Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death.
Bottom Line: Silibinin caused ROS generation and its effect was inhibited by calpain inhibitor, the general PKC inhibitor GF 109203X, the specific PKCδ inhibitor rottlerin, and catalase.Silibinin induced AIF nuclear translocation and its effect was prevented by calpain inhibitor.Transfection of vector expressing microRNA of AIF prevented the silibinin-induced cell death.
Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.Show MeSH
Related in: MedlinePlus
Mentions: The increase in Bax expression may cause disruption of △ψm to induce cell death. To test the possibility, cells were exposed to silibinin and the △ψm was measured using the fluorescence dye. After silibinin treatment, disruption of △ψm was observed as evidenced by an increase in the proportion of cells with lower fluorescence intensity (Figure 4A). The reduction in △ψm was observed after 3 h of silibinin treatment and remained unchanged even after 12 h (Figure 4B).
Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.