Limits...
Communication impairments in mice lacking Shank1: reduced levels of ultrasonic vocalizations and scent marking behavior.

Wöhr M, Roullet FI, Hung AY, Sheng M, Crawley JN - PLoS ONE (2011)

Bottom Line: In adulthood, genotype affected scent marking behavior in the presence of female urinary pheromones.Adult Shank1(-/-) males deposited fewer scent marks in proximity to female urine than Shank1(+/+) males.Call emission in response to female urinary pheromones also differed between genotypes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Behavioral Neuroscience, National Institute of Mental Health, Bethesda, Maryland, United States of America. markus.woehr@staff.uni-marburg.de

ABSTRACT
Autism is a neurodevelopmental disorder with a strong genetic component. Core symptoms are abnormal reciprocal social interactions, qualitative impairments in communication, and repetitive and stereotyped patterns of behavior with restricted interests. Candidate genes for autism include the SHANK gene family, as mutations in SHANK2 and SHANK3 have been detected in several autistic individuals. SHANK genes code for a family of scaffolding proteins located in the postsynaptic density of excitatory synapses. To test the hypothesis that a mutation in SHANK1 contributes to the symptoms of autism, we evaluated Shank1(-/-) mutant mice for behavioral phenotypes with relevance to autism, focusing on social communication. Ultrasonic vocalizations and the deposition of scent marks appear to be two major modes of mouse communication. Our findings revealed evidence for low levels of ultrasonic vocalizations and scent marks in Shank1(-/-) mice as compared to wildtype Shank1(+/+) littermate controls. Shank1(-/-) pups emitted fewer vocalizations than Shank1(+/+) pups when isolated from mother and littermates. In adulthood, genotype affected scent marking behavior in the presence of female urinary pheromones. Adult Shank1(-/-) males deposited fewer scent marks in proximity to female urine than Shank1(+/+) males. Call emission in response to female urinary pheromones also differed between genotypes. Shank1(+/+) mice changed their calling pattern dependent on previous female interactions, while Shank1(-/-) mice were unaffected, indicating a failure of Shank1(-/-) males to learn from a social experience. The reduced levels of ultrasonic vocalizations and scent marking behavior in Shank1(-/-) mice are consistent with a phenotype relevant to social communication deficits in autism.

Show MeSH

Related in: MedlinePlus

Body weight in Shank1 mice.(A) Body weight in pups tested for isolation-induced ultrasonic vocalizations on postnatal day (pnd) 8. (B) Body weight in adult mice approximately 5 months of age. Black bar: Shank1+/+ wildtype littermate control mice; striped bar: Shank1+/− heterozygote mice; white bar: Shank1−/−  mutant mice. Data are presented as means ± standard errors of the mean. * p<0.050 vs. Shank1+/+; # p<0.050 vs. Shank1+/−.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3111434&req=5

pone-0020631-g007: Body weight in Shank1 mice.(A) Body weight in pups tested for isolation-induced ultrasonic vocalizations on postnatal day (pnd) 8. (B) Body weight in adult mice approximately 5 months of age. Black bar: Shank1+/+ wildtype littermate control mice; striped bar: Shank1+/− heterozygote mice; white bar: Shank1−/− mutant mice. Data are presented as means ± standard errors of the mean. * p<0.050 vs. Shank1+/+; # p<0.050 vs. Shank1+/−.

Mentions: In the mouse pups tested for USV in isolation, body weight differed between genotypes (F2,137 = 10.389, p<0.001; Fig. 7A). Shank1−/− mutant pups were lighter than Shank1+/− (p = 0.006) and Shank1+/+ littermate control pups (p<0.001), while the latter did not differ from each other (p = 0.251). Body temperature was not affected by genotype (F2,137 = 2.910, p = 0.058). Body weight and body temperature were not correlated with the number of USV emitted (r = −0.124, p = 0.148 and r = −0.130, p = 0.129, respectively) or other USV characteristics, with the exception of a very low negative correlation between body weight and total calling time (r = −0.171, p = 046; all other p-values >0.050). Sex had no effect on body weight or body temperature (all p-values >0.050). Parity affected body weight (F2,137 = 4.094, p = 0.045), but not body temperature (F2,137 = 0.001, p = 0.984). First litter mice were heavier than second litter mice.


