Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death.
Bottom Line: Silibinin caused ROS generation and its effect was inhibited by calpain inhibitor, the general PKC inhibitor GF 109203X, the specific PKCδ inhibitor rottlerin, and catalase.Silibinin induced AIF nuclear translocation and its effect was prevented by calpain inhibitor.Transfection of vector expressing microRNA of AIF prevented the silibinin-induced cell death.
Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.Show MeSH
Related in: MedlinePlus
Mentions: The silibinin-induced cell death was associated with ROS generation mediated by intracellular Ca2+ . To determine therefore whether ROS production by silibinin is attributed to calpain activation, cells were exposed to silibinin in the presence of calpain inhibitor and ROS generation was measured. As shown in Figure 2A, the silibinin-induced ROS generation was blocked by the calpain inhibitor with potency similar to that of catalase.
Affiliation: Department of Oriental Medicine, Dongguk University, Kyung Ju, 780-714, Korea.