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Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco.

Ennaji H, Timinouni M, Ennaji MM, Hassar M, Cohen N - Infect Drug Resist (2008)

Bottom Line: Strains of L. monocytogenes were separated by multiplex PCR; two serogroups IIb and IVb were thus differentiated.Antibiotic susceptibility of L. monocytogenes to 21 antibiotics was determined by the disk diffusion method.All isolates were susceptible to a wide range of the tested antibiotics with the exception of nalidixic acid, colistine and cephalosporins second and third generation for which they were all resistant.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire de Microbiologie et Hygiène des Aliments et de l 'Environnement, Institut Pasteur du Maroc., Casablanca, Morocco;

ABSTRACT
This study was carried out on 426 samples of raw meats collected from butcheries and supermarkets in Casablanca, Morocco. The samples were examined for the occurrence of Listeria species. Strains of Listeria monocytogenes were characterized by several biochemical tests and confirmed by polymerase chain reaction (PCR). β-hemolytic cultures and nonhemolytic isolates were tested for biochemical properties with the Listeria API test. Among the 43 Listeria species isolates; we identified 10 strains for L. monocytogenes (23.3%), 31 strains for L. innocua (72.1%) and 2 strains for L. welshimeri (4.6%). Strains of L. monocytogenes were separated by multiplex PCR; two serogroups IIb and IVb were thus differentiated. Antibiotic susceptibility of L. monocytogenes to 21 antibiotics was determined by the disk diffusion method. All isolates were susceptible to a wide range of the tested antibiotics with the exception of nalidixic acid, colistine and cephalosporins second and third generation for which they were all resistant.

No MeSH data available.


Related in: MedlinePlus

Identification by PCR of Listeria monocytogenes.Notes: Lane: 1, molecular size marker φ × 174 diggers by Hae III; Lanes: 2–11, Listeria monocytogenes samples; Lane: 12, reference strain of Listeria monocytogenes (positive control); Lane: 13, negative control.
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f1-idr-1-045: Identification by PCR of Listeria monocytogenes.Notes: Lane: 1, molecular size marker φ × 174 diggers by Hae III; Lanes: 2–11, Listeria monocytogenes samples; Lane: 12, reference strain of Listeria monocytogenes (positive control); Lane: 13, negative control.

Mentions: To confirm the identification of the ten strains characterized by biochemical tests, we have done PCR analyses. We used two primers derived from the virulence associated gene hly specifically those that amplifying the 234 bp region of the listeriolysin O gene. PCR amplification gives all amplicons with same molecular size as shown in Figure 1.


Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco.

Ennaji H, Timinouni M, Ennaji MM, Hassar M, Cohen N - Infect Drug Resist (2008)

Identification by PCR of Listeria monocytogenes.Notes: Lane: 1, molecular size marker φ × 174 diggers by Hae III; Lanes: 2–11, Listeria monocytogenes samples; Lane: 12, reference strain of Listeria monocytogenes (positive control); Lane: 13, negative control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108722&req=5

f1-idr-1-045: Identification by PCR of Listeria monocytogenes.Notes: Lane: 1, molecular size marker φ × 174 diggers by Hae III; Lanes: 2–11, Listeria monocytogenes samples; Lane: 12, reference strain of Listeria monocytogenes (positive control); Lane: 13, negative control.
Mentions: To confirm the identification of the ten strains characterized by biochemical tests, we have done PCR analyses. We used two primers derived from the virulence associated gene hly specifically those that amplifying the 234 bp region of the listeriolysin O gene. PCR amplification gives all amplicons with same molecular size as shown in Figure 1.

Bottom Line: Strains of L. monocytogenes were separated by multiplex PCR; two serogroups IIb and IVb were thus differentiated.Antibiotic susceptibility of L. monocytogenes to 21 antibiotics was determined by the disk diffusion method.All isolates were susceptible to a wide range of the tested antibiotics with the exception of nalidixic acid, colistine and cephalosporins second and third generation for which they were all resistant.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire de Microbiologie et Hygiène des Aliments et de l 'Environnement, Institut Pasteur du Maroc., Casablanca, Morocco;

ABSTRACT
This study was carried out on 426 samples of raw meats collected from butcheries and supermarkets in Casablanca, Morocco. The samples were examined for the occurrence of Listeria species. Strains of Listeria monocytogenes were characterized by several biochemical tests and confirmed by polymerase chain reaction (PCR). β-hemolytic cultures and nonhemolytic isolates were tested for biochemical properties with the Listeria API test. Among the 43 Listeria species isolates; we identified 10 strains for L. monocytogenes (23.3%), 31 strains for L. innocua (72.1%) and 2 strains for L. welshimeri (4.6%). Strains of L. monocytogenes were separated by multiplex PCR; two serogroups IIb and IVb were thus differentiated. Antibiotic susceptibility of L. monocytogenes to 21 antibiotics was determined by the disk diffusion method. All isolates were susceptible to a wide range of the tested antibiotics with the exception of nalidixic acid, colistine and cephalosporins second and third generation for which they were all resistant.

No MeSH data available.


Related in: MedlinePlus