Communication impairments in mice lacking Shank1: reduced levels of ultrasonic vocalizations and scent marking behavior.

Wöhr M, Roullet FI, Hung AY, Sheng M, Crawley JN - PLoS ONE (2011)

Body weight in Shank1 mice.(A) Body weight in pups tested for isolation-induced ultrasonic vocalizations on postnatal day (pnd) 8. (B) Body weight in adult mice approximately 5 months of age. Black bar: Shank1+/+ wildtype littermate control mice; striped bar: Shank1+/− heterozygote mice; white bar: Shank1−/−  mutant mice. Data are presented as means ± standard errors of the mean. * p<0.050 vs. Shank1+/+; # p<0.050 vs. Shank1+/−.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3111434&req=5

pone-0020631-g007: Body weight in Shank1 mice.(A) Body weight in pups tested for isolation-induced ultrasonic vocalizations on postnatal day (pnd) 8. (B) Body weight in adult mice approximately 5 months of age. Black bar: Shank1+/+ wildtype littermate control mice; striped bar: Shank1+/− heterozygote mice; white bar: Shank1−/− mutant mice. Data are presented as means ± standard errors of the mean. * p<0.050 vs. Shank1+/+; # p<0.050 vs. Shank1+/−.
Mentions: In the mouse pups tested for USV in isolation, body weight differed between genotypes (F2,137 = 10.389, p<0.001; Fig. 7A). Shank1−/− mutant pups were lighter than Shank1+/− (p = 0.006) and Shank1+/+ littermate control pups (p<0.001), while the latter did not differ from each other (p = 0.251). Body temperature was not affected by genotype (F2,137 = 2.910, p = 0.058). Body weight and body temperature were not correlated with the number of USV emitted (r = −0.124, p = 0.148 and r = −0.130, p = 0.129, respectively) or other USV characteristics, with the exception of a very low negative correlation between body weight and total calling time (r = −0.171, p = 046; all other p-values >0.050). Sex had no effect on body weight or body temperature (all p-values >0.050). Parity affected body weight (F2,137 = 4.094, p = 0.045), but not body temperature (F2,137 = 0.001, p = 0.984). First litter mice were heavier than second litter mice.

Bottom Line: In adulthood, genotype affected scent marking behavior in the presence of female urinary pheromones.Adult Shank1(-/-) males deposited fewer scent marks in proximity to female urine than Shank1(+/+) males.Call emission in response to female urinary pheromones also differed between genotypes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Behavioral Neuroscience, National Institute of Mental Health, Bethesda, Maryland, United States of America. markus.woehr@staff.uni-marburg.de

ABSTRACT
Autism is a neurodevelopmental disorder with a strong genetic component. Core symptoms are abnormal reciprocal social interactions, qualitative impairments in communication, and repetitive and stereotyped patterns of behavior with restricted interests. Candidate genes for autism include the SHANK gene family, as mutations in SHANK2 and SHANK3 have been detected in several autistic individuals. SHANK genes code for a family of scaffolding proteins located in the postsynaptic density of excitatory synapses. To test the hypothesis that a mutation in SHANK1 contributes to the symptoms of autism, we evaluated Shank1(-/-) mutant mice for behavioral phenotypes with relevance to autism, focusing on social communication. Ultrasonic vocalizations and the deposition of scent marks appear to be two major modes of mouse communication. Our findings revealed evidence for low levels of ultrasonic vocalizations and scent marks in Shank1(-/-) mice as compared to wildtype Shank1(+/+) littermate controls. Shank1(-/-) pups emitted fewer vocalizations than Shank1(+/+) pups when isolated from mother and littermates. In adulthood, genotype affected scent marking behavior in the presence of female urinary pheromones. Adult Shank1(-/-) males deposited fewer scent marks in proximity to female urine than Shank1(+/+) males. Call emission in response to female urinary pheromones also differed between genotypes. Shank1(+/+) mice changed their calling pattern dependent on previous female interactions, while Shank1(-/-) mice were unaffected, indicating a failure of Shank1(-/-) males to learn from a social experience. The reduced levels of ultrasonic vocalizations and scent marking behavior in Shank1(-/-) mice are consistent with a phenotype relevant to social communication deficits in autism.

Show MeSH
Related in: MedlinePlus