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Differential regulation of diacylglycerol kinase isoform in human failing hearts.

Bilim O, Shishido T, Toyama S, Suzuki S, Sasaki T, Kitahara T, Sadahiro M, Takeishi Y, Kubota I - J Cardiothorac Surg (2011)

Bottom Line: Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure.DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG.We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan.

ABSTRACT
Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure. DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG. Expressions of DGK isoforms α, ε, and ζ in rodent hearts have been detected; however, the expression and alteration of DGK isoforms in a failing human heart has not yet been examined. In this study, we detected mRNA expressions of DGK isoforms γ, η, ε, and ζ in failing human heart samples obtained from patients undergoing cardiovascular surgery with cardiopulmonary bypass. Furthermore, we investigated modulation of DGK isoform expression in these hearts. We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged. This is the first report that describes the differential regulation of DGK isoforms in normal and failing human hearts.

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Diacylglycerol kinase (DGK)η (A) and DGKζ (B) mRNA expressions in right atrial samples obtained from patients with and without heart failure.
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Figure 2: Diacylglycerol kinase (DGK)η (A) and DGKζ (B) mRNA expressions in right atrial samples obtained from patients with and without heart failure.

Mentions: To investigate the changes in mRNA levels of the DGK isoforms in patients with volume-overloaded atria, we examined the expression levels of DGKγ, DGKη, DGKε, and DGKζ isoforms in the right atrium specimens obtained from heart failure patients and compared them with the corresponding expression levels in the control heart samples. Volume overload caused changes in the expression levels of DGKη and DGKζ. Expression level of DGKη was significantly increased (Figure 2A), while that of DGKζ was significantly decreased (Figure 2B). In contrast, expression levels of DGKγ and DGKε remained unchanged in the patients with chronic overload in the right atrium.


Differential regulation of diacylglycerol kinase isoform in human failing hearts.

Bilim O, Shishido T, Toyama S, Suzuki S, Sasaki T, Kitahara T, Sadahiro M, Takeishi Y, Kubota I - J Cardiothorac Surg (2011)

Diacylglycerol kinase (DGK)η (A) and DGKζ (B) mRNA expressions in right atrial samples obtained from patients with and without heart failure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108920&req=5

Figure 2: Diacylglycerol kinase (DGK)η (A) and DGKζ (B) mRNA expressions in right atrial samples obtained from patients with and without heart failure.
Mentions: To investigate the changes in mRNA levels of the DGK isoforms in patients with volume-overloaded atria, we examined the expression levels of DGKγ, DGKη, DGKε, and DGKζ isoforms in the right atrium specimens obtained from heart failure patients and compared them with the corresponding expression levels in the control heart samples. Volume overload caused changes in the expression levels of DGKη and DGKζ. Expression level of DGKη was significantly increased (Figure 2A), while that of DGKζ was significantly decreased (Figure 2B). In contrast, expression levels of DGKγ and DGKε remained unchanged in the patients with chronic overload in the right atrium.

Bottom Line: Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure.DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG.We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan.

ABSTRACT
Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure. DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG. Expressions of DGK isoforms α, ε, and ζ in rodent hearts have been detected; however, the expression and alteration of DGK isoforms in a failing human heart has not yet been examined. In this study, we detected mRNA expressions of DGK isoforms γ, η, ε, and ζ in failing human heart samples obtained from patients undergoing cardiovascular surgery with cardiopulmonary bypass. Furthermore, we investigated modulation of DGK isoform expression in these hearts. We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged. This is the first report that describes the differential regulation of DGK isoforms in normal and failing human hearts.

Show MeSH
Related in: MedlinePlus