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Human serum-derived hydroxy long-chain fatty acids exhibit anti-inflammatory and anti-proliferative activity.

Ritchie SA, Jayasinghe D, Davies GF, Ahiahonu P, Ma H, Goodenowe DB - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: Enriched fractions resulted in poly-ADP ribose polymerase (PARP) cleavage, suppression of NFκB, induction of IκBα, and reduction in NOS2 mRNA transcript levels.Our results show that human serum extracts enriched with endogenous long-chain hydroxy fatty acids possess anti-inflammatory and anti-proliferative activity.These findings support a hypothesis that the reduction of these metabolites with age may result in a compromised ability to defend against uncontrolled cell growth and inflammation, and could therefore represent a significant risk for the development of CRC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Phenomenome Discoveries, Inc, Saskatoon, Saskatchewan, Canada. s.ritchie@phenomenome.com

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Western analysis of NFκB, IκBα and NOS2 in SW620 cells treated with three concentrations of GTA+ve and GTA-ve extracts and doxorubicin (DOX). Representative Western blots showing protein levels of NFκB, IκBα and NOS2 in SW620 cells treated with GTA+ve and GTA-ve extracts (see methods).
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Figure 5: Western analysis of NFκB, IκBα and NOS2 in SW620 cells treated with three concentrations of GTA+ve and GTA-ve extracts and doxorubicin (DOX). Representative Western blots showing protein levels of NFκB, IκBα and NOS2 in SW620 cells treated with GTA+ve and GTA-ve extracts (see methods).

Mentions: The structural resemblance of GTAs to the inflammation-resolving protectins and resolvins prompted us to investigate the effect of GTA+ve extract on pro-inflammatory markers. Treatment of SW620 cells for 24 hours resulted in a profound inhibition of NFκB protein level with as little as 20 ug/ml GTA+ve extract, accompanied by an equally profound induction of IκBα, neither of which was observed with GTA-ve extract (Figure 5). Protein levels of nitric oxide synthase (NOS2) were also inhibited in cells treated with the GTA+ve fraction (particularly 20 and 40 ug/ml), but not in cells treated with the GTA-ve fraction (Figure 5).


Human serum-derived hydroxy long-chain fatty acids exhibit anti-inflammatory and anti-proliferative activity.

Ritchie SA, Jayasinghe D, Davies GF, Ahiahonu P, Ma H, Goodenowe DB - J. Exp. Clin. Cancer Res. (2011)

Western analysis of NFκB, IκBα and NOS2 in SW620 cells treated with three concentrations of GTA+ve and GTA-ve extracts and doxorubicin (DOX). Representative Western blots showing protein levels of NFκB, IκBα and NOS2 in SW620 cells treated with GTA+ve and GTA-ve extracts (see methods).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108922&req=5

Figure 5: Western analysis of NFκB, IκBα and NOS2 in SW620 cells treated with three concentrations of GTA+ve and GTA-ve extracts and doxorubicin (DOX). Representative Western blots showing protein levels of NFκB, IκBα and NOS2 in SW620 cells treated with GTA+ve and GTA-ve extracts (see methods).
Mentions: The structural resemblance of GTAs to the inflammation-resolving protectins and resolvins prompted us to investigate the effect of GTA+ve extract on pro-inflammatory markers. Treatment of SW620 cells for 24 hours resulted in a profound inhibition of NFκB protein level with as little as 20 ug/ml GTA+ve extract, accompanied by an equally profound induction of IκBα, neither of which was observed with GTA-ve extract (Figure 5). Protein levels of nitric oxide synthase (NOS2) were also inhibited in cells treated with the GTA+ve fraction (particularly 20 and 40 ug/ml), but not in cells treated with the GTA-ve fraction (Figure 5).

Bottom Line: Enriched fractions resulted in poly-ADP ribose polymerase (PARP) cleavage, suppression of NFκB, induction of IκBα, and reduction in NOS2 mRNA transcript levels.Our results show that human serum extracts enriched with endogenous long-chain hydroxy fatty acids possess anti-inflammatory and anti-proliferative activity.These findings support a hypothesis that the reduction of these metabolites with age may result in a compromised ability to defend against uncontrolled cell growth and inflammation, and could therefore represent a significant risk for the development of CRC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Phenomenome Discoveries, Inc, Saskatoon, Saskatchewan, Canada. s.ritchie@phenomenome.com

Show MeSH
Related in: MedlinePlus