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Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

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Collagen accumulation in wound areas of vehicle- and skin gelatin-treated non-diabetic or diabetic rats. (A) Representative high power view light micrographs (Masson’s trichirome staining) at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Hydroxyproline levels in wound areas of the all treatment groups at day 7 and 14. Data are expressed as mean ± SD. * <0.05 different from same-day between two groups. ** <0.01 different from same-day between two groups.
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f3-marinedrugs-09-00696: Collagen accumulation in wound areas of vehicle- and skin gelatin-treated non-diabetic or diabetic rats. (A) Representative high power view light micrographs (Masson’s trichirome staining) at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Hydroxyproline levels in wound areas of the all treatment groups at day 7 and 14. Data are expressed as mean ± SD. * <0.05 different from same-day between two groups. ** <0.01 different from same-day between two groups.

Mentions: Collagen deposition is an important event in the development of granulation tissue. Compared with the vehicle-treated diabetic group, the wound revealed a marked and robust increase in the organization of collagen fibers bridging the gaps in the skin gelatin-treated diabetic rats. Thus, collagen fibers were more organized and dense in the skin gelatin-treated diabetic group than in the vehicle-treated group (Figure 3A). To confirm these histological observations, hydroxyproline (HP) contents were measured in the lesions by 7 and 14 days after wounding. The results showed that hydroxyproline contents in the skin gelatin-treated diabetic group were higher than in the vehicle-treated diabetic group and significant differences were observed by 7 and 14 days post-wounding (P < 0.01) (Figure 3B).


Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Collagen accumulation in wound areas of vehicle- and skin gelatin-treated non-diabetic or diabetic rats. (A) Representative high power view light micrographs (Masson’s trichirome staining) at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Hydroxyproline levels in wound areas of the all treatment groups at day 7 and 14. Data are expressed as mean ± SD. * <0.05 different from same-day between two groups. ** <0.01 different from same-day between two groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111176&req=5

f3-marinedrugs-09-00696: Collagen accumulation in wound areas of vehicle- and skin gelatin-treated non-diabetic or diabetic rats. (A) Representative high power view light micrographs (Masson’s trichirome staining) at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Hydroxyproline levels in wound areas of the all treatment groups at day 7 and 14. Data are expressed as mean ± SD. * <0.05 different from same-day between two groups. ** <0.01 different from same-day between two groups.
Mentions: Collagen deposition is an important event in the development of granulation tissue. Compared with the vehicle-treated diabetic group, the wound revealed a marked and robust increase in the organization of collagen fibers bridging the gaps in the skin gelatin-treated diabetic rats. Thus, collagen fibers were more organized and dense in the skin gelatin-treated diabetic group than in the vehicle-treated group (Figure 3A). To confirm these histological observations, hydroxyproline (HP) contents were measured in the lesions by 7 and 14 days after wounding. The results showed that hydroxyproline contents in the skin gelatin-treated diabetic group were higher than in the vehicle-treated diabetic group and significant differences were observed by 7 and 14 days post-wounding (P < 0.01) (Figure 3B).

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

Show MeSH
Related in: MedlinePlus