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A novel stress-associated protein 'AtSAP10' from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress.

Dixit AR, Dhankher OP - PLoS ONE (2011)

Bottom Line: AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants.Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn.Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant, Soil, and Insect Sciences, and Plant Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts, United States of America.

ABSTRACT
We describe here the functional characterization of a novel AtSAP10, a member of the Stress Associated Protein (SAP) gene family, from Arabidopsis thaliana ecotype Columbia. AtSAP10 contains an A20 and AN1 zinc-finger domain at the N- and C-terminal, respectively. Arabidopsis SAP10 showed differential regulation by various abiotic stresses such as heavy metals and metalloids (Ni, Cd, Mn, Zn, and As), high and low temperatures, cold, and ABA. Overexpression of AtSAP10 in Arabidopsis conferred strong tolerance to heavy metals such as Ni, Mn, and Zn and to high temperature stress. AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants. Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn. AtSAP10 promoter-GUS fusion studies revealed a root and floral organ-specific expression of AtSAP10. Overexpression of AtSAP10-GFP fusion protein showed the localization in both nucleus and cytoplasm. Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

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Mn resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Mn resistance phenotypes and (B) Fresh shoot weight of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 1 mM MnCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.
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pone-0020921-g003: Mn resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Mn resistance phenotypes and (B) Fresh shoot weight of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 1 mM MnCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.

Mentions: At the end of a three weeks growth period on media containing 1 mM Mn, leaves from transgenic plants were dark green and fully expanded as compared to wild-type controls whose leaves were stunted and pale (Figure 3A). AtSAP10 plants had attained a 2- to 3-fold increase in shoot biomass (Figure 3B), but no significant difference in root length was observed.


A novel stress-associated protein 'AtSAP10' from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress.

Dixit AR, Dhankher OP - PLoS ONE (2011)

Mn resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Mn resistance phenotypes and (B) Fresh shoot weight of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 1 mM MnCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3111467&req=5

pone-0020921-g003: Mn resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Mn resistance phenotypes and (B) Fresh shoot weight of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 1 mM MnCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.
Mentions: At the end of a three weeks growth period on media containing 1 mM Mn, leaves from transgenic plants were dark green and fully expanded as compared to wild-type controls whose leaves were stunted and pale (Figure 3A). AtSAP10 plants had attained a 2- to 3-fold increase in shoot biomass (Figure 3B), but no significant difference in root length was observed.

Bottom Line: AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants.Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn.Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant, Soil, and Insect Sciences, and Plant Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts, United States of America.

ABSTRACT
We describe here the functional characterization of a novel AtSAP10, a member of the Stress Associated Protein (SAP) gene family, from Arabidopsis thaliana ecotype Columbia. AtSAP10 contains an A20 and AN1 zinc-finger domain at the N- and C-terminal, respectively. Arabidopsis SAP10 showed differential regulation by various abiotic stresses such as heavy metals and metalloids (Ni, Cd, Mn, Zn, and As), high and low temperatures, cold, and ABA. Overexpression of AtSAP10 in Arabidopsis conferred strong tolerance to heavy metals such as Ni, Mn, and Zn and to high temperature stress. AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants. Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn. AtSAP10 promoter-GUS fusion studies revealed a root and floral organ-specific expression of AtSAP10. Overexpression of AtSAP10-GFP fusion protein showed the localization in both nucleus and cytoplasm. Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

Show MeSH