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CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway.

Zheng J, Fu R, Li J, Wang X - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro.Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells in vitro.Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Laboratory, the Second Affiliated hospital of Nanchang University, Nanchang 330006, China.

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Treatment with CpG-ODN inhibited the expression of Fas in Jurkat cells. Jurkat cells were treated with 1 μM CpG-ODN for 24 h, and the cells were collected. The intracellular expression of Fas was examined by qRT-PCR (A) and FCM (B). Data are expressed as mean% ± SEM of each group of the cells from four separate experiments. *p < 0.05 vs. the controls.
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Figure 2: Treatment with CpG-ODN inhibited the expression of Fas in Jurkat cells. Jurkat cells were treated with 1 μM CpG-ODN for 24 h, and the cells were collected. The intracellular expression of Fas was examined by qRT-PCR (A) and FCM (B). Data are expressed as mean% ± SEM of each group of the cells from four separate experiments. *p < 0.05 vs. the controls.

Mentions: Next, we tested whether treatment with CpG-ODN could modulate the expression of Fas in Jurkat cells. Jurkat cells were treated with 1 μM CpG-ODN for 24 h. The cells were harvested and the relative levels of Fas mRNA transcripts to control GAPDH were determined by quantitative RT-PCR (Figure 2A). Clearly, the relative levels of Fas mRNA transcripts in the CpG-ODN-treated Jurkat cells were reduced to 65%, as compared with that of unmanipulated controls. Furthermore, the expression of Fas in Jurkat cells was also examined by flow cytometry analysis. The frequency of Fas-expressing Jurkat cells was significantly reduced from 54% ± 2% to 35% ± 1% (Figure 2B). Therefore, CpG-ODN treatment down-regulated the Fas mRNA transcription and protein expression in Jurkat cells in vitro.


CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway.

Zheng J, Fu R, Li J, Wang X - J. Exp. Clin. Cancer Res. (2011)

Treatment with CpG-ODN inhibited the expression of Fas in Jurkat cells. Jurkat cells were treated with 1 μM CpG-ODN for 24 h, and the cells were collected. The intracellular expression of Fas was examined by qRT-PCR (A) and FCM (B). Data are expressed as mean% ± SEM of each group of the cells from four separate experiments. *p < 0.05 vs. the controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108921&req=5

Figure 2: Treatment with CpG-ODN inhibited the expression of Fas in Jurkat cells. Jurkat cells were treated with 1 μM CpG-ODN for 24 h, and the cells were collected. The intracellular expression of Fas was examined by qRT-PCR (A) and FCM (B). Data are expressed as mean% ± SEM of each group of the cells from four separate experiments. *p < 0.05 vs. the controls.
Mentions: Next, we tested whether treatment with CpG-ODN could modulate the expression of Fas in Jurkat cells. Jurkat cells were treated with 1 μM CpG-ODN for 24 h. The cells were harvested and the relative levels of Fas mRNA transcripts to control GAPDH were determined by quantitative RT-PCR (Figure 2A). Clearly, the relative levels of Fas mRNA transcripts in the CpG-ODN-treated Jurkat cells were reduced to 65%, as compared with that of unmanipulated controls. Furthermore, the expression of Fas in Jurkat cells was also examined by flow cytometry analysis. The frequency of Fas-expressing Jurkat cells was significantly reduced from 54% ± 2% to 35% ± 1% (Figure 2B). Therefore, CpG-ODN treatment down-regulated the Fas mRNA transcription and protein expression in Jurkat cells in vitro.

Bottom Line: To explore the potential role of CpG motif-containing oligonucleotides (CpG-ODN) in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro, and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro.Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells in vitro.Apparently, CpG-ODN treatment may be a potential therapeutic reagent for HCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Laboratory, the Second Affiliated hospital of Nanchang University, Nanchang 330006, China.

Show MeSH
Related in: MedlinePlus