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Multiple phenotypes in adult mice following inactivation of the Coxsackievirus and Adenovirus Receptor (Car) gene.

Pazirandeh A, Sultana T, Mirza M, Rozell B, Hultenby K, Wallis K, Vennström B, Davis B, Arner A, Heuchel R, Löhr M, Philipson L, Sollerbrant K - PLoS ONE (2011)

Bottom Line: To determine the normal function of the Coxsackievirus and Adenovirus Receptor (CAR), a protein found in tight junctions and other intercellular complexes, we constructed a mouse line in which the CAR gene could be disrupted at any chosen time point in a broad spectrum of cell types and tissues.All knockouts examined displayed a dilated intestinal tract and atrophy of the exocrine pancreas with appearance of tubular complexes characteristic of acinar-to-ductal metaplasia.These results demonstrate that CAR exerts important functions in the physiology of several organs in vivo.

View Article: PubMed Central - PubMed

Affiliation: Ludwig Institutet for Cancer Research, Stockholm Branch, Stockholm, Sweden.

ABSTRACT
To determine the normal function of the Coxsackievirus and Adenovirus Receptor (CAR), a protein found in tight junctions and other intercellular complexes, we constructed a mouse line in which the CAR gene could be disrupted at any chosen time point in a broad spectrum of cell types and tissues. All knockouts examined displayed a dilated intestinal tract and atrophy of the exocrine pancreas with appearance of tubular complexes characteristic of acinar-to-ductal metaplasia. The mice also exhibited a complete atrio-ventricular block and abnormal thymopoiesis. These results demonstrate that CAR exerts important functions in the physiology of several organs in vivo.

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Related in: MedlinePlus

Body weight of cKO and littermate controls after tamoxifen administration.The body weights of F/F;Cre (cKO, n = 20) and F/F littermate control (Ctrl, n = 21) animals were measured weakly following tamoxifen administration given at 4 weeks of age. No difference between the two groups was found. Data are presented as mean ± SEM.
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pone-0020203-g003: Body weight of cKO and littermate controls after tamoxifen administration.The body weights of F/F;Cre (cKO, n = 20) and F/F littermate control (Ctrl, n = 21) animals were measured weakly following tamoxifen administration given at 4 weeks of age. No difference between the two groups was found. Data are presented as mean ± SEM.

Mentions: To evaluate metabolism, fertility, behaviour and locomotion activity, tamoxifen was injected into littermate F/F;Cre (cKO) and F/F control (Ctrl) animals. Animals (cKO, n = 20; controls, n = 21) were weighed weekly for six months, however no difference in weight gain between the two groups was observed (Fig. 3). The cKO animals were fertile and appeared healthy with no increase in mortality compared to controls. The cKO animals showed a tendency for early onset of grey fur, but statistical analysis could not confirm the significance of this finding.


Multiple phenotypes in adult mice following inactivation of the Coxsackievirus and Adenovirus Receptor (Car) gene.

Pazirandeh A, Sultana T, Mirza M, Rozell B, Hultenby K, Wallis K, Vennström B, Davis B, Arner A, Heuchel R, Löhr M, Philipson L, Sollerbrant K - PLoS ONE (2011)

Body weight of cKO and littermate controls after tamoxifen administration.The body weights of F/F;Cre (cKO, n = 20) and F/F littermate control (Ctrl, n = 21) animals were measured weakly following tamoxifen administration given at 4 weeks of age. No difference between the two groups was found. Data are presented as mean ± SEM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108585&req=5

pone-0020203-g003: Body weight of cKO and littermate controls after tamoxifen administration.The body weights of F/F;Cre (cKO, n = 20) and F/F littermate control (Ctrl, n = 21) animals were measured weakly following tamoxifen administration given at 4 weeks of age. No difference between the two groups was found. Data are presented as mean ± SEM.
Mentions: To evaluate metabolism, fertility, behaviour and locomotion activity, tamoxifen was injected into littermate F/F;Cre (cKO) and F/F control (Ctrl) animals. Animals (cKO, n = 20; controls, n = 21) were weighed weekly for six months, however no difference in weight gain between the two groups was observed (Fig. 3). The cKO animals were fertile and appeared healthy with no increase in mortality compared to controls. The cKO animals showed a tendency for early onset of grey fur, but statistical analysis could not confirm the significance of this finding.

Bottom Line: To determine the normal function of the Coxsackievirus and Adenovirus Receptor (CAR), a protein found in tight junctions and other intercellular complexes, we constructed a mouse line in which the CAR gene could be disrupted at any chosen time point in a broad spectrum of cell types and tissues.All knockouts examined displayed a dilated intestinal tract and atrophy of the exocrine pancreas with appearance of tubular complexes characteristic of acinar-to-ductal metaplasia.These results demonstrate that CAR exerts important functions in the physiology of several organs in vivo.

View Article: PubMed Central - PubMed

Affiliation: Ludwig Institutet for Cancer Research, Stockholm Branch, Stockholm, Sweden.

ABSTRACT
To determine the normal function of the Coxsackievirus and Adenovirus Receptor (CAR), a protein found in tight junctions and other intercellular complexes, we constructed a mouse line in which the CAR gene could be disrupted at any chosen time point in a broad spectrum of cell types and tissues. All knockouts examined displayed a dilated intestinal tract and atrophy of the exocrine pancreas with appearance of tubular complexes characteristic of acinar-to-ductal metaplasia. The mice also exhibited a complete atrio-ventricular block and abnormal thymopoiesis. These results demonstrate that CAR exerts important functions in the physiology of several organs in vivo.

Show MeSH
Related in: MedlinePlus