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The association of DNA-dependent protein kinase activity of peripheral blood lymphocytes with prognosis of cancer.

Someya M, Sakata KI, Matsumoto Y, Kamdar RP, Kai M, Toyota M, Hareyama M - Br. J. Cancer (2011)

Bottom Line: DNA-PK activity of PBLs in advanced cancer patients was significantly lower than that in early stage.The DNA-PK activity of PBLs in most patients decreased in response to radiation as the equivalent whole-body dose increased.Therefore, DNA-PK activity in PBLs could be used as a marker to predict the chromosomal instability and poorer prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Sapporo Medical University, School of Medicine, Hokkaido, Japan.

ABSTRACT

Background: Repair of various types of DNA damages is critical for genomic stability. DNA-dependent protein kinase (DNA-PK) has an important role in DNA double-strand break repair. We examined whether there may be a correlation between DNA-PK activity in peripheral blood lymphocytes (PBLs) and survival percentages in various cancer patients. We also investigated the changes of DNA-PK activity in PBLs after radiotherapy.

Methods: A total of 167 of untreated cancer patients participated in this study. Peripheral blood was collected, separated, and centrifuged. DNA-PK activity was measured by DNA-pull-down assay. Chromosomal aberrations were examined by cytogenetic methods.

Results: DNA-PK activity of PBLs in advanced cancer patients was significantly lower than that in early stage. The patients with lower DNA-PK activity in PBLs tended to have the lower disease-specific survivals and distant metastasis-free survivals than those with higher DNA-PK activity in advanced stages. There was also a tendency of inverse correlation between DNA-PK activity and excess fragments. The DNA-PK activity of PBLs in most patients decreased in response to radiation as the equivalent whole-body dose increased.

Conclusion: Cancer patients in advanced stage, with lower DNA-PK activity of PBLs might have higher distant metastasis and exhibit poorer prognosis. Therefore, DNA-PK activity in PBLs could be used as a marker to predict the chromosomal instability and poorer prognosis.

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Related in: MedlinePlus

(A) Changes of DNA-PK activity of PBLs after radiation according to the equivalent whole-body dose. Each line indicated each patient. Each point on each line indicated the sample that was obtained at a given equivalent whole-body dose. (B) Alterations of DNA-PK activity and protein levels of DNA-PK genes (DNA-PKcs, Ku70, and Ku86) in PBLs of two patients (O and T in A) treated with radiotherapy. (C) Recovery of DNA-PK activity of PBLs after radiotherapy. Zero month in time means the DNA-PK activity before radiotherapy. The shaded zone in time indicates periods of radiotherapy.
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fig2: (A) Changes of DNA-PK activity of PBLs after radiation according to the equivalent whole-body dose. Each line indicated each patient. Each point on each line indicated the sample that was obtained at a given equivalent whole-body dose. (B) Alterations of DNA-PK activity and protein levels of DNA-PK genes (DNA-PKcs, Ku70, and Ku86) in PBLs of two patients (O and T in A) treated with radiotherapy. (C) Recovery of DNA-PK activity of PBLs after radiotherapy. Zero month in time means the DNA-PK activity before radiotherapy. The shaded zone in time indicates periods of radiotherapy.

Mentions: Figure 2A demonstrated the alterations of DNA-PK activity of PBLs as a result of radiation in respect to the equivalent whole-body dose.


The association of DNA-dependent protein kinase activity of peripheral blood lymphocytes with prognosis of cancer.

Someya M, Sakata KI, Matsumoto Y, Kamdar RP, Kai M, Toyota M, Hareyama M - Br. J. Cancer (2011)

(A) Changes of DNA-PK activity of PBLs after radiation according to the equivalent whole-body dose. Each line indicated each patient. Each point on each line indicated the sample that was obtained at a given equivalent whole-body dose. (B) Alterations of DNA-PK activity and protein levels of DNA-PK genes (DNA-PKcs, Ku70, and Ku86) in PBLs of two patients (O and T in A) treated with radiotherapy. (C) Recovery of DNA-PK activity of PBLs after radiotherapy. Zero month in time means the DNA-PK activity before radiotherapy. The shaded zone in time indicates periods of radiotherapy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3111168&req=5

fig2: (A) Changes of DNA-PK activity of PBLs after radiation according to the equivalent whole-body dose. Each line indicated each patient. Each point on each line indicated the sample that was obtained at a given equivalent whole-body dose. (B) Alterations of DNA-PK activity and protein levels of DNA-PK genes (DNA-PKcs, Ku70, and Ku86) in PBLs of two patients (O and T in A) treated with radiotherapy. (C) Recovery of DNA-PK activity of PBLs after radiotherapy. Zero month in time means the DNA-PK activity before radiotherapy. The shaded zone in time indicates periods of radiotherapy.
Mentions: Figure 2A demonstrated the alterations of DNA-PK activity of PBLs as a result of radiation in respect to the equivalent whole-body dose.

Bottom Line: DNA-PK activity of PBLs in advanced cancer patients was significantly lower than that in early stage.The DNA-PK activity of PBLs in most patients decreased in response to radiation as the equivalent whole-body dose increased.Therefore, DNA-PK activity in PBLs could be used as a marker to predict the chromosomal instability and poorer prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Sapporo Medical University, School of Medicine, Hokkaido, Japan.

ABSTRACT

Background: Repair of various types of DNA damages is critical for genomic stability. DNA-dependent protein kinase (DNA-PK) has an important role in DNA double-strand break repair. We examined whether there may be a correlation between DNA-PK activity in peripheral blood lymphocytes (PBLs) and survival percentages in various cancer patients. We also investigated the changes of DNA-PK activity in PBLs after radiotherapy.

Methods: A total of 167 of untreated cancer patients participated in this study. Peripheral blood was collected, separated, and centrifuged. DNA-PK activity was measured by DNA-pull-down assay. Chromosomal aberrations were examined by cytogenetic methods.

Results: DNA-PK activity of PBLs in advanced cancer patients was significantly lower than that in early stage. The patients with lower DNA-PK activity in PBLs tended to have the lower disease-specific survivals and distant metastasis-free survivals than those with higher DNA-PK activity in advanced stages. There was also a tendency of inverse correlation between DNA-PK activity and excess fragments. The DNA-PK activity of PBLs in most patients decreased in response to radiation as the equivalent whole-body dose increased.

Conclusion: Cancer patients in advanced stage, with lower DNA-PK activity of PBLs might have higher distant metastasis and exhibit poorer prognosis. Therefore, DNA-PK activity in PBLs could be used as a marker to predict the chromosomal instability and poorer prognosis.

Show MeSH
Related in: MedlinePlus