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Screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. An Antarctic survey.

Araújo LS, Kagohara E, Garcia TP, Pellizari VH, Andrade LH - Mar Drugs (2011)

Bottom Line: Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity.From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C.We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, SP 05508-900 São Paulo, Brazil.

ABSTRACT
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

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Analysis using GC. (a) Sample containing a mixture of (RS)-1-(4-methoxyphenyl)ethanol and 4-methoxyacetophenone. (b) Sample from oxidation reaction of (RS)-3 mediated by Arthrobacter sp. (c) Sample from oxidation reaction of (RS)-3 mediated by Flavobacterium sp.
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f1-marinedrugs-09-00889: Analysis using GC. (a) Sample containing a mixture of (RS)-1-(4-methoxyphenyl)ethanol and 4-methoxyacetophenone. (b) Sample from oxidation reaction of (RS)-3 mediated by Arthrobacter sp. (c) Sample from oxidation reaction of (RS)-3 mediated by Flavobacterium sp.

Mentions: The chromatograms from oxidation reaction of the compound (RS)-3 mediated by cells of Flavobacterium sp. and Arthrobacter sp. (Table 2, Entries 3 and 9) are shown in Figure 1, as an example of the chiral analysis using GC employed for this enzyme evaluation step.


Screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. An Antarctic survey.

Araújo LS, Kagohara E, Garcia TP, Pellizari VH, Andrade LH - Mar Drugs (2011)

Analysis using GC. (a) Sample containing a mixture of (RS)-1-(4-methoxyphenyl)ethanol and 4-methoxyacetophenone. (b) Sample from oxidation reaction of (RS)-3 mediated by Arthrobacter sp. (c) Sample from oxidation reaction of (RS)-3 mediated by Flavobacterium sp.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111190&req=5

f1-marinedrugs-09-00889: Analysis using GC. (a) Sample containing a mixture of (RS)-1-(4-methoxyphenyl)ethanol and 4-methoxyacetophenone. (b) Sample from oxidation reaction of (RS)-3 mediated by Arthrobacter sp. (c) Sample from oxidation reaction of (RS)-3 mediated by Flavobacterium sp.
Mentions: The chromatograms from oxidation reaction of the compound (RS)-3 mediated by cells of Flavobacterium sp. and Arthrobacter sp. (Table 2, Entries 3 and 9) are shown in Figure 1, as an example of the chiral analysis using GC employed for this enzyme evaluation step.

Bottom Line: Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity.From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C.We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, SP 05508-900 São Paulo, Brazil.

ABSTRACT
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph.

Show MeSH