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Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of HBSE on clotting induced by B. jararaca venom. A. B. jararaca venom (25μg/ml) was mixed with HBSE (250-1250μg/ml) to give different venom:plant ratio (w/w): column 2 (1:10); column 3 (1:20); column 4 (1:50), respectively. B. B. jararaca crude venom (40μg/ml) was mixed with HBSE at venom:plant ratio, column 2 (1:0.05); column 3 (1:0.15); column 4 (1:0.25). Column 1, on both panels represents one MCD of B. jararaca venom mixed with DMSO, instead of HBSE. Data are expressed as means ±S.E.M of two individual experiments (n=4). ##, means that plasma did not clot for the observed 800sec. *, significance level (p <0.05) when compared to column 1.
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Figure 3: Inhibitory effect of HBSE on clotting induced by B. jararaca venom. A. B. jararaca venom (25μg/ml) was mixed with HBSE (250-1250μg/ml) to give different venom:plant ratio (w/w): column 2 (1:10); column 3 (1:20); column 4 (1:50), respectively. B. B. jararaca crude venom (40μg/ml) was mixed with HBSE at venom:plant ratio, column 2 (1:0.05); column 3 (1:0.15); column 4 (1:0.25). Column 1, on both panels represents one MCD of B. jararaca venom mixed with DMSO, instead of HBSE. Data are expressed as means ±S.E.M of two individual experiments (n=4). ##, means that plasma did not clot for the observed 800sec. *, significance level (p <0.05) when compared to column 1.

Mentions: HBSE was able to inhibit B.jararaca-induced clotting upon human plasma (Figure 3A) or upon purified bovine fibrinogen (Figure 3B) in a concentration-dependent manner. The MCD was 25μg/ml or 40μg/ml for plasma or fibrinogen assay, respectively. As observed, at the ratio 1:50 (venom:plant) (Figure 3A, column 4) and 1:0.25 (Figure 3B, column 4), plasma or fibrinogen did not clot. However, at 1:10 or 1:0.05, HBSE did not have any effect, regardless of the clotting method employed (Figure 3, column 2). The HBSE or DMSO alone did not interfere in clotting times (data not shown).Figure 3.


Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Inhibitory effect of HBSE on clotting induced by B. jararaca venom. A. B. jararaca venom (25μg/ml) was mixed with HBSE (250-1250μg/ml) to give different venom:plant ratio (w/w): column 2 (1:10); column 3 (1:20); column 4 (1:50), respectively. B. B. jararaca crude venom (40μg/ml) was mixed with HBSE at venom:plant ratio, column 2 (1:0.05); column 3 (1:0.15); column 4 (1:0.25). Column 1, on both panels represents one MCD of B. jararaca venom mixed with DMSO, instead of HBSE. Data are expressed as means ±S.E.M of two individual experiments (n=4). ##, means that plasma did not clot for the observed 800sec. *, significance level (p <0.05) when compared to column 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3108466&req=5

Figure 3: Inhibitory effect of HBSE on clotting induced by B. jararaca venom. A. B. jararaca venom (25μg/ml) was mixed with HBSE (250-1250μg/ml) to give different venom:plant ratio (w/w): column 2 (1:10); column 3 (1:20); column 4 (1:50), respectively. B. B. jararaca crude venom (40μg/ml) was mixed with HBSE at venom:plant ratio, column 2 (1:0.05); column 3 (1:0.15); column 4 (1:0.25). Column 1, on both panels represents one MCD of B. jararaca venom mixed with DMSO, instead of HBSE. Data are expressed as means ±S.E.M of two individual experiments (n=4). ##, means that plasma did not clot for the observed 800sec. *, significance level (p <0.05) when compared to column 1.
Mentions: HBSE was able to inhibit B.jararaca-induced clotting upon human plasma (Figure 3A) or upon purified bovine fibrinogen (Figure 3B) in a concentration-dependent manner. The MCD was 25μg/ml or 40μg/ml for plasma or fibrinogen assay, respectively. As observed, at the ratio 1:50 (venom:plant) (Figure 3A, column 4) and 1:0.25 (Figure 3B, column 4), plasma or fibrinogen did not clot. However, at 1:10 or 1:0.05, HBSE did not have any effect, regardless of the clotting method employed (Figure 3, column 2). The HBSE or DMSO alone did not interfere in clotting times (data not shown).Figure 3.

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus