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Fatty acid desaturase (FADS) gene polymorphisms and insulin resistance in association with serum phospholipid polyunsaturated fatty acid composition in healthy Korean men: cross-sectional study.

Kim OY, Lim HH, Yang LI, Chae JS, Lee JH - Nutr Metab (Lond) (2011)

Bottom Line: The proportion of linoleic acid (18:2ω-6, LA) was higher in the minor allele carriers of FEN1-10154G>T, rs174575C>G and rs2727270C>T than the major homozygotes, respectively.AA was also significantly lower in the rs1000778T allele carriers than the CC homozygotes.HOMA-IR positively correlated with LA and DGLA and negatively with AA/DGLA in total subjects.

View Article: PubMed Central - HTML - PubMed

Affiliation: Yonsei University Research Institute of Science for Aging, Yonsei University, Seoul, Korea. jhleeb@yonsei.ac.kr.

ABSTRACT

Background: We investigated the relationship between fatty acid desaturase (FADS) gene polymorphisms and insulin resistance (IR) in association with serum phospholipid polyunsaturated fatty acid (FA) composition in healthy Korean men.

Methods: Healthy men (n = 576, 30 ~ 79 years old) were genotyped for rs174537 near FADS1 (FEN1-10154G>T), FADS2 (rs174575C>G, rs2727270C>T), and FADS3 (rs1000778C>T) SNPs. Dietary intake, serum phospholipid FA composition and HOMA-IR were measured.

Results: Fasting insulin and HOMA-IR were significantly higher in the rs174575G allele carriers than the CC homozygotes, but lower in the rs2727270T allele carriers than the CC homozygotes. The proportion of linoleic acid (18:2ω-6, LA) was higher in the minor allele carriers of FEN1-10154G>T, rs174575C>G and rs2727270C>T than the major homozygotes, respectively. On the other hand, the proportions of dihomo-γ-linolenic acid (20:3ω-6, DGLA) and arachidonic acid (20:4ω-6, AA) in serum phospholipids were significantly lower in the minor allele carriers of FEN1-10154 G>T carriers and rs2727270C>T than the major homozygotes respectively. AA was also significantly lower in the rs1000778T allele carriers than the CC homozygotes. HOMA-IR positively correlated with LA and DGLA and negatively with AA/DGLA in total subjects. Interestingly, rs174575G allele carriers showed remarkably higher HOMA-IR than the CC homozygotes when subjects had higher proportions of DLGA (≥1.412% in total serum phospholipid FA composition) (P for interaction = 0.009) or of AA (≥4.573%) (P for interaction = 0.047).

Conclusion: HOMA-IR is associated with FADS gene cluster as well as with FA composition in serum phospholipids. Additionally, HOMA-IR may be modulated by the interaction between rs174575C>G and the proportion of DGLA or AA in serum phospholipids.

No MeSH data available.


Related in: MedlinePlus

Insulin resistance according to the proportion of dihomo-γ-linolenic acid (20:3ω-6, DGLA) or arachidonic acid (20:4ω-6, AA) in serum phospholipid and FADS gene polymorphisms. Means ± S.D. (n), §tested by log-transformed, P for interaction was tested by multiple logistic regression analysis after adjusting for age and body mass index. HOMA-IR: homeostasis model assessment of insulin resistance
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Figure 1: Insulin resistance according to the proportion of dihomo-γ-linolenic acid (20:3ω-6, DGLA) or arachidonic acid (20:4ω-6, AA) in serum phospholipid and FADS gene polymorphisms. Means ± S.D. (n), §tested by log-transformed, P for interaction was tested by multiple logistic regression analysis after adjusting for age and body mass index. HOMA-IR: homeostasis model assessment of insulin resistance

Mentions: Based on the correlation results between HOMA-IR and FA compositions mentioned above, study subjects were subdivided into two groups according to the proportion of LA, DGLA or AA in serum phospholipids: upper median (LA≥12.396%, DGLA≥1.412% and AA≥4.573%. of total FA composition in serum phospholipids) and lower median (LA < 12.396%, DGAL < 1.412% and AA < 4.573%). Then, we investigated the interaction effect of genetic variants and proportion of LA, DGLA or AA on their HOMA-IR levels (Figure 1). Interestingly, HOMA-IR was significantly higher in minor G allele carriers of rs174575C>G than the CC homozygotes, when the proportion of DGLA or AA were higher than median value (Interaction P = 0.009 and P = 0.047, respectively). However, significant interaction effect was neither observed with other SNPs (FEN1 -10154G>T, FADS2 rs2727270C>T or FADS3 rs1000778C>T) nor with LA.


