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Chemical elemental distribution and soil DNA fingerprints provide the critical evidence in murder case investigation.

Concheri G, Bertoldi D, Polone E, Otto S, Larcher R, Squartini A - PLoS ONE (2011)

Bottom Line: The first soil was collected on the crime scene, along a corn field, while the second was found in trace amounts on the carpet of a car impounded from the main suspect in a distant location.The matching similarity of the two soils was proven by crossing the results of two independent techniques: a) elemental analysis via inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES) approaches, and b) amplified ribosomal DNA restriction analysis by gel electrophoresis (ARDRA).Besides introducing the novel application of these methods to forensic disciplines, the highly accurate level of resolution observed, opens new possibilities also in the fields of soil typing and tracking, historical analyses, geochemical surveys and global land mapping.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biotecnologie Agrarie, Università di Padova, Legnaro (Padova) Italy.

ABSTRACT

Background: The scientific contribution to the solution of crime cases, or throughout the consequent forensic trials, is a crucial aspect of the justice system. The possibility to extract meaningful information from trace amounts of samples, and to match and validate evidences with robust and unambiguous statistical tests, are the key points of such process. The present report is the authorized disclosure of an investigation, carried out by Attorney General appointment, on a murder case in northern Italy, which yielded the critical supporting evidence for the judicial trial.

Methodology/principal findings: The proportional distribution of 54 chemical elements and the bacterial community DNA fingerprints were used as signature markers to prove the similarity of two soil samples. The first soil was collected on the crime scene, along a corn field, while the second was found in trace amounts on the carpet of a car impounded from the main suspect in a distant location. The matching similarity of the two soils was proven by crossing the results of two independent techniques: a) elemental analysis via inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES) approaches, and b) amplified ribosomal DNA restriction analysis by gel electrophoresis (ARDRA).

Conclusions: Besides introducing the novel application of these methods to forensic disciplines, the highly accurate level of resolution observed, opens new possibilities also in the fields of soil typing and tracking, historical analyses, geochemical surveys and global land mapping.

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Related in: MedlinePlus

Results of the analyses on the soil samples compared with two specimens of soil (CarR, CarL), found respectively on the right and left carpets of the suspect's car floor.a, b) ICP analysis of the content of 54 mineral elements. a): Box and whiskers synthetic representation of the variability of the 12 zones; b) Cluster analysis (single linkage, Euclidean distance) of the data; c) Amplified Ribosomal DNA Restriction Analysis (ARDRA) of the soil bacterial communities. The Neighbour Joining dendrogram resulting from Pearson correlation analysis of the combined three enzymes electrophoretic profiles is shown. The horizontal scale indicates the percent distance. In b) and c) sample replicates are included to show the degree of inter-replicate variability.
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pone-0020222-g002: Results of the analyses on the soil samples compared with two specimens of soil (CarR, CarL), found respectively on the right and left carpets of the suspect's car floor.a, b) ICP analysis of the content of 54 mineral elements. a): Box and whiskers synthetic representation of the variability of the 12 zones; b) Cluster analysis (single linkage, Euclidean distance) of the data; c) Amplified Ribosomal DNA Restriction Analysis (ARDRA) of the soil bacterial communities. The Neighbour Joining dendrogram resulting from Pearson correlation analysis of the combined three enzymes electrophoretic profiles is shown. The horizontal scale indicates the percent distance. In b) and c) sample replicates are included to show the degree of inter-replicate variability.

Mentions: The overall element normalized content in the soils is shown in the box and whiskers graph in Fig. 2a. Samples fell in three non-overlapping groups: the Sardinian soil, the three soils from the crime scene together with the two found on the car carpets, and a third group with the soils from the farther sites. Among the individual element distribution analyses (not shown) some resulted particularly informative, i.e. lithium gave a degree of resolution almost as high as that obtained with the whole set of elements. Results of multivariate exploratory techniques allowed again the grouping of the samples, confirming that the soil found in the car matched with those from the crime scene, and that soils collected within a near range shared a correspondingly high similarity. Fig. 2b shows the Cluster analysis output. The same grouping was obtained with Principal component and classification analysis and Discriminant analysis with canonical analysis (Fig.3).


