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Antiproliferative activity of violaxanthin isolated from bioguided fractionation of Dunaliella tertiolecta extracts.

Pasquet V, Morisset P, Ihammouine S, Chepied A, Aumailley L, Berard JB, Serive B, Kaas R, Lanneluc I, Thiery V, Lafferriere M, Piot JM, Patrice T, Cadoret JP, Picot L - Mar Drugs (2011)

Bottom Line: High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract.Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL⁻¹ (0.17 μM).Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL⁻¹ (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL⁻¹ (66.7 μM).

View Article: PubMed Central - PubMed

Affiliation: UMR CNRS 6250 LIENSs, University of La Rochelle, F-17042 La Rochelle, France.

ABSTRACT
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL⁻¹ (0.17 μM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL⁻¹ (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL⁻¹ (66.7 μM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.

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GI50 (μg·mL−1) of DT DCM extract, F1 fraction and F1.4 sub-fraction on MCF-7.
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f2-marinedrugs-09-00819: GI50 (μg·mL−1) of DT DCM extract, F1 fraction and F1.4 sub-fraction on MCF-7.

Mentions: Fraction 1 (F1) was identified as the only active fraction in the DT DCM extract, with a GI50 = 14.3 μg·mL−1. Decrease of the GI50 value compared to the DCM extract confirmed that this fraction was concentrated in active molecules (Table 2). The GI50 of F2, F3 and F4 were superior to 100 μg·mL−1 (Table 2), indicating that they did not contain potent antiproliferative molecules. F1.2, F1.3 and F1.4 strongly inhibited MCF-7 growth, with GI50 values of 20.5, 18.9 and 11.7 μg·mL−1, respectively (Table 2). The GI50 values of these three sub-fractions were in the range of that of the F1 fraction, and confirmed that the three sub-fractions contained active molecules (Table 2). The GI50 of F1.1 was greater than 40 μg·mL−1. Figure 2 presents the GI50 (μg·mL−1) measured on MCF-7 with the starting DT DCM extract, the F1 fraction and the F1.4 subfraction.


Antiproliferative activity of violaxanthin isolated from bioguided fractionation of Dunaliella tertiolecta extracts.

Pasquet V, Morisset P, Ihammouine S, Chepied A, Aumailley L, Berard JB, Serive B, Kaas R, Lanneluc I, Thiery V, Lafferriere M, Piot JM, Patrice T, Cadoret JP, Picot L - Mar Drugs (2011)

GI50 (μg·mL−1) of DT DCM extract, F1 fraction and F1.4 sub-fraction on MCF-7.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111184&req=5

f2-marinedrugs-09-00819: GI50 (μg·mL−1) of DT DCM extract, F1 fraction and F1.4 sub-fraction on MCF-7.
Mentions: Fraction 1 (F1) was identified as the only active fraction in the DT DCM extract, with a GI50 = 14.3 μg·mL−1. Decrease of the GI50 value compared to the DCM extract confirmed that this fraction was concentrated in active molecules (Table 2). The GI50 of F2, F3 and F4 were superior to 100 μg·mL−1 (Table 2), indicating that they did not contain potent antiproliferative molecules. F1.2, F1.3 and F1.4 strongly inhibited MCF-7 growth, with GI50 values of 20.5, 18.9 and 11.7 μg·mL−1, respectively (Table 2). The GI50 values of these three sub-fractions were in the range of that of the F1 fraction, and confirmed that the three sub-fractions contained active molecules (Table 2). The GI50 of F1.1 was greater than 40 μg·mL−1. Figure 2 presents the GI50 (μg·mL−1) measured on MCF-7 with the starting DT DCM extract, the F1 fraction and the F1.4 subfraction.

Bottom Line: High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract.Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL⁻¹ (0.17 μM).Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL⁻¹ (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL⁻¹ (66.7 μM).

View Article: PubMed Central - PubMed

Affiliation: UMR CNRS 6250 LIENSs, University of La Rochelle, F-17042 La Rochelle, France.

ABSTRACT
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 μg·mL⁻¹ (0.17 μM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 μg·mL⁻¹ (13.3 μM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 μg·mL⁻¹ (66.7 μM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.

Show MeSH
Related in: MedlinePlus