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Baicalin induced dendritic cell apoptosis in vitro.

Zhang H, Jiao Q, Gong Q, Zhang Y, Zhang W, Hu Z - Front Pharmacol (2011)

Bottom Line: This study was aimed to investigate the effects of baicalin (BA), a major flavonoid constituent found in the herb Baikal skullcap, on dendritic cells (DCs).Exposure of DCs to BA (2-50 μM) during BM cell differentiation showed no effects on the up-regulation of CD80/CD86 expression on DCs in response to LPS stimulation, but reduced DCs recovery by inducing apoptosis, and significantly inhibited the release of IL-12 to culture supernatants.These results demonstrate that BA induces selective apoptosis in immature DCs possibly through mitochondria-mediated pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Second Military Medical University Shanghai, China.

ABSTRACT
This study was aimed to investigate the effects of baicalin (BA), a major flavonoid constituent found in the herb Baikal skullcap, on dendritic cells (DCs). DCs were generated by culturing murine bone marrow (BM) cells for 6 days with granulocyte-macrophage colony-stimulating factor and interleukin (IL)-4, and lipopolysaccharide (LPS) was added on day 5 to stimulate DCs maturation. The expression levels of DC maturity markers (CD80/CD86) were assessed by flow cytometry using direct immunofluorescence method. IL-12 levels in the culture supernatants were assayed by ELISA. Apoptosis of DCs was analyzed by flow cytometry after annexin V/propidium iodide staining. The mitochondrial membrane potential (Δψ(m)) changes were measured by using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Exposure of DCs to BA (2-50 μM) during BM cell differentiation showed no effects on the up-regulation of CD80/CD86 expression on DCs in response to LPS stimulation, but reduced DCs recovery by inducing apoptosis, and significantly inhibited the release of IL-12 to culture supernatants. BA-induced DC apoptosis in a time- and dose-dependent way, and immature DCs were more sensitive for BA-induced apoptosis than mature DC. BA also induced Δψ(m) changes in DCs. These results demonstrate that BA induces selective apoptosis in immature DCs possibly through mitochondria-mediated pathway.

No MeSH data available.


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The chemical structure of BA.
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Figure 1: The chemical structure of BA.

Mentions: Baicalin (BA) is a major flavonoid constituent (Figure 1) found in the traditional Chinese medicinal herb Baikal skullcap (Scutellaria baicalensis Georgi), known as “Huang qin” in China and “Ogon” in Japan, which has been widely used for the treatment of various diseases such as pneumonia, hepatitis, and diarrhea (Huang et al., 1986). Previous studies have demonstrated that BA possesses a wide range of pharmacological and biological activities such as anti-inflammatory, anti-allergic, antimicrobial, antioxidant, and anti-tumor properties (Zhang et al., 2003). Its anti-inflammatory activity has been estimated in various animal models of acute and chronic inflammation (Kubo et al., 1984; Lin and Shieh, 1996; Zeng et al., 2007). Evidence shows that its anti-inflammatory actions are associated with the suppression of functions of various inflammatory cells and inhibition the production of pro-inflammatory mediators such as TNF-α, interleukin (IL)-1, and PGE2 (Chung et al., 1995; Krakauer et al., 2001). Recently, it is reported that BA can inhibit the proliferation of mouse T-lymphocytes stimulated with various mitogens and arrest lymphocytes in G0/G1 phase (Li et al., 2009). The results of these studies indicate that BA may have pronounced immunoregulatory properties, although the underlying mechanisms still remain to be fully elucidated.


Baicalin induced dendritic cell apoptosis in vitro.

Zhang H, Jiao Q, Gong Q, Zhang Y, Zhang W, Hu Z - Front Pharmacol (2011)

The chemical structure of BA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108619&req=5

Figure 1: The chemical structure of BA.
Mentions: Baicalin (BA) is a major flavonoid constituent (Figure 1) found in the traditional Chinese medicinal herb Baikal skullcap (Scutellaria baicalensis Georgi), known as “Huang qin” in China and “Ogon” in Japan, which has been widely used for the treatment of various diseases such as pneumonia, hepatitis, and diarrhea (Huang et al., 1986). Previous studies have demonstrated that BA possesses a wide range of pharmacological and biological activities such as anti-inflammatory, anti-allergic, antimicrobial, antioxidant, and anti-tumor properties (Zhang et al., 2003). Its anti-inflammatory activity has been estimated in various animal models of acute and chronic inflammation (Kubo et al., 1984; Lin and Shieh, 1996; Zeng et al., 2007). Evidence shows that its anti-inflammatory actions are associated with the suppression of functions of various inflammatory cells and inhibition the production of pro-inflammatory mediators such as TNF-α, interleukin (IL)-1, and PGE2 (Chung et al., 1995; Krakauer et al., 2001). Recently, it is reported that BA can inhibit the proliferation of mouse T-lymphocytes stimulated with various mitogens and arrest lymphocytes in G0/G1 phase (Li et al., 2009). The results of these studies indicate that BA may have pronounced immunoregulatory properties, although the underlying mechanisms still remain to be fully elucidated.

Bottom Line: This study was aimed to investigate the effects of baicalin (BA), a major flavonoid constituent found in the herb Baikal skullcap, on dendritic cells (DCs).Exposure of DCs to BA (2-50 μM) during BM cell differentiation showed no effects on the up-regulation of CD80/CD86 expression on DCs in response to LPS stimulation, but reduced DCs recovery by inducing apoptosis, and significantly inhibited the release of IL-12 to culture supernatants.These results demonstrate that BA induces selective apoptosis in immature DCs possibly through mitochondria-mediated pathway.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Second Military Medical University Shanghai, China.

ABSTRACT
This study was aimed to investigate the effects of baicalin (BA), a major flavonoid constituent found in the herb Baikal skullcap, on dendritic cells (DCs). DCs were generated by culturing murine bone marrow (BM) cells for 6 days with granulocyte-macrophage colony-stimulating factor and interleukin (IL)-4, and lipopolysaccharide (LPS) was added on day 5 to stimulate DCs maturation. The expression levels of DC maturity markers (CD80/CD86) were assessed by flow cytometry using direct immunofluorescence method. IL-12 levels in the culture supernatants were assayed by ELISA. Apoptosis of DCs was analyzed by flow cytometry after annexin V/propidium iodide staining. The mitochondrial membrane potential (Δψ(m)) changes were measured by using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Exposure of DCs to BA (2-50 μM) during BM cell differentiation showed no effects on the up-regulation of CD80/CD86 expression on DCs in response to LPS stimulation, but reduced DCs recovery by inducing apoptosis, and significantly inhibited the release of IL-12 to culture supernatants. BA-induced DC apoptosis in a time- and dose-dependent way, and immature DCs were more sensitive for BA-induced apoptosis than mature DC. BA also induced Δψ(m) changes in DCs. These results demonstrate that BA induces selective apoptosis in immature DCs possibly through mitochondria-mediated pathway.

No MeSH data available.


Related in: MedlinePlus