Fatty acid desaturase (FADS) gene polymorphisms and insulin resistance in association with serum phospholipid polyunsaturated fatty acid composition in healthy Korean men: cross-sectional study.

Kim OY, Lim HH, Yang LI, Chae JS, Lee JH - Nutr Metab (Lond) (2011)

Insulin resistance according to the proportion of dihomo-γ-linolenic acid (20:3ω-6, DGLA) or arachidonic acid (20:4ω-6, AA) in serum phospholipid and FADS gene polymorphisms. Means ± S.D. (n), §tested by log-transformed, P for interaction was tested by multiple logistic regression analysis after adjusting for age and body mass index. HOMA-IR: homeostasis model assessment of insulin resistance
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3111337&req=5

Figure 1: Insulin resistance according to the proportion of dihomo-γ-linolenic acid (20:3ω-6, DGLA) or arachidonic acid (20:4ω-6, AA) in serum phospholipid and FADS gene polymorphisms. Means ± S.D. (n), §tested by log-transformed, P for interaction was tested by multiple logistic regression analysis after adjusting for age and body mass index. HOMA-IR: homeostasis model assessment of insulin resistance
Mentions: Based on the correlation results between HOMA-IR and FA compositions mentioned above, study subjects were subdivided into two groups according to the proportion of LA, DGLA or AA in serum phospholipids: upper median (LA≥12.396%, DGLA≥1.412% and AA≥4.573%. of total FA composition in serum phospholipids) and lower median (LA < 12.396%, DGAL < 1.412% and AA < 4.573%). Then, we investigated the interaction effect of genetic variants and proportion of LA, DGLA or AA on their HOMA-IR levels (Figure 1). Interestingly, HOMA-IR was significantly higher in minor G allele carriers of rs174575C>G than the CC homozygotes, when the proportion of DGLA or AA were higher than median value (Interaction P = 0.009 and P = 0.047, respectively). However, significant interaction effect was neither observed with other SNPs (FEN1 -10154G>T, FADS2 rs2727270C>T or FADS3 rs1000778C>T) nor with LA.

Bottom Line: The proportion of linoleic acid (18:2ω-6, LA) was higher in the minor allele carriers of FEN1-10154G>T, rs174575C>G and rs2727270C>T than the major homozygotes, respectively.AA was also significantly lower in the rs1000778T allele carriers than the CC homozygotes.HOMA-IR positively correlated with LA and DGLA and negatively with AA/DGLA in total subjects.

View Article: PubMed Central - HTML - PubMed

Affiliation: Yonsei University Research Institute of Science for Aging, Yonsei University, Seoul, Korea. jhleeb@yonsei.ac.kr.

ABSTRACT

Background: We investigated the relationship between fatty acid desaturase (FADS) gene polymorphisms and insulin resistance (IR) in association with serum phospholipid polyunsaturated fatty acid (FA) composition in healthy Korean men.

Methods: Healthy men (n = 576, 30 ~ 79 years old) were genotyped for rs174537 near FADS1 (FEN1-10154G>T), FADS2 (rs174575C>G, rs2727270C>T), and FADS3 (rs1000778C>T) SNPs. Dietary intake, serum phospholipid FA composition and HOMA-IR were measured.

Results: Fasting insulin and HOMA-IR were significantly higher in the rs174575G allele carriers than the CC homozygotes, but lower in the rs2727270T allele carriers than the CC homozygotes. The proportion of linoleic acid (18:2ω-6, LA) was higher in the minor allele carriers of FEN1-10154G>T, rs174575C>G and rs2727270C>T than the major homozygotes, respectively. On the other hand, the proportions of dihomo-γ-linolenic acid (20:3ω-6, DGLA) and arachidonic acid (20:4ω-6, AA) in serum phospholipids were significantly lower in the minor allele carriers of FEN1-10154 G>T carriers and rs2727270C>T than the major homozygotes respectively. AA was also significantly lower in the rs1000778T allele carriers than the CC homozygotes. HOMA-IR positively correlated with LA and DGLA and negatively with AA/DGLA in total subjects. Interestingly, rs174575G allele carriers showed remarkably higher HOMA-IR than the CC homozygotes when subjects had higher proportions of DLGA (≥1.412% in total serum phospholipid FA composition) (P for interaction = 0.009) or of AA (≥4.573%) (P for interaction = 0.047).

Conclusion: HOMA-IR is associated with FADS gene cluster as well as with FA composition in serum phospholipids. Additionally, HOMA-IR may be modulated by the interaction between rs174575C>G and the proportion of DGLA or AA in serum phospholipids.

No MeSH data available.


Related in: MedlinePlus