Limits...
Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

Show MeSH

Related in: MedlinePlus

Score values of histiocytic apoptosis for all examined groups (* = p < 0.05). Three months: PLA and Ti showed significant higher values than MgCa, LAE, sham operated and control group; Six months: MgCa showed a distinct increase, the values for PLA, Ti, LAE and for the sham operated group decreased. MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3111424&req=5

Figure 7: Score values of histiocytic apoptosis for all examined groups (* = p < 0.05). Three months: PLA and Ti showed significant higher values than MgCa, LAE, sham operated and control group; Six months: MgCa showed a distinct increase, the values for PLA, Ti, LAE and for the sham operated group decreased. MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.

Mentions: The most distinct differences between the material groups could be found with the evaluation of histiocytic apoptosis. After three months implantation duration PLA (SV 4.0) and titanium (SV 3.5) showed significant higher values (p < 0.05) than MgCa0.8 (score 0.8), with the lowest level of apoptosis, LAE442 (score 1.8) and both control groups (sham operated SV 1.0 and control without surgery SV 0.3). After six months of implantation duration the results changed obviously. MgCa0.8 showed a distinct increase in the number of apoptotic histiocytes (score value 3.0, p < 0.05). Contrary, the values for PLA (SV 3.7), titanium (SV 2.7), LAE442 (SV 1.6) and for the sham operated group (SV 0.7) decreased. LAE442 showed clearly lower score values than all other material groups (Figure 7).


Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Score values of histiocytic apoptosis for all examined groups (* = p < 0.05). Three months: PLA and Ti showed significant higher values than MgCa, LAE, sham operated and control group; Six months: MgCa showed a distinct increase, the values for PLA, Ti, LAE and for the sham operated group decreased. MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3111424&req=5

Figure 7: Score values of histiocytic apoptosis for all examined groups (* = p < 0.05). Three months: PLA and Ti showed significant higher values than MgCa, LAE, sham operated and control group; Six months: MgCa showed a distinct increase, the values for PLA, Ti, LAE and for the sham operated group decreased. MgCa = MgCa0.8, LAE = LAE442, PLA = poly-96L/4D-lactide, Ti = TiAl6V4, sham operated = surgery without implant material, control = no surgery on the limb.
Mentions: The most distinct differences between the material groups could be found with the evaluation of histiocytic apoptosis. After three months implantation duration PLA (SV 4.0) and titanium (SV 3.5) showed significant higher values (p < 0.05) than MgCa0.8 (score 0.8), with the lowest level of apoptosis, LAE442 (score 1.8) and both control groups (sham operated SV 1.0 and control without surgery SV 0.3). After six months of implantation duration the results changed obviously. MgCa0.8 showed a distinct increase in the number of apoptotic histiocytes (score value 3.0, p < 0.05). Contrary, the values for PLA (SV 3.7), titanium (SV 2.7), LAE442 (SV 1.6) and for the sham operated group (SV 0.7) decreased. LAE442 showed clearly lower score values than all other material groups (Figure 7).

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

Show MeSH
Related in: MedlinePlus