Human serum-derived hydroxy long-chain fatty acids exhibit anti-inflammatory and anti-proliferative activity.
Bottom Line: Enriched fractions resulted in poly-ADP ribose polymerase (PARP) cleavage, suppression of NFκB, induction of IκBα, and reduction in NOS2 mRNA transcript levels.Our results show that human serum extracts enriched with endogenous long-chain hydroxy fatty acids possess anti-inflammatory and anti-proliferative activity.These findings support a hypothesis that the reduction of these metabolites with age may result in a compromised ability to defend against uncontrolled cell growth and inflammation, and could therefore represent a significant risk for the development of CRC.
Affiliation: Phenomenome Discoveries, Inc, Saskatoon, Saskatchewan, Canada. email@example.comShow MeSH
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Mentions: The structural resemblance of GTAs to the inflammation-resolving protectins and resolvins prompted us to investigate the effect of GTA+ve extract on pro-inflammatory markers. Treatment of SW620 cells for 24 hours resulted in a profound inhibition of NFκB protein level with as little as 20 ug/ml GTA+ve extract, accompanied by an equally profound induction of IκBα, neither of which was observed with GTA-ve extract (Figure 5). Protein levels of nitric oxide synthase (NOS2) were also inhibited in cells treated with the GTA+ve fraction (particularly 20 and 40 ug/ml), but not in cells treated with the GTA-ve fraction (Figure 5).
Affiliation: Phenomenome Discoveries, Inc, Saskatoon, Saskatchewan, Canada. firstname.lastname@example.org