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Eriocaulon buergerianum extract protects PC12 cells and neurons in zebrafish against 6-hydroxydopamine-induced damage.

Wang M, Zhang Z, Cheang LC, Lin Z, Lee SM - Chin Med (2011)

Bottom Line: EBE inhibited the 6-OHDA-induced decrease in total distance of movement in zebrafish.EBE exhibited significant neuroprotective activities in zebrafish, including recovery of dopaminergic neuron loss caused by 6-OHDA in a dose-dependent manner in vivo, inhibition of 6-OHDA-induced decrease of total distance in movement in zebrafish.The iNOS-NO pathway may be involved.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Av, Padre Tomás Pereira, Taipa, Macao, China. SimonLee@umac.mo.

ABSTRACT

Background: Ericaulon buergerianum (Gujingcao) is an ophthalmic, anti-inflammatory and antimicrobial Chinese medicinal herb. This study aims to investigate the neuroprotective effects of Ericaulon buergerianum ethanol extract (EBE) and to elucidate its underlying action mechanism.

Methods: The viability of dopaminergic (DA) neuron in zebrafish was examined by anti-tyrosine hydroxylase (TH) immunostaining. The locomotor activity of zebrafish was assessed with a digital video tracking system. The viability and cellular damage of the PC12 cells were determined by MTT and LDH assays respectively. The nuclear morphological changes in apoptotic cells were evaluated with DNA staining by Hoechst 33342 dye. Intracellular nitric oxide (NO) was quantified by DAF-FM diacetate staining. The expression of inducible nitric oxide synthase (iNOS) was determined by Western blot.

Results: EBE inhibited the 6-OHDA-induced decrease in total distance of movement in zebrafish. Pretreatments of EBE (25, 50, 100 and 200 μg/ml) increased the viability of 6-OHDA-damaged PC12 cells in a dose dependent manner. Protection against 6-OHDA-induced nuclear fragmentation and accumulation of apoptotic bodies was also observed in EBE pretreated cells. Anti-oxidative (inhibition of NO production and iNOS expression in PC12 cells in vitro) activities of EBE are related to its neuroprotective effects in 6-OHDA-induced DA neuron damage.

Conclusion: EBE exhibited significant neuroprotective activities in zebrafish, including recovery of dopaminergic neuron loss caused by 6-OHDA in a dose-dependent manner in vivo, inhibition of 6-OHDA-induced decrease of total distance in movement in zebrafish. The iNOS-NO pathway may be involved.

No MeSH data available.


Related in: MedlinePlus

EBE inhibited 6-OHDA-induced decrease of total distance of movement in zebrafish. Quantification of the swimming parameter of zebrafish: 4 dpf wild type zebrafish larvae were treated with 6-OHDA (250 μM) and EBE (25 and 50 μg/mL) for three days. The locomotor activity of zebrafish was assessed at seven dpf. All results were expressed as total distance of movement traveled by the larvae, + P = 0.032 versus control group (without 6-OHDA treatment); * P = 0.041, ** P = 0.007 versus 6-OHDA-treated group. All experiments were repeated 3 times.
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Figure 4: EBE inhibited 6-OHDA-induced decrease of total distance of movement in zebrafish. Quantification of the swimming parameter of zebrafish: 4 dpf wild type zebrafish larvae were treated with 6-OHDA (250 μM) and EBE (25 and 50 μg/mL) for three days. The locomotor activity of zebrafish was assessed at seven dpf. All results were expressed as total distance of movement traveled by the larvae, + P = 0.032 versus control group (without 6-OHDA treatment); * P = 0.041, ** P = 0.007 versus 6-OHDA-treated group. All experiments were repeated 3 times.

Mentions: The total distance of movement by 6-OHDA-lesioned fish was significantly decreased when compared with the control group. EBE inhibited 6-OHDA-induced reduction of total distance of movement in zebrafish in a dose dependent manner (Figure 4).


