Limits...
Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effect of HBSE on proteolysis induced by B. jararaca venom. B. jararaca venom (24μg/ml) was mixed with different concentrations of HBSE (120-480μg/ml) to give venom:plant ratio (w/w) of 1:5 (column 1); 1:10 (column 2); 1:15 (column 3); and 1:20 (column 4), respectively. Data are expressed as means ±S.E.M of three individual experiments (n=3). *, significance level (p <0.05) when compared to column 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3108466&req=5

Figure 2: Inhibitory effect of HBSE on proteolysis induced by B. jararaca venom. B. jararaca venom (24μg/ml) was mixed with different concentrations of HBSE (120-480μg/ml) to give venom:plant ratio (w/w) of 1:5 (column 1); 1:10 (column 2); 1:15 (column 3); and 1:20 (column 4), respectively. Data are expressed as means ±S.E.M of three individual experiments (n=3). *, significance level (p <0.05) when compared to column 1.

Mentions: B. jararaca crude venom was able to hydrolyze azocasein in a concentration-dependent manner with an EC of 12μg/ml (data not shown). Two EC of B. jararaca venom (24μg/ml) were mixed with HBSE to give final venom:plant ratio (w/w) of 1:5, 1:10, 1:15 and 1:20, respectively. As shown in Figure 2, HBSE inhibited proteolytic activity of B. jararaca venom with different potencies; the ratio 1:20 being the most effective, where a complete inhibition was observed. In contrast, the 1:5 ratio inhibited around 60% of proteolysis (Figure 2). HBSE alone (500μg/ml) did not hydrolyze azocasein or DMSO (1%, v/v, final concentration) did not interfere in proteolytic activity of B. jararaca.Figure 2.


Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Inhibitory effect of HBSE on proteolysis induced by B. jararaca venom. B. jararaca venom (24μg/ml) was mixed with different concentrations of HBSE (120-480μg/ml) to give venom:plant ratio (w/w) of 1:5 (column 1); 1:10 (column 2); 1:15 (column 3); and 1:20 (column 4), respectively. Data are expressed as means ±S.E.M of three individual experiments (n=3). *, significance level (p <0.05) when compared to column 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108466&req=5

Figure 2: Inhibitory effect of HBSE on proteolysis induced by B. jararaca venom. B. jararaca venom (24μg/ml) was mixed with different concentrations of HBSE (120-480μg/ml) to give venom:plant ratio (w/w) of 1:5 (column 1); 1:10 (column 2); 1:15 (column 3); and 1:20 (column 4), respectively. Data are expressed as means ±S.E.M of three individual experiments (n=3). *, significance level (p <0.05) when compared to column 1.
Mentions: B. jararaca crude venom was able to hydrolyze azocasein in a concentration-dependent manner with an EC of 12μg/ml (data not shown). Two EC of B. jararaca venom (24μg/ml) were mixed with HBSE to give final venom:plant ratio (w/w) of 1:5, 1:10, 1:15 and 1:20, respectively. As shown in Figure 2, HBSE inhibited proteolytic activity of B. jararaca venom with different potencies; the ratio 1:20 being the most effective, where a complete inhibition was observed. In contrast, the 1:5 ratio inhibited around 60% of proteolysis (Figure 2). HBSE alone (500μg/ml) did not hydrolyze azocasein or DMSO (1%, v/v, final concentration) did not interfere in proteolytic activity of B. jararaca.Figure 2.

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus