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Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

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Expression of VEGF in wound tissue at day 14 after wounding in (a) vehicle-treated non-diabetic rats; (b) vehicle-treated diabetic rats and (c) skin gelatin-treated diabetic rats. Statistical analysis showed that the extent immunoreactivity of VEGF in the wound areas skin gelatin-treated diabetic rats is greater than that of the vehicle-treated diabetic group. * P < 0.05—significance of the difference between the mean VEGF in the wound area of skin gelatin-treated diabetic rats and that of vehicle-treated diabetic rats (magnification 200×; V, vessel).
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f4-marinedrugs-09-00696: Expression of VEGF in wound tissue at day 14 after wounding in (a) vehicle-treated non-diabetic rats; (b) vehicle-treated diabetic rats and (c) skin gelatin-treated diabetic rats. Statistical analysis showed that the extent immunoreactivity of VEGF in the wound areas skin gelatin-treated diabetic rats is greater than that of the vehicle-treated diabetic group. * P < 0.05—significance of the difference between the mean VEGF in the wound area of skin gelatin-treated diabetic rats and that of vehicle-treated diabetic rats (magnification 200×; V, vessel).

Mentions: The VEGF immunoreactivity of wound sections was examined in the skin gelatin- and vehicle-treated groups at 14 days post-wounding and the numbers of fibroblasts expressing VEGF were counted. As shown in Figure 4, VEGF immunolabeling was striking in the skin gelatin-treated diabetic rats, whereas it was faint in the vehicle-treated diabetic group. The numbers of migrated fibroblasts expressing VEGF in the skin gelatin-treated diabetic group were significantly higher than in the vehicle-treated diabetic group at 14 days post-wounding.


Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Expression of VEGF in wound tissue at day 14 after wounding in (a) vehicle-treated non-diabetic rats; (b) vehicle-treated diabetic rats and (c) skin gelatin-treated diabetic rats. Statistical analysis showed that the extent immunoreactivity of VEGF in the wound areas skin gelatin-treated diabetic rats is greater than that of the vehicle-treated diabetic group. * P < 0.05—significance of the difference between the mean VEGF in the wound area of skin gelatin-treated diabetic rats and that of vehicle-treated diabetic rats (magnification 200×; V, vessel).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111176&req=5

f4-marinedrugs-09-00696: Expression of VEGF in wound tissue at day 14 after wounding in (a) vehicle-treated non-diabetic rats; (b) vehicle-treated diabetic rats and (c) skin gelatin-treated diabetic rats. Statistical analysis showed that the extent immunoreactivity of VEGF in the wound areas skin gelatin-treated diabetic rats is greater than that of the vehicle-treated diabetic group. * P < 0.05—significance of the difference between the mean VEGF in the wound area of skin gelatin-treated diabetic rats and that of vehicle-treated diabetic rats (magnification 200×; V, vessel).
Mentions: The VEGF immunoreactivity of wound sections was examined in the skin gelatin- and vehicle-treated groups at 14 days post-wounding and the numbers of fibroblasts expressing VEGF were counted. As shown in Figure 4, VEGF immunolabeling was striking in the skin gelatin-treated diabetic rats, whereas it was faint in the vehicle-treated diabetic group. The numbers of migrated fibroblasts expressing VEGF in the skin gelatin-treated diabetic group were significantly higher than in the vehicle-treated diabetic group at 14 days post-wounding.

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

Show MeSH
Related in: MedlinePlus