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Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense.

Sant'anna FH, Andrade DS, Trentini DB, Weber SS, Schrank IS - BMC Microbiol. (2011)

Bottom Line: Finally, a promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium.In this work, genetic tools that can support the study of A. amazonense were described.These methods could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Campus do Vale, Porto Alegre, RS, Brazil.

ABSTRACT

Background: Azospirillum amazonense has potential to be used as agricultural inoculant since it promotes plant growth without causing pollution, unlike industrial fertilizers. Owing to this fact, the study of this species has gained interest. However, a detailed understanding of its genetics and physiology is limited by the absence of appropriate genetic tools for the study of this species.

Results: Conjugation and electrotransformation methods were established utilizing vectors with broad host-replication origins (pVS1 and pBBR1). Two genes of interest--glnK and glnB, encoding PII regulatory proteins--were isolated. Furthermore, glnK-specific A. amazonense mutants were generated utilizing the pK19MOBSACB vector system. Finally, a promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium.

Conclusion: In this work, genetic tools that can support the study of A. amazonense were described. These methods could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion.

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Physical maps of the glnK and glnB regions of A. Amazonense. Genes are represented by the large arrows; glnA, ubiH and ftsK were not completely sequenced.
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Figure 1: Physical maps of the glnK and glnB regions of A. Amazonense. Genes are represented by the large arrows; glnA, ubiH and ftsK were not completely sequenced.

Mentions: The glnK gene from A. amazonense is flanked by the aat gene in the downstream region, which codes a putative aspartate aminotransferase and the ubiH gene in the upstream region, which codes an enzyme implicated in ubiquinone biosynthesis (Figure 1). This genetic organization resembles that found in other species from the Rhodospirillales order, namely A. brasilense, Azospirillum sp. B510 and R. centenum.


Tools for genetic manipulation of the plant growth-promoting bacterium Azospirillum amazonense.

Sant'anna FH, Andrade DS, Trentini DB, Weber SS, Schrank IS - BMC Microbiol. (2011)

Physical maps of the glnK and glnB regions of A. Amazonense. Genes are represented by the large arrows; glnA, ubiH and ftsK were not completely sequenced.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3111374&req=5

Figure 1: Physical maps of the glnK and glnB regions of A. Amazonense. Genes are represented by the large arrows; glnA, ubiH and ftsK were not completely sequenced.
Mentions: The glnK gene from A. amazonense is flanked by the aat gene in the downstream region, which codes a putative aspartate aminotransferase and the ubiH gene in the upstream region, which codes an enzyme implicated in ubiquinone biosynthesis (Figure 1). This genetic organization resembles that found in other species from the Rhodospirillales order, namely A. brasilense, Azospirillum sp. B510 and R. centenum.

Bottom Line: Finally, a promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium.In this work, genetic tools that can support the study of A. amazonense were described.These methods could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Campus do Vale, Porto Alegre, RS, Brazil.

ABSTRACT

Background: Azospirillum amazonense has potential to be used as agricultural inoculant since it promotes plant growth without causing pollution, unlike industrial fertilizers. Owing to this fact, the study of this species has gained interest. However, a detailed understanding of its genetics and physiology is limited by the absence of appropriate genetic tools for the study of this species.

Results: Conjugation and electrotransformation methods were established utilizing vectors with broad host-replication origins (pVS1 and pBBR1). Two genes of interest--glnK and glnB, encoding PII regulatory proteins--were isolated. Furthermore, glnK-specific A. amazonense mutants were generated utilizing the pK19MOBSACB vector system. Finally, a promoter analysis protocol based on fluorescent protein expression was optimized to aid genetic regulation studies on this bacterium.

Conclusion: In this work, genetic tools that can support the study of A. amazonense were described. These methods could provide a better understanding of the genetic mechanisms of this species that underlie its plant growth promotion.

Show MeSH
Related in: MedlinePlus