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Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.

Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y - Mol. Vis. (2011)

Bottom Line: In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts.Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Purpose: To investigate the profile of T-helper type 17 (Th17) cell-related cytokines (interleukin-23 [IL-23], IL-27, IL-17 and interferon-γ [IFN-γ]) in postoperative inflammation in patients with Behcet disease (BD) after cataract surgery.

Methods: Serum was collected from seven BD patients with complicated cataract, and from nine controls with uncomplicated cataract, before cataract surgery, and again 1, 7, 30, and 90 days after surgery. In addition, aqueous humor was collected at commencement of surgery. The protein levels of IL-23, IL-27, IL-17, and IFN-γ in the serum and in the aqueous humor were measured by an enzyme-linked immunosorbent assay. A laser flare-cell photometer was used to quantify intraocular inflammation.

Results: Serum IL-23, IL-27, and IFN-γ levels were significantly increased after cataract surgery in the BD versus the control patients. In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts. Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).

Conclusions: Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

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Related in: MedlinePlus

The correlation between serum levels of Th17 cytokines (pg/ml) and inflammatory variables(flare value: pc/ms; cell counts: cells/0.5mm3) in BD patients.
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f5: The correlation between serum levels of Th17 cytokines (pg/ml) and inflammatory variables(flare value: pc/ms; cell counts: cells/0.5mm3) in BD patients.

Mentions: The results showed that, for both groups, the aqueous flare value and cell count increased after surgery and achieved the highest level on day 1 post-surgery, and then decreased rapidly during the first week, which was then followed by a more gradual reduction. Serum IFN-γ and IL-27 levels correlated strongly with aqueous flare value and cell counts in BD patients, but that serum IL-23 levels did not (Table 2 and Figure 5). Of note, serum IL-27 levels in BD patients were significantly associated with serum IFN-γ levels (r=0.796; p=0.002). No correlation has been found between serum IL-23 levels and postoperative inflammation, although serum IL-23 levels were elevated after surgery in control subjects.


Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.

Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y - Mol. Vis. (2011)

The correlation between serum levels of Th17 cytokines (pg/ml) and inflammatory variables(flare value: pc/ms; cell counts: cells/0.5mm3) in BD patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108896&req=5

f5: The correlation between serum levels of Th17 cytokines (pg/ml) and inflammatory variables(flare value: pc/ms; cell counts: cells/0.5mm3) in BD patients.
Mentions: The results showed that, for both groups, the aqueous flare value and cell count increased after surgery and achieved the highest level on day 1 post-surgery, and then decreased rapidly during the first week, which was then followed by a more gradual reduction. Serum IFN-γ and IL-27 levels correlated strongly with aqueous flare value and cell counts in BD patients, but that serum IL-23 levels did not (Table 2 and Figure 5). Of note, serum IL-27 levels in BD patients were significantly associated with serum IFN-γ levels (r=0.796; p=0.002). No correlation has been found between serum IL-23 levels and postoperative inflammation, although serum IL-23 levels were elevated after surgery in control subjects.

Bottom Line: In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts.Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Purpose: To investigate the profile of T-helper type 17 (Th17) cell-related cytokines (interleukin-23 [IL-23], IL-27, IL-17 and interferon-γ [IFN-γ]) in postoperative inflammation in patients with Behcet disease (BD) after cataract surgery.

Methods: Serum was collected from seven BD patients with complicated cataract, and from nine controls with uncomplicated cataract, before cataract surgery, and again 1, 7, 30, and 90 days after surgery. In addition, aqueous humor was collected at commencement of surgery. The protein levels of IL-23, IL-27, IL-17, and IFN-γ in the serum and in the aqueous humor were measured by an enzyme-linked immunosorbent assay. A laser flare-cell photometer was used to quantify intraocular inflammation.

Results: Serum IL-23, IL-27, and IFN-γ levels were significantly increased after cataract surgery in the BD versus the control patients. In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts. Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).

Conclusions: Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

Show MeSH
Related in: MedlinePlus