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Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

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Related in: MedlinePlus

Score values of follicular hyperplasia for all examined groups. Note the absence of significant differences between all groups and evaluation times, except for the sham operated group after six months and the control group without surgery (* = p < 0.05). MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.
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Figure 9: Score values of follicular hyperplasia for all examined groups. Note the absence of significant differences between all groups and evaluation times, except for the sham operated group after six months and the control group without surgery (* = p < 0.05). MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.

Mentions: The lymph nodes from the control group without surgery did not show any signs of follicular hyperplasia (SV 0.0). After three months implantation duration, follicular hyperplasia was found in a moderate level for all material groups (LAE442, SV 2.2; PLA and Ti, SV 2.0; MgCa0.8, SV 1.6) as well as in the sham operated group (SV 1.5). After six months, a significant decrease could only be seen in the control group without surgery (SV 0.5), a slight decrease was found in LAE442 (SV 1.9). The other material groups showed no difference over time (PLA, SV 2.0) or a slight increase (MgCa0.8, SV 1.8) (Figure 9). The increase of CD79α expression was corresponded to the presence of follicular hyperplasia in H&E-staining (Figure 3). However, as was mentioned above, there were no meaningful differences between all groups and times.


Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Score values of follicular hyperplasia for all examined groups. Note the absence of significant differences between all groups and evaluation times, except for the sham operated group after six months and the control group without surgery (* = p < 0.05). MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3111424&req=5

Figure 9: Score values of follicular hyperplasia for all examined groups. Note the absence of significant differences between all groups and evaluation times, except for the sham operated group after six months and the control group without surgery (* = p < 0.05). MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.
Mentions: The lymph nodes from the control group without surgery did not show any signs of follicular hyperplasia (SV 0.0). After three months implantation duration, follicular hyperplasia was found in a moderate level for all material groups (LAE442, SV 2.2; PLA and Ti, SV 2.0; MgCa0.8, SV 1.6) as well as in the sham operated group (SV 1.5). After six months, a significant decrease could only be seen in the control group without surgery (SV 0.5), a slight decrease was found in LAE442 (SV 1.9). The other material groups showed no difference over time (PLA, SV 2.0) or a slight increase (MgCa0.8, SV 1.8) (Figure 9). The increase of CD79α expression was corresponded to the presence of follicular hyperplasia in H&E-staining (Figure 3). However, as was mentioned above, there were no meaningful differences between all groups and times.

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

Show MeSH
Related in: MedlinePlus