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Screening for tuberculosis and its histological pattern in patients with enlarged lymph node.

Ahmed HG, Nassar AS, Ginawi I - Patholog Res Int (2011)

Bottom Line: Tuberculosis is a major health problem in the Republic of Yemen.All of the 100 specimens were proved as having histopathological pattern of tuberculosis lymphadenitis.The most major histological features were giant cell (88%), caseation (84%), epithelioid cells (80%), granuloma and caseation (68%), lymphocytes (31%), and histiocytes (4%).

View Article: PubMed Central - PubMed

Affiliation: Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.

ABSTRACT
Settings. Tuberculosis is a major health problem in the Republic of Yemen. Tuberculous lymphadenitis is one of the most common forms of extrapulmonary tuberculosis. Therefore, this retrospective descriptive study was conducted in Yemen to investigate the morphological pattern of tuberculous lymphadenitis, as well as to assess the reliability measures of (ZN) Ziehl-Neelsen and fluorescent methods in identification of Mycobacterium tuberculosis. Methodology. One hundred lymph nodes tissue biopsies that were previously diagnosed by conventional histopathology as having tuberculous lymphadenitis were reinvestigated. Five micron in thickness sections were obtained from formalin-fixed paraffin wax processed tissues. The sections were stained using Haematoxylin and eosin (H & E), ZN, and fluorescent methods. Results. All of the 100 specimens were proved as having histopathological pattern of tuberculosis lymphadenitis. The most major histological features were giant cell (88%), caseation (84%), epithelioid cells (80%), granuloma and caseation (68%), lymphocytes (31%), and histiocytes (4%). After staining the specimens with ZN and fluorescent, of the 100 specimens only 3 (3%) and 9 (9%) specimens were found positive, by ZN and fluorescent methods, respectively. Conclusion. Conventional ZN and fluorescent methods have limitations in diagnosis of tuberculous lymphadenitis due to their lower sensitivity. Histopathology remains the most suitable method for the diagnosis of tuberculous lymphadenitis. In cases of suspected tuberculous lymphadenitis, it is advisable to confirm with more sensitive and specific method, such as polymerase chain reaction PCR or immunohistochemistry before reporting the negative results.

No MeSH data available.


Related in: MedlinePlus

Showing description of the study population by lymph node site.
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Related In: Results  -  Collection


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fig1: Showing description of the study population by lymph node site.

Mentions: This is a descriptive study to screen patients with enlarged lymph node for Mycobacterium tuberculosis. The study was conducted in Sana'a, Yemen during the period from 2007 to 2009. One hundred lymph node biopsies were retrieved from 631 lymph node biopsies, which were previously obtained from patients with enlarged lymph nodes. The biopsies' sites were shown in Figure 1. All specimens were formalin-fixed paraffin wax processed tissues. Information regarding each patient was obtained from each patient's file. The specimens were fixed in 10% formalin and then processed by tissue processing machine using the following schedule adopting 24-hour scheduling. Three 5-microns thickness sections were obtained from each patient's block using Rotary Microtome. Of the 3 sections, each one was stained with one staining procedure (haematoxylin and eosin, ZN, or fluorescence).


Screening for tuberculosis and its histological pattern in patients with enlarged lymph node.

Ahmed HG, Nassar AS, Ginawi I - Patholog Res Int (2011)

Showing description of the study population by lymph node site.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3108380&req=5

fig1: Showing description of the study population by lymph node site.
Mentions: This is a descriptive study to screen patients with enlarged lymph node for Mycobacterium tuberculosis. The study was conducted in Sana'a, Yemen during the period from 2007 to 2009. One hundred lymph node biopsies were retrieved from 631 lymph node biopsies, which were previously obtained from patients with enlarged lymph nodes. The biopsies' sites were shown in Figure 1. All specimens were formalin-fixed paraffin wax processed tissues. Information regarding each patient was obtained from each patient's file. The specimens were fixed in 10% formalin and then processed by tissue processing machine using the following schedule adopting 24-hour scheduling. Three 5-microns thickness sections were obtained from each patient's block using Rotary Microtome. Of the 3 sections, each one was stained with one staining procedure (haematoxylin and eosin, ZN, or fluorescence).

Bottom Line: Tuberculosis is a major health problem in the Republic of Yemen.All of the 100 specimens were proved as having histopathological pattern of tuberculosis lymphadenitis.The most major histological features were giant cell (88%), caseation (84%), epithelioid cells (80%), granuloma and caseation (68%), lymphocytes (31%), and histiocytes (4%).

View Article: PubMed Central - PubMed

Affiliation: Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.

ABSTRACT
Settings. Tuberculosis is a major health problem in the Republic of Yemen. Tuberculous lymphadenitis is one of the most common forms of extrapulmonary tuberculosis. Therefore, this retrospective descriptive study was conducted in Yemen to investigate the morphological pattern of tuberculous lymphadenitis, as well as to assess the reliability measures of (ZN) Ziehl-Neelsen and fluorescent methods in identification of Mycobacterium tuberculosis. Methodology. One hundred lymph nodes tissue biopsies that were previously diagnosed by conventional histopathology as having tuberculous lymphadenitis were reinvestigated. Five micron in thickness sections were obtained from formalin-fixed paraffin wax processed tissues. The sections were stained using Haematoxylin and eosin (H & E), ZN, and fluorescent methods. Results. All of the 100 specimens were proved as having histopathological pattern of tuberculosis lymphadenitis. The most major histological features were giant cell (88%), caseation (84%), epithelioid cells (80%), granuloma and caseation (68%), lymphocytes (31%), and histiocytes (4%). After staining the specimens with ZN and fluorescent, of the 100 specimens only 3 (3%) and 9 (9%) specimens were found positive, by ZN and fluorescent methods, respectively. Conclusion. Conventional ZN and fluorescent methods have limitations in diagnosis of tuberculous lymphadenitis due to their lower sensitivity. Histopathology remains the most suitable method for the diagnosis of tuberculous lymphadenitis. In cases of suspected tuberculous lymphadenitis, it is advisable to confirm with more sensitive and specific method, such as polymerase chain reaction PCR or immunohistochemistry before reporting the negative results.

No MeSH data available.


Related in: MedlinePlus