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Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

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Nitrite levels in wounds and plasma of the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 after wounding. Data are expressed as mean ± SD. * P < 0.05 different from same-day between two groups. ** P < 0.01 different from same-day between two groups.
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f6-marinedrugs-09-00696: Nitrite levels in wounds and plasma of the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 after wounding. Data are expressed as mean ± SD. * P < 0.05 different from same-day between two groups. ** P < 0.01 different from same-day between two groups.

Mentions: Nitrite dosage was used as an index of NO synthesis, because nitrite is a stable molecule and accounts for more than 90% of total measurable nitrite and nitrate. Fourteen days after wounding, the nitrite levels were respectively 5-fold and 1.7-fold greater in the skin gelatin-treated group than in the control group in both the wound and plasma (Figure 6).


Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Nitrite levels in wounds and plasma of the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 after wounding. Data are expressed as mean ± SD. * P < 0.05 different from same-day between two groups. ** P < 0.01 different from same-day between two groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111176&req=5

f6-marinedrugs-09-00696: Nitrite levels in wounds and plasma of the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 after wounding. Data are expressed as mean ± SD. * P < 0.05 different from same-day between two groups. ** P < 0.01 different from same-day between two groups.
Mentions: Nitrite dosage was used as an index of NO synthesis, because nitrite is a stable molecule and accounts for more than 90% of total measurable nitrite and nitrate. Fourteen days after wounding, the nitrite levels were respectively 5-fold and 1.7-fold greater in the skin gelatin-treated group than in the control group in both the wound and plasma (Figure 6).

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

Show MeSH
Related in: MedlinePlus