Eriocaulon buergerianum extract protects PC12 cells and neurons in zebrafish against 6-hydroxydopamine-induced damage.

Wang M, Zhang Z, Cheang LC, Lin Z, Lee SM - Chin Med (2011)

EBE inhibited 6-OHDA-induced decrease of total distance of movement in zebrafish. Quantification of the swimming parameter of zebrafish: 4 dpf wild type zebrafish larvae were treated with 6-OHDA (250 μM) and EBE (25 and 50 μg/mL) for three days. The locomotor activity of zebrafish was assessed at seven dpf. All results were expressed as total distance of movement traveled by the larvae, + P = 0.032 versus control group (without 6-OHDA treatment); * P = 0.041, ** P = 0.007 versus 6-OHDA-treated group. All experiments were repeated 3 times.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108929&req=5

Figure 4: EBE inhibited 6-OHDA-induced decrease of total distance of movement in zebrafish. Quantification of the swimming parameter of zebrafish: 4 dpf wild type zebrafish larvae were treated with 6-OHDA (250 μM) and EBE (25 and 50 μg/mL) for three days. The locomotor activity of zebrafish was assessed at seven dpf. All results were expressed as total distance of movement traveled by the larvae, + P = 0.032 versus control group (without 6-OHDA treatment); * P = 0.041, ** P = 0.007 versus 6-OHDA-treated group. All experiments were repeated 3 times.
Mentions: The total distance of movement by 6-OHDA-lesioned fish was significantly decreased when compared with the control group. EBE inhibited 6-OHDA-induced reduction of total distance of movement in zebrafish in a dose dependent manner (Figure 4).

Bottom Line: EBE inhibited the 6-OHDA-induced decrease in total distance of movement in zebrafish.EBE exhibited significant neuroprotective activities in zebrafish, including recovery of dopaminergic neuron loss caused by 6-OHDA in a dose-dependent manner in vivo, inhibition of 6-OHDA-induced decrease of total distance in movement in zebrafish.The iNOS-NO pathway may be involved.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Av, Padre Tomás Pereira, Taipa, Macao, China. SimonLee@umac.mo.

ABSTRACT

Background: Ericaulon buergerianum (Gujingcao) is an ophthalmic, anti-inflammatory and antimicrobial Chinese medicinal herb. This study aims to investigate the neuroprotective effects of Ericaulon buergerianum ethanol extract (EBE) and to elucidate its underlying action mechanism.

Methods: The viability of dopaminergic (DA) neuron in zebrafish was examined by anti-tyrosine hydroxylase (TH) immunostaining. The locomotor activity of zebrafish was assessed with a digital video tracking system. The viability and cellular damage of the PC12 cells were determined by MTT and LDH assays respectively. The nuclear morphological changes in apoptotic cells were evaluated with DNA staining by Hoechst 33342 dye. Intracellular nitric oxide (NO) was quantified by DAF-FM diacetate staining. The expression of inducible nitric oxide synthase (iNOS) was determined by Western blot.

Results: EBE inhibited the 6-OHDA-induced decrease in total distance of movement in zebrafish. Pretreatments of EBE (25, 50, 100 and 200 μg/ml) increased the viability of 6-OHDA-damaged PC12 cells in a dose dependent manner. Protection against 6-OHDA-induced nuclear fragmentation and accumulation of apoptotic bodies was also observed in EBE pretreated cells. Anti-oxidative (inhibition of NO production and iNOS expression in PC12 cells in vitro) activities of EBE are related to its neuroprotective effects in 6-OHDA-induced DA neuron damage.

Conclusion: EBE exhibited significant neuroprotective activities in zebrafish, including recovery of dopaminergic neuron loss caused by 6-OHDA in a dose-dependent manner in vivo, inhibition of 6-OHDA-induced decrease of total distance in movement in zebrafish. The iNOS-NO pathway may be involved.

No MeSH data available.


Related in: MedlinePlus