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Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

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Physiological structure of a lymph node from a rabbit of the control group without surgery. A. Routine histological staining allows the recognition of different compartments: follicles (F), paracortical zone (P) and medullary sinuses (MS), (H&E, ×100). B. CD79α-immunostaining detects mature B-cells, predominantly located in the follicles, and plasma cells in the paracortical zone (IHC method, ×100). C. CD3-immunostaining detects T-cells, predominantly located in the paracortical zone (IHC method, ×100).
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Figure 2: Physiological structure of a lymph node from a rabbit of the control group without surgery. A. Routine histological staining allows the recognition of different compartments: follicles (F), paracortical zone (P) and medullary sinuses (MS), (H&E, ×100). B. CD79α-immunostaining detects mature B-cells, predominantly located in the follicles, and plasma cells in the paracortical zone (IHC method, ×100). C. CD3-immunostaining detects T-cells, predominantly located in the paracortical zone (IHC method, ×100).

Mentions: The control groups with surgery but without implant showed the presence of sinus histiocytosis, follicular hyperplasia and heterophilic infiltration with low score values overall and demonstrated decrease of all investigated characteristic values from three to six months. The control group without surgery had the lowest score values in all investigated parameters. Expression of CD3 and CD79α in physiological (Figure 2B, 2C) as well as in affected lymph nodes was conformed to T- and B-dependent zones (Figure 3C, 3E). CD3 was expressed in T-cells of the paracortical zone as clear membrane staining. Single T-cells were found in cortex near or within the follicles. There were no significant differences for CD3 expression in all groups and times including the control group without surgery (Figure 2C). CD79α was expressed in B-cells of the cortex, primary (Figure 2B) and secondary follicles, and rarely in medullary cords (plasma cell reaction). A few of B-cells and plasma cells were found in sinuses.


Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Physiological structure of a lymph node from a rabbit of the control group without surgery. A. Routine histological staining allows the recognition of different compartments: follicles (F), paracortical zone (P) and medullary sinuses (MS), (H&E, ×100). B. CD79α-immunostaining detects mature B-cells, predominantly located in the follicles, and plasma cells in the paracortical zone (IHC method, ×100). C. CD3-immunostaining detects T-cells, predominantly located in the paracortical zone (IHC method, ×100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3111424&req=5

Figure 2: Physiological structure of a lymph node from a rabbit of the control group without surgery. A. Routine histological staining allows the recognition of different compartments: follicles (F), paracortical zone (P) and medullary sinuses (MS), (H&E, ×100). B. CD79α-immunostaining detects mature B-cells, predominantly located in the follicles, and plasma cells in the paracortical zone (IHC method, ×100). C. CD3-immunostaining detects T-cells, predominantly located in the paracortical zone (IHC method, ×100).
Mentions: The control groups with surgery but without implant showed the presence of sinus histiocytosis, follicular hyperplasia and heterophilic infiltration with low score values overall and demonstrated decrease of all investigated characteristic values from three to six months. The control group without surgery had the lowest score values in all investigated parameters. Expression of CD3 and CD79α in physiological (Figure 2B, 2C) as well as in affected lymph nodes was conformed to T- and B-dependent zones (Figure 3C, 3E). CD3 was expressed in T-cells of the paracortical zone as clear membrane staining. Single T-cells were found in cortex near or within the follicles. There were no significant differences for CD3 expression in all groups and times including the control group without surgery (Figure 2C). CD79α was expressed in B-cells of the cortex, primary (Figure 2B) and secondary follicles, and rarely in medullary cords (plasma cell reaction). A few of B-cells and plasma cells were found in sinuses.

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

Show MeSH
Related in: MedlinePlus