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Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

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Effect of oral skin gelatin administration at the time of wounding on cutaneous wound closure rate in diabetic rats. (A) Representative photos of wounds in the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Group data of wound closure rate expressed as a percentage (%) of the initial wound size. The surface areas of the healing wounds were measured every 2 days and compared by one-way analysis of variance. * P < 0.05, ** P < 0.01—significance of the difference between the mean wound surface area of the vehicle-treated diabetic group and that of the vehicle-treated non-diabetic group. #P < 0.05, ##P < 0.01—significance of the difference between the mean wound surface area of the skin gelatin-treated diabetic group and that of vehicle-treated diabetic group.
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f1-marinedrugs-09-00696: Effect of oral skin gelatin administration at the time of wounding on cutaneous wound closure rate in diabetic rats. (A) Representative photos of wounds in the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Group data of wound closure rate expressed as a percentage (%) of the initial wound size. The surface areas of the healing wounds were measured every 2 days and compared by one-way analysis of variance. * P < 0.05, ** P < 0.01—significance of the difference between the mean wound surface area of the vehicle-treated diabetic group and that of the vehicle-treated non-diabetic group. #P < 0.05, ##P < 0.01—significance of the difference between the mean wound surface area of the skin gelatin-treated diabetic group and that of vehicle-treated diabetic group.

Mentions: Firstly, the difference in wound healing between non-diabetic rats and diabetic rats was measured. As illustrated in Figure 1, the wound area of non-diabetic rats contracted almost to 17% of the original size by day 14 post-wounding, whereas diabetic rats showed significantly delayed wound healing: the wound area contracted to 42% of the original size by day 14. Next, the effect of skin gelatin treatment on wound closure in diabetic rats was investigated. The wound area seemed smaller in the skin gelatin-treated diabetic rats than in the vehicle-treated diabetic rats by day 7 and 14. In order to confirm this observation, the wound area was measured. The percentage of original wound area in the skin gelatin-treated diabetic rats was 16% smaller on day 7 and 35% smaller on day 14 compared to the vehicle-treated diabetic group.


Oral administration of skin gelatin isolated from Chum salmon (Oncorhynchus keta) enhances wound healing in diabetic rats.

Zhang Z, Zhao M, Wang J, Ding Y, Dai X, Li Y - Mar Drugs (2011)

Effect of oral skin gelatin administration at the time of wounding on cutaneous wound closure rate in diabetic rats. (A) Representative photos of wounds in the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Group data of wound closure rate expressed as a percentage (%) of the initial wound size. The surface areas of the healing wounds were measured every 2 days and compared by one-way analysis of variance. * P < 0.05, ** P < 0.01—significance of the difference between the mean wound surface area of the vehicle-treated diabetic group and that of the vehicle-treated non-diabetic group. #P < 0.05, ##P < 0.01—significance of the difference between the mean wound surface area of the skin gelatin-treated diabetic group and that of vehicle-treated diabetic group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3111176&req=5

f1-marinedrugs-09-00696: Effect of oral skin gelatin administration at the time of wounding on cutaneous wound closure rate in diabetic rats. (A) Representative photos of wounds in the non-diabetic and diabetic rats treated with either vehicle or skin gelatin at day 14 (a, vehicle-treated non-diabetic rats; b, vehicle-treated diabetic rats; c, skin gelatin-treated diabetic rats); (B) Group data of wound closure rate expressed as a percentage (%) of the initial wound size. The surface areas of the healing wounds were measured every 2 days and compared by one-way analysis of variance. * P < 0.05, ** P < 0.01—significance of the difference between the mean wound surface area of the vehicle-treated diabetic group and that of the vehicle-treated non-diabetic group. #P < 0.05, ##P < 0.01—significance of the difference between the mean wound surface area of the skin gelatin-treated diabetic group and that of vehicle-treated diabetic group.
Mentions: Firstly, the difference in wound healing between non-diabetic rats and diabetic rats was measured. As illustrated in Figure 1, the wound area of non-diabetic rats contracted almost to 17% of the original size by day 14 post-wounding, whereas diabetic rats showed significantly delayed wound healing: the wound area contracted to 42% of the original size by day 14. Next, the effect of skin gelatin treatment on wound closure in diabetic rats was investigated. The wound area seemed smaller in the skin gelatin-treated diabetic rats than in the vehicle-treated diabetic rats by day 7 and 14. In order to confirm this observation, the wound area was measured. The percentage of original wound area in the skin gelatin-treated diabetic rats was 16% smaller on day 7 and 35% smaller on day 14 compared to the vehicle-treated diabetic group.

Bottom Line: Care for diabetic wounds remains a significant clinical problem.Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response.All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.

ABSTRACT
Care for diabetic wounds remains a significant clinical problem. The present study was aimed at investigating the effect of skin gelatin from Chum Salmon on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 48 rats, of which 32 were diabetes. The diabetic rats were orally treated daily for 14 days with skin gelatin from Chum Salmon (2 g/kg) or its vehicle. Sixteen non-diabetic control rats received the same amount of water as vehicle-treated non-diabetic rats. Rats were killed to assess the rate of wound closure, microvessel density (MVD), vascular endothelial growth factor (VEGF), hydroxyproline (HP) contents in wound tissues and nitrate in plasma and wound tissue at 7 and 14 days after wounding. Skin gelatin-treated diabetic rats showed a better wound closure, increased MVD, VEGF, hydroxyproline and NO contents and a reduced extent of inflammatory response. All parameters were significant (P < 0.05) in comparison to vehicle-treated diabetic group. In light of our finding that skin gelatin of Chum Salmon promotes skin wound repair in diabetic rats, we propose that oral administration of Chum Salmon skin gelatin might be a beneficial method for treating wound disorders associated with diabetes.

Show MeSH
Related in: MedlinePlus