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Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

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HPLC-UV chromatogram (λ = 210 nm) of the freeze dried biosurfactant produced by P. fluorescens DSS73, the amphisin and its structure.
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f1-ijms-12-01787: HPLC-UV chromatogram (λ = 210 nm) of the freeze dried biosurfactant produced by P. fluorescens DSS73, the amphisin and its structure.

Mentions: In order to verify the production of biosurfactant by P. fluorescens DSS73 grown on DMA, at 8 °C, the surface tension of the rinsing solution was evaluated at 32.1 ± 0.9 mN·m−1. Compared to the surface tension of the standard (i.e., 71.7 ± 0.3 mN·m−1), this value, which is lower than 40 mN·m−1, indicates the presence of a biosurfactant [49]. After purification and lyophilization, the purity of the biosurfactant, described earlier as amphisin [20,50] was analyzed by HPLC-UV. On the chromatogram presented in Figure 1, a single peak appeared, revealing an ultra-majority production of amphisin by P. fluorescens DSS73 and not a mixture of different CLPs.


Novel application of cyclolipopeptide amphisin: feasibility study as additive to remediate polycyclic aromatic hydrocarbon (PAH) contaminated sediments.

Groboillot A, Portet-Koltalo F, Le Derf F, Feuilloley MJ, Orange N, Poc CD - Int J Mol Sci (2011)

HPLC-UV chromatogram (λ = 210 nm) of the freeze dried biosurfactant produced by P. fluorescens DSS73, the amphisin and its structure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111634&req=5

f1-ijms-12-01787: HPLC-UV chromatogram (λ = 210 nm) of the freeze dried biosurfactant produced by P. fluorescens DSS73, the amphisin and its structure.
Mentions: In order to verify the production of biosurfactant by P. fluorescens DSS73 grown on DMA, at 8 °C, the surface tension of the rinsing solution was evaluated at 32.1 ± 0.9 mN·m−1. Compared to the surface tension of the standard (i.e., 71.7 ± 0.3 mN·m−1), this value, which is lower than 40 mN·m−1, indicates the presence of a biosurfactant [49]. After purification and lyophilization, the purity of the biosurfactant, described earlier as amphisin [20,50] was analyzed by HPLC-UV. On the chromatogram presented in Figure 1, a single peak appeared, revealing an ultra-majority production of amphisin by P. fluorescens DSS73 and not a mixture of different CLPs.

Bottom Line: Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant.DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced.Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cold Microbiology-Signals and Microenvironment, University of Rouen, EA 4312, 55 rue Saint Germain, 27000 Evreux, France; E-Mails: anne.groboillot@univ-rouen.fr (A.G.); marc.feuilloley@univ-rouen.fr (M.J.G.F.); nicole.orange@univ-rouen.fr (N.O.).

ABSTRACT
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73's growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production.

Show MeSH