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Differential regulation of diacylglycerol kinase isoform in human failing hearts.

Bilim O, Shishido T, Toyama S, Suzuki S, Sasaki T, Kitahara T, Sadahiro M, Takeishi Y, Kubota I - J Cardiothorac Surg (2011)

Bottom Line: Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure.DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG.We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan.

ABSTRACT
Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure. DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG. Expressions of DGK isoforms α, ε, and ζ in rodent hearts have been detected; however, the expression and alteration of DGK isoforms in a failing human heart has not yet been examined. In this study, we detected mRNA expressions of DGK isoforms γ, η, ε, and ζ in failing human heart samples obtained from patients undergoing cardiovascular surgery with cardiopulmonary bypass. Furthermore, we investigated modulation of DGK isoform expression in these hearts. We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged. This is the first report that describes the differential regulation of DGK isoforms in normal and failing human hearts.

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Expressions of diacylglycerol kinase (DGK) isoforms in normal human right atrium.
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Figure 1: Expressions of diacylglycerol kinase (DGK) isoforms in normal human right atrium.

Mentions: The expression of the DGK isoforms in the human right atrium was examined in the control heart specimens by using RT-PCR. RT-PCR analysis performed using oligonucleotide primers specific for the 10 human DGK isoforms revealed 4 DGK isoforms DGKγ, DGKη, DGKε, and DGKζ in normal human hearts (Figure 1).


Differential regulation of diacylglycerol kinase isoform in human failing hearts.

Bilim O, Shishido T, Toyama S, Suzuki S, Sasaki T, Kitahara T, Sadahiro M, Takeishi Y, Kubota I - J Cardiothorac Surg (2011)

Expressions of diacylglycerol kinase (DGK) isoforms in normal human right atrium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108920&req=5

Figure 1: Expressions of diacylglycerol kinase (DGK) isoforms in normal human right atrium.
Mentions: The expression of the DGK isoforms in the human right atrium was examined in the control heart specimens by using RT-PCR. RT-PCR analysis performed using oligonucleotide primers specific for the 10 human DGK isoforms revealed 4 DGK isoforms DGKγ, DGKη, DGKε, and DGKζ in normal human hearts (Figure 1).

Bottom Line: Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure.DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG.We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cardiology, Pulmonology, and Nephrology, Yamagata University School of Medicine, Yamagata, Japan.

ABSTRACT
Evidence from several studies indicates the importance of Gαq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG), and protein kinase C, in the development of heart failure. DAG kinase (DGK) acts as an endogenous regulator of GPCR signaling pathway by catalyzing and regulating DAG. Expressions of DGK isoforms α, ε, and ζ in rodent hearts have been detected; however, the expression and alteration of DGK isoforms in a failing human heart has not yet been examined. In this study, we detected mRNA expressions of DGK isoforms γ, η, ε, and ζ in failing human heart samples obtained from patients undergoing cardiovascular surgery with cardiopulmonary bypass. Furthermore, we investigated modulation of DGK isoform expression in these hearts. We found that expressions of DGKη and DGKζ were increased and decreased, respectively, whereas those of DGKγ and DGKε remained unchanged. This is the first report that describes the differential regulation of DGK isoforms in normal and failing human hearts.

Show MeSH
Related in: MedlinePlus