratio lower than 1), μmax was not significantly affected by PAHs (p = 0.977 and p = 0.305 for pyrene and fluoranthene, respectively, with a t-test) except for phenanthrene (p = 0.007). When the PAH concentration was higher than the solubility limit, the maximal specific growth rate was drastically reduced with regard to a medium without contaminant. Similar trends were observed in both conditions with or without oxygen limitation.

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

Show MeSH