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Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of HBSE on hemolysis induced by B. jararaca venom. B. jararaca venom (30μg/ml) was mixed with HBSE (150-600μg/ml) to give different venom:plant ratio (w/w), as follow: column 1 (1:5); column 2 (1:10); column 3 (1:20) and column 4 (1:50), respectively. The hemolytic assay was performed as described in methods. Data are expressed as means ±S.E.M of two individual experiments (n=3).
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Figure 4: Inhibitory effect of HBSE on hemolysis induced by B. jararaca venom. B. jararaca venom (30μg/ml) was mixed with HBSE (150-600μg/ml) to give different venom:plant ratio (w/w), as follow: column 1 (1:5); column 2 (1:10); column 3 (1:20) and column 4 (1:50), respectively. The hemolytic assay was performed as described in methods. Data are expressed as means ±S.E.M of two individual experiments (n=3).

Mentions: HBSE completely inhibited the hemolysis caused by one MIHD of B.jararaca venom (30μg/ml) at the 1:50 venom:plant ratio (Figure 4, column 4), while a 30% to 80% inhibition was achieved with lower venom:plant ratios (Figure 4, columns 1, 2 and 3). Neither HBSE alone induce hemolysis, nor the hemolytic activity induced by B. jararaca venom mixed with DMSO was affected (data not shown).Figure 4.


Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Inhibitory effect of HBSE on hemolysis induced by B. jararaca venom. B. jararaca venom (30μg/ml) was mixed with HBSE (150-600μg/ml) to give different venom:plant ratio (w/w), as follow: column 1 (1:5); column 2 (1:10); column 3 (1:20) and column 4 (1:50), respectively. The hemolytic assay was performed as described in methods. Data are expressed as means ±S.E.M of two individual experiments (n=3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108466&req=5

Figure 4: Inhibitory effect of HBSE on hemolysis induced by B. jararaca venom. B. jararaca venom (30μg/ml) was mixed with HBSE (150-600μg/ml) to give different venom:plant ratio (w/w), as follow: column 1 (1:5); column 2 (1:10); column 3 (1:20) and column 4 (1:50), respectively. The hemolytic assay was performed as described in methods. Data are expressed as means ±S.E.M of two individual experiments (n=3).
Mentions: HBSE completely inhibited the hemolysis caused by one MIHD of B.jararaca venom (30μg/ml) at the 1:50 venom:plant ratio (Figure 4, column 4), while a 30% to 80% inhibition was achieved with lower venom:plant ratios (Figure 4, columns 1, 2 and 3). Neither HBSE alone induce hemolysis, nor the hemolytic activity induced by B. jararaca venom mixed with DMSO was affected (data not shown).Figure 4.

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus