CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway.
Bottom Line: To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro.Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells in vitro.Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.
Affiliation: Department of Clinical Laboratory, the Second Affiliated hospital of Nanchang University, Nanchang 330006, China.Show MeSH
Mentions: The activation of caspase-3 is crucial for the intrinsic and extrinsic apoptotic pathways. Accordingly, we selectively examined the activity of caspase-3, a downstream factor of the Fas-FasL pathway. As shown in Figure 4, the levels of activated caspase-3 were significantly reduced in the CpG-ODN-treated Jurkat cells (28.20 ± 0.18%), as compared to unmanipulated Jurkat cells (45.15 ± 0.13%). These data suggested that the CpG-ODN reduced HepG2-induced Jurkat cell death through the caspase-3-dependent apoptotic pathway.
Affiliation: Department of Clinical Laboratory, the Second Affiliated hospital of Nanchang University, Nanchang 330006, China.