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A novel stress-associated protein 'AtSAP10' from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress.

Dixit AR, Dhankher OP - PLoS ONE (2011)

Bottom Line: AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants.Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn.Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant, Soil, and Insect Sciences, and Plant Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts, United States of America.

ABSTRACT
We describe here the functional characterization of a novel AtSAP10, a member of the Stress Associated Protein (SAP) gene family, from Arabidopsis thaliana ecotype Columbia. AtSAP10 contains an A20 and AN1 zinc-finger domain at the N- and C-terminal, respectively. Arabidopsis SAP10 showed differential regulation by various abiotic stresses such as heavy metals and metalloids (Ni, Cd, Mn, Zn, and As), high and low temperatures, cold, and ABA. Overexpression of AtSAP10 in Arabidopsis conferred strong tolerance to heavy metals such as Ni, Mn, and Zn and to high temperature stress. AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants. Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn. AtSAP10 promoter-GUS fusion studies revealed a root and floral organ-specific expression of AtSAP10. Overexpression of AtSAP10-GFP fusion protein showed the localization in both nucleus and cytoplasm. Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

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Ni resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Ni resistance phenotypes, (B) Fresh shoot weight, and (C) root length of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 90 µM NiCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and all AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation and root length compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.
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pone-0020921-g002: Ni resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Ni resistance phenotypes, (B) Fresh shoot weight, and (C) root length of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 90 µM NiCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and all AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation and root length compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.

Mentions: After three weeks of growth, all four AtSAP10 transgenic lines of Arabidopsis showed strong tolerance to 90 µM NiCl2 as compared to wild type plants. The results of three representative lines are shown in Figure 2A. No phenotypic difference in growth was observed in either AtSAP10 or wild type plants grown on media without any metals. With 90 µM NiCl2, transgenic plants had well-developed leaves and normal roots, whereas leaves of wild-type plants were shriveled and pale and roots were stunted with minimum lateral branching. At this toxic concentration of Ni, transgenic plants attained an average 3-fold increase in shoot biomass and had significantly longer roots as compared to wild type controls (Figure 2B & C).


A novel stress-associated protein 'AtSAP10' from Arabidopsis thaliana confers tolerance to nickel, manganese, zinc, and high temperature stress.

Dixit AR, Dhankher OP - PLoS ONE (2011)

Ni resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Ni resistance phenotypes, (B) Fresh shoot weight, and (C) root length of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 90 µM NiCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and all AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation and root length compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3111467&req=5

pone-0020921-g002: Ni resistance phenotype of Arabidopsis AtSAP10 overexpression lines.(A) Ni resistance phenotypes, (B) Fresh shoot weight, and (C) root length of three transgenic lines AtSAP10–23, AtSAP10–30, and AtSAP10–42 overexpressing AtSAP10 from ACT2pt expression cassette and wild type (WT) plants grown on 90 µM NiCl2 in half-strength MS medium for three weeks. The average and standard deviation (SD) values are represented for four replicates of 12 seedlings each for WT and all AtSAP10 lines. The asterisks represent the significant difference in biomass accumulation and root length compared with wild type (WT) plants, (*) P<0.05, (**) P<0.01.
Mentions: After three weeks of growth, all four AtSAP10 transgenic lines of Arabidopsis showed strong tolerance to 90 µM NiCl2 as compared to wild type plants. The results of three representative lines are shown in Figure 2A. No phenotypic difference in growth was observed in either AtSAP10 or wild type plants grown on media without any metals. With 90 µM NiCl2, transgenic plants had well-developed leaves and normal roots, whereas leaves of wild-type plants were shriveled and pale and roots were stunted with minimum lateral branching. At this toxic concentration of Ni, transgenic plants attained an average 3-fold increase in shoot biomass and had significantly longer roots as compared to wild type controls (Figure 2B & C).

Bottom Line: AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants.Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn.Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant, Soil, and Insect Sciences, and Plant Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts, United States of America.

ABSTRACT
We describe here the functional characterization of a novel AtSAP10, a member of the Stress Associated Protein (SAP) gene family, from Arabidopsis thaliana ecotype Columbia. AtSAP10 contains an A20 and AN1 zinc-finger domain at the N- and C-terminal, respectively. Arabidopsis SAP10 showed differential regulation by various abiotic stresses such as heavy metals and metalloids (Ni, Cd, Mn, Zn, and As), high and low temperatures, cold, and ABA. Overexpression of AtSAP10 in Arabidopsis conferred strong tolerance to heavy metals such as Ni, Mn, and Zn and to high temperature stress. AtSAP10 transgenic plants under these stress conditions grew green and healthy, attained several-fold more biomass, and had longer roots as compared to wild type plants. Further, while these transgenic plants accumulated significantly greater amounts of Ni and Mn in both shoots and root tissues, there was no significant difference in the accumulation of Zn. AtSAP10 promoter-GUS fusion studies revealed a root and floral organ-specific expression of AtSAP10. Overexpression of AtSAP10-GFP fusion protein showed the localization in both nucleus and cytoplasm. Taken together, these results showed that AtSAP10 is a potentially useful candidate gene for engineering tolerance to heavy metals and to abiotic stress in cultivated plants.

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