Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death.
Bottom Line: Silibinin caused ROS generation and its effect was inhibited by calpain inhibitor, the general PKC inhibitor GF 109203X, the specific PKCδ inhibitor rottlerin, and catalase.Silibinin induced AIF nuclear translocation and its effect was prevented by calpain inhibitor.Transfection of vector expressing microRNA of AIF prevented the silibinin-induced cell death.
Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.Show MeSH
Related in: MedlinePlus
Mentions: Since numerous death signals converge on mitochondria through the activation of pro-apoptotic members of the Bcl-2 family such as Bax , calpain activation may induce the silibinin-induced cell death through a Bax-dependent pathway. To test this possibility, the effect of silibinin on Bax expression was examined. Silibinin increased Bax expression after 3 h of treatment, which was blocked by the calpain inhibitor (Figure 3).
Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.