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Screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. An Antarctic survey.

Araújo LS, Kagohara E, Garcia TP, Pellizari VH, Andrade LH - Mar Drugs (2011)

Bottom Line: Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity.From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C.We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, SP 05508-900 São Paulo, Brazil.

ABSTRACT
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

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Spectrophotometric analysis of optical density (OD) of the culture of Flavobacterium sp. (A) and Arthrobacter sp. (B). Culture medium (600 mL), (RS)-1-(phenyl)ethanol (1 mmol/L), 160 rpm.
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f2-marinedrugs-09-00889: Spectrophotometric analysis of optical density (OD) of the culture of Flavobacterium sp. (A) and Arthrobacter sp. (B). Culture medium (600 mL), (RS)-1-(phenyl)ethanol (1 mmol/L), 160 rpm.

Mentions: Therefore, we decided to determine the growth curves of Flavobacterium sp. and Arthrobacter sp. at 15, 20 and 25 °C (Figure 2). By spectrophotometric analysis of optical density (OD) of bacterial culture it was evaluated the turbidity and estimated the relative concentration cell.


Screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. An Antarctic survey.

Araújo LS, Kagohara E, Garcia TP, Pellizari VH, Andrade LH - Mar Drugs (2011)

Spectrophotometric analysis of optical density (OD) of the culture of Flavobacterium sp. (A) and Arthrobacter sp. (B). Culture medium (600 mL), (RS)-1-(phenyl)ethanol (1 mmol/L), 160 rpm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111190&req=5

f2-marinedrugs-09-00889: Spectrophotometric analysis of optical density (OD) of the culture of Flavobacterium sp. (A) and Arthrobacter sp. (B). Culture medium (600 mL), (RS)-1-(phenyl)ethanol (1 mmol/L), 160 rpm.
Mentions: Therefore, we decided to determine the growth curves of Flavobacterium sp. and Arthrobacter sp. at 15, 20 and 25 °C (Figure 2). By spectrophotometric analysis of optical density (OD) of bacterial culture it was evaluated the turbidity and estimated the relative concentration cell.

Bottom Line: Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity.From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C.We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, SP 05508-900 São Paulo, Brazil.

ABSTRACT
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

Show MeSH