Limits...
CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway.

Zheng J, Fu R, Li J, Wang X - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro.Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells in vitro.Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Laboratory, the Second Affiliated hospital of Nanchang University, Nanchang 330006, China.

Show MeSH
CpG-ODN treatment suppressed the caspase-3 activation in Jurkat cells. HepG2 and Jurkat cells were cultured in medium alone or treated with 1 μM CpG-ODN, respectively for 24 h. The unmanipulated HepG2 and Jurkat cells or the CpG-ODN-treated HepG2 and Jurkat cells were co-cultured for 24, respectively. The Jurkat cells were harvested and the contents of activated caspase-3 were determined by flow cytometry analysis. (A) The unmanipulated Jurkat cells; (B) The CpG-ODN-treated Jurkat cells. Data shown are representative histograms from each group of cells from four separate experiments. The percentage of positive cells was indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3108921&req=5

Figure 4: CpG-ODN treatment suppressed the caspase-3 activation in Jurkat cells. HepG2 and Jurkat cells were cultured in medium alone or treated with 1 μM CpG-ODN, respectively for 24 h. The unmanipulated HepG2 and Jurkat cells or the CpG-ODN-treated HepG2 and Jurkat cells were co-cultured for 24, respectively. The Jurkat cells were harvested and the contents of activated caspase-3 were determined by flow cytometry analysis. (A) The unmanipulated Jurkat cells; (B) The CpG-ODN-treated Jurkat cells. Data shown are representative histograms from each group of cells from four separate experiments. The percentage of positive cells was indicated.

Mentions: The activation of caspase-3 is crucial for the intrinsic and extrinsic apoptotic pathways. Accordingly, we selectively examined the activity of caspase-3, a downstream factor of the Fas-FasL pathway. As shown in Figure 4, the levels of activated caspase-3 were significantly reduced in the CpG-ODN-treated Jurkat cells (28.20 ± 0.18%), as compared to unmanipulated Jurkat cells (45.15 ± 0.13%). These data suggested that the CpG-ODN reduced HepG2-induced Jurkat cell death through the caspase-3-dependent apoptotic pathway.


CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway.

Zheng J, Fu R, Li J, Wang X - J. Exp. Clin. Cancer Res. (2011)

CpG-ODN treatment suppressed the caspase-3 activation in Jurkat cells. HepG2 and Jurkat cells were cultured in medium alone or treated with 1 μM CpG-ODN, respectively for 24 h. The unmanipulated HepG2 and Jurkat cells or the CpG-ODN-treated HepG2 and Jurkat cells were co-cultured for 24, respectively. The Jurkat cells were harvested and the contents of activated caspase-3 were determined by flow cytometry analysis. (A) The unmanipulated Jurkat cells; (B) The CpG-ODN-treated Jurkat cells. Data shown are representative histograms from each group of cells from four separate experiments. The percentage of positive cells was indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108921&req=5

Figure 4: CpG-ODN treatment suppressed the caspase-3 activation in Jurkat cells. HepG2 and Jurkat cells were cultured in medium alone or treated with 1 μM CpG-ODN, respectively for 24 h. The unmanipulated HepG2 and Jurkat cells or the CpG-ODN-treated HepG2 and Jurkat cells were co-cultured for 24, respectively. The Jurkat cells were harvested and the contents of activated caspase-3 were determined by flow cytometry analysis. (A) The unmanipulated Jurkat cells; (B) The CpG-ODN-treated Jurkat cells. Data shown are representative histograms from each group of cells from four separate experiments. The percentage of positive cells was indicated.
Mentions: The activation of caspase-3 is crucial for the intrinsic and extrinsic apoptotic pathways. Accordingly, we selectively examined the activity of caspase-3, a downstream factor of the Fas-FasL pathway. As shown in Figure 4, the levels of activated caspase-3 were significantly reduced in the CpG-ODN-treated Jurkat cells (28.20 ± 0.18%), as compared to unmanipulated Jurkat cells (45.15 ± 0.13%). These data suggested that the CpG-ODN reduced HepG2-induced Jurkat cell death through the caspase-3-dependent apoptotic pathway.

Bottom Line: To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro.Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells in vitro.Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Laboratory, the Second Affiliated hospital of Nanchang University, Nanchang 330006, China.

Show MeSH