Limits...
Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.

Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y - Mol. Vis. (2011)

Bottom Line: In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts.Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Purpose: To investigate the profile of T-helper type 17 (Th17) cell-related cytokines (interleukin-23 [IL-23], IL-27, IL-17 and interferon-γ [IFN-γ]) in postoperative inflammation in patients with Behcet disease (BD) after cataract surgery.

Methods: Serum was collected from seven BD patients with complicated cataract, and from nine controls with uncomplicated cataract, before cataract surgery, and again 1, 7, 30, and 90 days after surgery. In addition, aqueous humor was collected at commencement of surgery. The protein levels of IL-23, IL-27, IL-17, and IFN-γ in the serum and in the aqueous humor were measured by an enzyme-linked immunosorbent assay. A laser flare-cell photometer was used to quantify intraocular inflammation.

Results: Serum IL-23, IL-27, and IFN-γ levels were significantly increased after cataract surgery in the BD versus the control patients. In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts. Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).

Conclusions: Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

Show MeSH

Related in: MedlinePlus

The cytokine levels (pg/ml) of BD patients and controls before surgery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3108896&req=5

f1: The cytokine levels (pg/ml) of BD patients and controls before surgery.

Mentions: Before surgery, IL-17 was undetectable in the serum of all BD patients and control subjects. IL-23 was expressed in serum of both BD patients and control subjects. The low concentrations of IL-27 and IFN-γ were detected in serum of BD patients. However, IL-27 and IFN-γ were lower than the detection level in control subjects (Figure 1). AqH samples were collected at the initiation of cataract surgery, and protein levels of the Th17-associated cytokines were measured. The results showed that IL-23, IL-27, IL-17, and IFN-γ were undetectable in all AqH samples of both BD patients and controls.


Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.

Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y - Mol. Vis. (2011)

The cytokine levels (pg/ml) of BD patients and controls before surgery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108896&req=5

f1: The cytokine levels (pg/ml) of BD patients and controls before surgery.
Mentions: Before surgery, IL-17 was undetectable in the serum of all BD patients and control subjects. IL-23 was expressed in serum of both BD patients and control subjects. The low concentrations of IL-27 and IFN-γ were detected in serum of BD patients. However, IL-27 and IFN-γ were lower than the detection level in control subjects (Figure 1). AqH samples were collected at the initiation of cataract surgery, and protein levels of the Th17-associated cytokines were measured. The results showed that IL-23, IL-27, IL-17, and IFN-γ were undetectable in all AqH samples of both BD patients and controls.

Bottom Line: In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts.Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Purpose: To investigate the profile of T-helper type 17 (Th17) cell-related cytokines (interleukin-23 [IL-23], IL-27, IL-17 and interferon-γ [IFN-γ]) in postoperative inflammation in patients with Behcet disease (BD) after cataract surgery.

Methods: Serum was collected from seven BD patients with complicated cataract, and from nine controls with uncomplicated cataract, before cataract surgery, and again 1, 7, 30, and 90 days after surgery. In addition, aqueous humor was collected at commencement of surgery. The protein levels of IL-23, IL-27, IL-17, and IFN-γ in the serum and in the aqueous humor were measured by an enzyme-linked immunosorbent assay. A laser flare-cell photometer was used to quantify intraocular inflammation.

Results: Serum IL-23, IL-27, and IFN-γ levels were significantly increased after cataract surgery in the BD versus the control patients. In the BD patients, serum levels of IFN-γ and IL-27 correlated strongly with aqueous flare values and cell counts. Remarkably, the levels of serum IL-27 were significantly associated with serum IFN-γ levels in BD patients (r=0.796; p=0.002).

Conclusions: Our data indicates that serum IFN-γ and IL-27 levels are significantly elevated in BD versus control patients and are strongly associated with post-operative intraocular inflammation.

Show MeSH
Related in: MedlinePlus