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Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

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Effect of PAH concentration on maximal specific growth rate of P. fluorescens DSS73 in DMB. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.
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f4-ijms-12-01787: Effect of PAH concentration on maximal specific growth rate of P. fluorescens DSS73 in DMB. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.

Mentions: As shown in Figure 4, for all the tested PAHs concentrations, P. fluorescens DSS73 was able to grow. At concentrations lower than PAHs saturation, the maximum specific growth rates, μmax, were in the same range with or without PAH, thus the presence of pollutants did not significantly modify bacterial development. However, when the growth medium was saturated by PAHs, the bacterial growth was more disturbed: μmax decreased drastically for each of the three PAHs.


Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Effect of PAH concentration on maximal specific growth rate of P. fluorescens DSS73 in DMB. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111634&req=5

f4-ijms-12-01787: Effect of PAH concentration on maximal specific growth rate of P. fluorescens DSS73 in DMB. (§: PAH concentration higher than its solubility limit). (A) pyrene; (B) fluoranthene; (C) phenanthrene. All experiments were done in triplicate.
Mentions: As shown in Figure 4, for all the tested PAHs concentrations, P. fluorescens DSS73 was able to grow. At concentrations lower than PAHs saturation, the maximum specific growth rates, μmax, were in the same range with or without PAH, thus the presence of pollutants did not significantly modify bacterial development. However, when the growth medium was saturated by PAHs, the bacterial growth was more disturbed: μmax decreased drastically for each of the three PAHs.

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

Show MeSH