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Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death.

Jeong JC, Shin WY, Kim TH, Kwon CH, Kim JH, Kim YK, Kim KH - J. Exp. Clin. Cancer Res. (2011)

Bottom Line: Silibinin caused ROS generation and its effect was inhibited by calpain inhibitor, the general PKC inhibitor GF 109203X, the specific PKCδ inhibitor rottlerin, and catalase.Silibinin induced AIF nuclear translocation and its effect was prevented by calpain inhibitor.Transfection of vector expressing microRNA of AIF prevented the silibinin-induced cell death.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.

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Effect of silibinin on Bax expression. Cells were exposed to 30 μM silibinin for various times and Bax expression was estimated by Western blot analysis. Representative (A) and quantitative (B) results of four independent experiments. (C) Cells were exposed to 30 μM silibinin for 24 h in the presence or absence of 0.5 μM calpain inhibitor (CHO) and Bax expression was estimated by Western blot analysis.
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Figure 3: Effect of silibinin on Bax expression. Cells were exposed to 30 μM silibinin for various times and Bax expression was estimated by Western blot analysis. Representative (A) and quantitative (B) results of four independent experiments. (C) Cells were exposed to 30 μM silibinin for 24 h in the presence or absence of 0.5 μM calpain inhibitor (CHO) and Bax expression was estimated by Western blot analysis.

Mentions: Since numerous death signals converge on mitochondria through the activation of pro-apoptotic members of the Bcl-2 family such as Bax [24], calpain activation may induce the silibinin-induced cell death through a Bax-dependent pathway. To test this possibility, the effect of silibinin on Bax expression was examined. Silibinin increased Bax expression after 3 h of treatment, which was blocked by the calpain inhibitor (Figure 3).


Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death.

Jeong JC, Shin WY, Kim TH, Kwon CH, Kim JH, Kim YK, Kim KH - J. Exp. Clin. Cancer Res. (2011)

Effect of silibinin on Bax expression. Cells were exposed to 30 μM silibinin for various times and Bax expression was estimated by Western blot analysis. Representative (A) and quantitative (B) results of four independent experiments. (C) Cells were exposed to 30 μM silibinin for 24 h in the presence or absence of 0.5 μM calpain inhibitor (CHO) and Bax expression was estimated by Western blot analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3108340&req=5

Figure 3: Effect of silibinin on Bax expression. Cells were exposed to 30 μM silibinin for various times and Bax expression was estimated by Western blot analysis. Representative (A) and quantitative (B) results of four independent experiments. (C) Cells were exposed to 30 μM silibinin for 24 h in the presence or absence of 0.5 μM calpain inhibitor (CHO) and Bax expression was estimated by Western blot analysis.
Mentions: Since numerous death signals converge on mitochondria through the activation of pro-apoptotic members of the Bcl-2 family such as Bax [24], calpain activation may induce the silibinin-induced cell death through a Bax-dependent pathway. To test this possibility, the effect of silibinin on Bax expression was examined. Silibinin increased Bax expression after 3 h of treatment, which was blocked by the calpain inhibitor (Figure 3).

Bottom Line: Silibinin caused ROS generation and its effect was inhibited by calpain inhibitor, the general PKC inhibitor GF 109203X, the specific PKCδ inhibitor rottlerin, and catalase.Silibinin induced AIF nuclear translocation and its effect was prevented by calpain inhibitor.Transfection of vector expressing microRNA of AIF prevented the silibinin-induced cell death.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.

Show MeSH
Related in: MedlinePlus