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Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus

Chromatography profile of HBSE. Ethanolic extract of H. brasiliense (HBSE) was injected into a Nucleosil MN 120-5 C18 silica column mounted on an HPLC apparatus. The elution was carried out at room temperature using a linear gradient from 10-60% of acetonitrile/water mixture in trifluoracetic acid (0.05%, v/v) at a flow rate of 1ml/min in 30min. Peaks were monitored at 254nm and were denoted as follows: peak 1, chlorogenic acid; peak 2, hyperoside; peak 3, isoquercitrin; peak 4, guaijaverin; peak 5, quercitrin; peak 6, quercetin; peak 7, kaempferol.
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Figure 1: Chromatography profile of HBSE. Ethanolic extract of H. brasiliense (HBSE) was injected into a Nucleosil MN 120-5 C18 silica column mounted on an HPLC apparatus. The elution was carried out at room temperature using a linear gradient from 10-60% of acetonitrile/water mixture in trifluoracetic acid (0.05%, v/v) at a flow rate of 1ml/min in 30min. Peaks were monitored at 254nm and were denoted as follows: peak 1, chlorogenic acid; peak 2, hyperoside; peak 3, isoquercitrin; peak 4, guaijaverin; peak 5, quercitrin; peak 6, quercetin; peak 7, kaempferol.

Mentions: The chemical composition of the ethanolic extract of H. brasiliense was tested by RP-HPLC chromatography. As shown in Figure 1, seven major peaks of phytochemicals were obtained and in relation to their elution times were recorded as follows when compared with a mixture of molecular weight standards (Rocha et al, 1995): chlorogenic acid (1), hyperoside (2), isoquercitrin (3), guaijaverin (4); quercitrin (5), quercetin (6), kaempferol (7).Figure 1.


Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.

Assafim M, de Coriolano EC, Benedito SE, Fernandes CP, Lobo JF, Sanchez EF, Rocha LM, Fuly AL - J Venom Res (2011)

Chromatography profile of HBSE. Ethanolic extract of H. brasiliense (HBSE) was injected into a Nucleosil MN 120-5 C18 silica column mounted on an HPLC apparatus. The elution was carried out at room temperature using a linear gradient from 10-60% of acetonitrile/water mixture in trifluoracetic acid (0.05%, v/v) at a flow rate of 1ml/min in 30min. Peaks were monitored at 254nm and were denoted as follows: peak 1, chlorogenic acid; peak 2, hyperoside; peak 3, isoquercitrin; peak 4, guaijaverin; peak 5, quercitrin; peak 6, quercetin; peak 7, kaempferol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108466&req=5

Figure 1: Chromatography profile of HBSE. Ethanolic extract of H. brasiliense (HBSE) was injected into a Nucleosil MN 120-5 C18 silica column mounted on an HPLC apparatus. The elution was carried out at room temperature using a linear gradient from 10-60% of acetonitrile/water mixture in trifluoracetic acid (0.05%, v/v) at a flow rate of 1ml/min in 30min. Peaks were monitored at 254nm and were denoted as follows: peak 1, chlorogenic acid; peak 2, hyperoside; peak 3, isoquercitrin; peak 4, guaijaverin; peak 5, quercitrin; peak 6, quercetin; peak 7, kaempferol.
Mentions: The chemical composition of the ethanolic extract of H. brasiliense was tested by RP-HPLC chromatography. As shown in Figure 1, seven major peaks of phytochemicals were obtained and in relation to their elution times were recorded as follows when compared with a mixture of molecular weight standards (Rocha et al, 1995): chlorogenic acid (1), hyperoside (2), isoquercitrin (3), guaijaverin (4); quercitrin (5), quercetin (6), kaempferol (7).Figure 1.

Bottom Line: Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose.We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom.This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

View Article: PubMed Central - PubMed

ABSTRACT
Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.

No MeSH data available.


Related in: MedlinePlus