Chemical elemental distribution and soil DNA fingerprints provide the critical evidence in murder case investigation.

Concheri G, Bertoldi D, Polone E, Otto S, Larcher R, Squartini A - PLoS ONE (2011)

Results of the analyses on the soil samples compared with two specimens of soil (CarR, CarL), found respectively on the right and left carpets of the suspect's car floor.a, b) ICP analysis of the content of 54 mineral elements. a): Box and whiskers synthetic representation of the variability of the 12 zones; b) Cluster analysis (single linkage, Euclidean distance) of the data; c) Amplified Ribosomal DNA Restriction Analysis (ARDRA) of the soil bacterial communities. The Neighbour Joining dendrogram resulting from Pearson correlation analysis of the combined three enzymes electrophoretic profiles is shown. The horizontal scale indicates the percent distance. In b) and c) sample replicates are included to show the degree of inter-replicate variability.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108598&req=5

pone-0020222-g002: Results of the analyses on the soil samples compared with two specimens of soil (CarR, CarL), found respectively on the right and left carpets of the suspect's car floor.a, b) ICP analysis of the content of 54 mineral elements. a): Box and whiskers synthetic representation of the variability of the 12 zones; b) Cluster analysis (single linkage, Euclidean distance) of the data; c) Amplified Ribosomal DNA Restriction Analysis (ARDRA) of the soil bacterial communities. The Neighbour Joining dendrogram resulting from Pearson correlation analysis of the combined three enzymes electrophoretic profiles is shown. The horizontal scale indicates the percent distance. In b) and c) sample replicates are included to show the degree of inter-replicate variability.
Mentions: The overall element normalized content in the soils is shown in the box and whiskers graph in Fig. 2a. Samples fell in three non-overlapping groups: the Sardinian soil, the three soils from the crime scene together with the two found on the car carpets, and a third group with the soils from the farther sites. Among the individual element distribution analyses (not shown) some resulted particularly informative, i.e. lithium gave a degree of resolution almost as high as that obtained with the whole set of elements. Results of multivariate exploratory techniques allowed again the grouping of the samples, confirming that the soil found in the car matched with those from the crime scene, and that soils collected within a near range shared a correspondingly high similarity. Fig. 2b shows the Cluster analysis output. The same grouping was obtained with Principal component and classification analysis and Discriminant analysis with canonical analysis (Fig.3).

Bottom Line: The first soil was collected on the crime scene, along a corn field, while the second was found in trace amounts on the carpet of a car impounded from the main suspect in a distant location.The matching similarity of the two soils was proven by crossing the results of two independent techniques: a) elemental analysis via inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES) approaches, and b) amplified ribosomal DNA restriction analysis by gel electrophoresis (ARDRA).Besides introducing the novel application of these methods to forensic disciplines, the highly accurate level of resolution observed, opens new possibilities also in the fields of soil typing and tracking, historical analyses, geochemical surveys and global land mapping.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biotecnologie Agrarie, Università di Padova, Legnaro (Padova) Italy.

ABSTRACT

Background: The scientific contribution to the solution of crime cases, or throughout the consequent forensic trials, is a crucial aspect of the justice system. The possibility to extract meaningful information from trace amounts of samples, and to match and validate evidences with robust and unambiguous statistical tests, are the key points of such process. The present report is the authorized disclosure of an investigation, carried out by Attorney General appointment, on a murder case in northern Italy, which yielded the critical supporting evidence for the judicial trial.

Methodology/principal findings: The proportional distribution of 54 chemical elements and the bacterial community DNA fingerprints were used as signature markers to prove the similarity of two soil samples. The first soil was collected on the crime scene, along a corn field, while the second was found in trace amounts on the carpet of a car impounded from the main suspect in a distant location. The matching similarity of the two soils was proven by crossing the results of two independent techniques: a) elemental analysis via inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES) approaches, and b) amplified ribosomal DNA restriction analysis by gel electrophoresis (ARDRA).

Conclusions: Besides introducing the novel application of these methods to forensic disciplines, the highly accurate level of resolution observed, opens new possibilities also in the fields of soil typing and tracking, historical analyses, geochemical surveys and global land mapping.

Show MeSH
Related in: MedlinePlus