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Volatile constituents, inorganic elements and primary screening of bioactivity of black coral cigarette holders.

Bai X, Chen Y, Chen W, Lei H, Shi G - Mar Drugs (2011)

Bottom Line: In total, 15 components were reliably identified in BC and found to be biologically active.In the bioactivity tests, the BC extract (BCE) showed a scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl radicals by phenanthroline-Fe (II) oxidation and moderate inhibition of Gram-positive microorganisms.The antioxidant and antimicrobial activities of BC, which are related to the active chemical composition, may explain the perceived benefit for cigarette smokers who use BCCHs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.

ABSTRACT
Black corals (BC) have been used for a long time in Chinese medicine, and may have some pharmaceutical functions when used as material for cigarette holders in southeast China. This study is aimed to investigate the bioactivities of volatile constituents in BC and to explore the folklore behind the use of BC cigarette holders (BCCHs). We extracted the volatile constituents of BC by supercritical fluid extraction (SFE) with carbon dioxide (CO₂-SFE), then identified and analyzed the constituents by gas chromatography-mass spectrometry (GC-MS). In total, 15 components were reliably identified in BC and found to be biologically active. These included triethyl phosphate, butylated hydroxytoluene, cedrol, n-hexadecanoic acid, squalene, and cholesterol. Meanwhile 13 inorganic elements (P, Ca, Mg, S, B, Si, Fe, Cu, Zn, Ba, etc.) were determined by inductively coupled plasma spectrometer (ICPS). In the bioactivity tests, the BC extract (BCE) showed a scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl radicals by phenanthroline-Fe (II) oxidation and moderate inhibition of Gram-positive microorganisms. The antioxidant and antimicrobial activities of BC, which are related to the active chemical composition, may explain the perceived benefit for cigarette smokers who use BCCHs.

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Related in: MedlinePlus

The total ion chromatogram of the volatile constituents from black coral by supercritical fluid with carbon dioxide (CO2-SFE).
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Related In: Results  -  Collection

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f1-marinedrugs-09-00863: The total ion chromatogram of the volatile constituents from black coral by supercritical fluid with carbon dioxide (CO2-SFE).

Mentions: For investigation of the volatile compounds of BCCHs, we used CO2-SFE for extraction and separation. The analysis of essential oils is usually conducted with GC-MS to characterize and identify volatile organic compounds in complex samples [14]. GC-MS combined with retention index (RI) can provide more reliable qualitative analysis of complicated essential oil samples than GC-MS alone because the chromatographic peaks are separated satisfactorily [15]. So compared by mass spectra, the temperature-programmed RIs of these compounds were calculated based on the formula. The RI values were very similar to the RIs reported in the literature, with a margin of a few units (6–7 at most). The total ion chromatogram (TIC) of the volatile constituents from the CO2-SFE is shown in Figure 1. The abundance of each peak was not high in the middle of the TIC because of the low concentration of splitless injections. In total, 15 constituents identified are shown in Table 1, including alkanes, terpenes, esters, fatty acids, and aromatic compounds. Phenol, 2,2′-methylenebis[6-(1,1-dimethylethyl)-4-methyl- was the most abundant compound.


Volatile constituents, inorganic elements and primary screening of bioactivity of black coral cigarette holders.

Bai X, Chen Y, Chen W, Lei H, Shi G - Mar Drugs (2011)

The total ion chromatogram of the volatile constituents from black coral by supercritical fluid with carbon dioxide (CO2-SFE).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111188&req=5

f1-marinedrugs-09-00863: The total ion chromatogram of the volatile constituents from black coral by supercritical fluid with carbon dioxide (CO2-SFE).
Mentions: For investigation of the volatile compounds of BCCHs, we used CO2-SFE for extraction and separation. The analysis of essential oils is usually conducted with GC-MS to characterize and identify volatile organic compounds in complex samples [14]. GC-MS combined with retention index (RI) can provide more reliable qualitative analysis of complicated essential oil samples than GC-MS alone because the chromatographic peaks are separated satisfactorily [15]. So compared by mass spectra, the temperature-programmed RIs of these compounds were calculated based on the formula. The RI values were very similar to the RIs reported in the literature, with a margin of a few units (6–7 at most). The total ion chromatogram (TIC) of the volatile constituents from the CO2-SFE is shown in Figure 1. The abundance of each peak was not high in the middle of the TIC because of the low concentration of splitless injections. In total, 15 constituents identified are shown in Table 1, including alkanes, terpenes, esters, fatty acids, and aromatic compounds. Phenol, 2,2′-methylenebis[6-(1,1-dimethylethyl)-4-methyl- was the most abundant compound.

Bottom Line: In total, 15 components were reliably identified in BC and found to be biologically active.In the bioactivity tests, the BC extract (BCE) showed a scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl radicals by phenanthroline-Fe (II) oxidation and moderate inhibition of Gram-positive microorganisms.The antioxidant and antimicrobial activities of BC, which are related to the active chemical composition, may explain the perceived benefit for cigarette smokers who use BCCHs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.

ABSTRACT
Black corals (BC) have been used for a long time in Chinese medicine, and may have some pharmaceutical functions when used as material for cigarette holders in southeast China. This study is aimed to investigate the bioactivities of volatile constituents in BC and to explore the folklore behind the use of BC cigarette holders (BCCHs). We extracted the volatile constituents of BC by supercritical fluid extraction (SFE) with carbon dioxide (CO₂-SFE), then identified and analyzed the constituents by gas chromatography-mass spectrometry (GC-MS). In total, 15 components were reliably identified in BC and found to be biologically active. These included triethyl phosphate, butylated hydroxytoluene, cedrol, n-hexadecanoic acid, squalene, and cholesterol. Meanwhile 13 inorganic elements (P, Ca, Mg, S, B, Si, Fe, Cu, Zn, Ba, etc.) were determined by inductively coupled plasma spectrometer (ICPS). In the bioactivity tests, the BC extract (BCE) showed a scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl radicals by phenanthroline-Fe (II) oxidation and moderate inhibition of Gram-positive microorganisms. The antioxidant and antimicrobial activities of BC, which are related to the active chemical composition, may explain the perceived benefit for cigarette smokers who use BCCHs.

Show MeSH
Related in: MedlinePlus