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Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

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Histiocytic karyorrhexis (arrows) as sign for apoptotic changes of histiocytes, (H&E, × 1000). Histiocytes with indicated apoptotic changes were counted and evaluated in accordance to the scoring system.
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Figure 1: Histiocytic karyorrhexis (arrows) as sign for apoptotic changes of histiocytes, (H&E, × 1000). Histiocytes with indicated apoptotic changes were counted and evaluated in accordance to the scoring system.

Mentions: After a three times repeated evaluation of all sections, a specific set of the most abundant changes was established. Four of the most common characteristics (sinus histiocytosis, follicular hyperplasia, heterophilic infiltration and histiocytic apoptosis) were chosen. In H&E stained sections, heterophiles were counted. Sinus histiocytosis and follicular hyperplasia were evaluated semiquantitatively as a comparison of follicles and sinuses in lymph nodes of experimental groups with the size of appropriate compartments (follicles and sinuses respectively) of intact popliteal lymph nodes in the control group of rabbits without surgery (Table 1). The presence of B-cells, T-cells and histiocytes in H&E staining was confirmed by CD79α, CD3 and CD68 immunostaining. Apoptotic changes of histiocytes were detected in appearance of karyopyknosis, karyorrhexis (Figure 1) and, seldom, apoptotic bodies [21]. The histiocytes with indicated apoptotic changes were counted and evaluated in accordance to the scoring system (Table 1).


Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.

Bondarenko A, Hewicker-Trautwein M, Erdmann N, Angrisani N, Reifenrath J, Meyer-Lindenberg A - Biomed Eng Online (2011)

Histiocytic karyorrhexis (arrows) as sign for apoptotic changes of histiocytes, (H&E, × 1000). Histiocytes with indicated apoptotic changes were counted and evaluated in accordance to the scoring system.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3111424&req=5

Figure 1: Histiocytic karyorrhexis (arrows) as sign for apoptotic changes of histiocytes, (H&E, × 1000). Histiocytes with indicated apoptotic changes were counted and evaluated in accordance to the scoring system.
Mentions: After a three times repeated evaluation of all sections, a specific set of the most abundant changes was established. Four of the most common characteristics (sinus histiocytosis, follicular hyperplasia, heterophilic infiltration and histiocytic apoptosis) were chosen. In H&E stained sections, heterophiles were counted. Sinus histiocytosis and follicular hyperplasia were evaluated semiquantitatively as a comparison of follicles and sinuses in lymph nodes of experimental groups with the size of appropriate compartments (follicles and sinuses respectively) of intact popliteal lymph nodes in the control group of rabbits without surgery (Table 1). The presence of B-cells, T-cells and histiocytes in H&E staining was confirmed by CD79α, CD3 and CD68 immunostaining. Apoptotic changes of histiocytes were detected in appearance of karyopyknosis, karyorrhexis (Figure 1) and, seldom, apoptotic bodies [21]. The histiocytes with indicated apoptotic changes were counted and evaluated in accordance to the scoring system (Table 1).

Bottom Line: After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery.LAE442 induced even lower immunological reactions.Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, Dnipropetrovs’k State Medical Academy, str. Zhovtneva ploshcha 14, 49005 Dnipropetrovs’k, Ukraine.

ABSTRACT

Background: Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material.

Methods: The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score.

Results: The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential.

Conclusion: Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.

Show MeSH
Related in: MedlinePlus