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Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

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Adsorption isotherms of different PAHs at water/sand, water/silt and water/kaolin interfaces at 25 °C. (A) phenanthrene; (B) pyrene; (C) indeno[1,2,3,cd]pyrene.
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f2-ijms-12-01787: Adsorption isotherms of different PAHs at water/sand, water/silt and water/kaolin interfaces at 25 °C. (A) phenanthrene; (B) pyrene; (C) indeno[1,2,3,cd]pyrene.

Mentions: Only the adsorption of some of the PAHs is plotted in Figure 2 (for more clarity), i.e., phenanthrene, which represents a low weight PAH (Figure 2A), pyrene, which represents an intermediary PAH (Figure 2B) and indeno[1,2,3,cd]pyrene, which represents a high weight PAH (Figure 2C). The adsorption isotherms show the interfacial exchanges between water and sand, silt or kaolin, the main components of the harbor sediment. Two main trends are observed through these isotherms. Firstly, PAHs do not adsorb in the same way on the surface of the different sediment constituents. PAH adsorption on sand is markedly weaker than on kaolin, and silt presents an intermediate. Indeed, all PAH adsorption isotherms reach a saturation level for sand at approximately 0.2 mg·L−1 (1.8 μg of each PAH per gram of dry sand). This saturated value is higher on the silt (between 0.4 mg·L−1 and approximately 0.9 mg·L−1 according to tested PAH, i.e., between 3.6 and 8.1 μg·g−1). The saturation level is not reached on the kaolin surface in the studied concentration range. These results demonstrated that PAHs concentrate more on the finest particles, where the proportions exceed 9 μg·g−1 of dry kaolin. Secondly, PAH adsorption is related to its structure, i.e., the number of aromatic ring (three or four ring PAHs, respectively, in Figure 2 (A,B), or six ring PAHs in Figure 2C). Adsorption isotherms from fine kaolin particles are almost parallel to the y-axis for the very heavy PAHs (from benz[a]anthracene to indeno[1,2,3,cd]pyrene), meaning that they are so strongly adsorbed on the clay that we can really speak about “sequestration” of PAHs by this major constituent of the sediment.


Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Adsorption isotherms of different PAHs at water/sand, water/silt and water/kaolin interfaces at 25 °C. (A) phenanthrene; (B) pyrene; (C) indeno[1,2,3,cd]pyrene.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111634&req=5

f2-ijms-12-01787: Adsorption isotherms of different PAHs at water/sand, water/silt and water/kaolin interfaces at 25 °C. (A) phenanthrene; (B) pyrene; (C) indeno[1,2,3,cd]pyrene.
Mentions: Only the adsorption of some of the PAHs is plotted in Figure 2 (for more clarity), i.e., phenanthrene, which represents a low weight PAH (Figure 2A), pyrene, which represents an intermediary PAH (Figure 2B) and indeno[1,2,3,cd]pyrene, which represents a high weight PAH (Figure 2C). The adsorption isotherms show the interfacial exchanges between water and sand, silt or kaolin, the main components of the harbor sediment. Two main trends are observed through these isotherms. Firstly, PAHs do not adsorb in the same way on the surface of the different sediment constituents. PAH adsorption on sand is markedly weaker than on kaolin, and silt presents an intermediate. Indeed, all PAH adsorption isotherms reach a saturation level for sand at approximately 0.2 mg·L−1 (1.8 μg of each PAH per gram of dry sand). This saturated value is higher on the silt (between 0.4 mg·L−1 and approximately 0.9 mg·L−1 according to tested PAH, i.e., between 3.6 and 8.1 μg·g−1). The saturation level is not reached on the kaolin surface in the studied concentration range. These results demonstrated that PAHs concentrate more on the finest particles, where the proportions exceed 9 μg·g−1 of dry kaolin. Secondly, PAH adsorption is related to its structure, i.e., the number of aromatic ring (three or four ring PAHs, respectively, in Figure 2 (A,B), or six ring PAHs in Figure 2C). Adsorption isotherms from fine kaolin particles are almost parallel to the y-axis for the very heavy PAHs (from benz[a]anthracene to indeno[1,2,3,cd]pyrene), meaning that they are so strongly adsorbed on the clay that we can really speak about “sequestration” of PAHs by this major constituent of the sediment.

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

Show MeSH